The Myc Gene: Methods and Protocols

ISBN-13: 9781493960170 / Angielski / Miękka / 2016 / 282 str.

Laura Soucek; Nicole M. Sodir

Opis

Myc controls multiple cellular functions, including cell proliferation, growth, differentiation and death, both directly and indirectly, through its modulation of downstream transcriptional programs. In The Myc Gene: Methods and Protocols, experts in the field summarize the standard and novel techniques that allow the studying of Myc mechanism of action in normal and cancer cells, in vitro and in vivo, in one succinct manual. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Spis treści

1. The Myc World Within Reach

Nicole M. Sodir and Laura Soucek

 

2. Methods for the Expression, Purification, Preparation and Biophysical Characterization of Constructs of the c-Myc and Max b-HLH-LZs

Marie-Eve Beaulieu, François-Olivier Mc Duff, Mikaël Bédard, Martin Montagne and Pierre Lavigne

 

3. Analyzing Myc in Cell Transformation and Evolution

Markus Hartl and Klaus Bister

 

4. Identifying Myc Interactors

Romina Ponzielli, William B. Tu, Igor Jurisica,  and Linda Z. Penn

 

5. Detection of c-Myc Protein-Protein Interactions And Phosphorylation Status by Immunoprecipitation

Colin J. Daniel, Xiaoli Zhang and Rosalie C. Sears

 

6. An Efficient Way of Studying Protein–Protein Interactions Involving HIF-α, c-Myc, and Sp1

Kenneth K.-W. To and L. Eric Huang

 

7. Methods for Determining Myc-induced Apoptosis

Dan Lu and Trevor D. Littlewood

 

8. Methods to Study Myc-regulated Cellular Senescence

Vedrana Tabor, Matteo Bocci and Lars-Gunnar Larsson

 

9. Chromatin Immunoprecipitation Assays for Myc and N-Myc

Bonnie L. Barrilleaux, Rebecca Cotterman, and Paul S. Knoepfler

 

10. Methods to Quantify microRNAs in the Myc Gene Network for Posttranscriptional Gene Repression

Rui Song, Nicole Sponer, and Lin He

 

11. Genome-wide Analysis of c-MYC-regulated mRNAs and miRNAs, and c-MYC DNA-binding by Next Generation Sequencing

Rene Jackstadt, Antje Menssen and Heiko Hermeking

 

12. A High Throughput siRNA Screening Platform to Identify MYC Synthetic Lethal Genes as Candidate Therapeutic Targets

Carla Grandori

 

13. Investigating Myc-dependent Translational Regulation in Normal and Cancer Cells

John T. Cunningham, Michael Pourdehnad, Craig R. Stumpf, and Davide Ruggero

 

14. Studying Myc’s Role in Metabolism Regulation

Anne Le and Chi V. Dang

 

15. Generation of a Tetracycline Regulated Mouse Model of MYC-induced T-cell Acute Lymphoblastic Leukemia

Kavya Rakhra and Dean W. Felsher

 

16. Methods to Assess Myc Function in Intestinal Homeostasis, Regeneration and Tumourigenesis

David J Huels, Patrizia Cammareri, Rachel A Ridgway, Jan P Medema and Owen J Sansom

 

17. Detection of c-myc Amplification in Formalin-fixed Paraffin-Embedded Tumor Tissue by Chromogenic in situ Hybridization (CIHS)

Natasa Todorovic-Rakovic

 

18. Cell-Based Methods for the Identification of MYC-Inhibitory Small Molecules

Catherine A. Burkhart, Michelle Haber, Murray D. Norris, Andrei V. Gudkov, and Mikhail A. Nikiforov