The Myc Gene: Methods and Protocols

ISBN-13: 9781071614785 / Angielski / Miękka / 2021

Soucek, Laura

Opis

This second edition provides new and updated chapters detailing recent advances in MYC research and current techniques. Chapters guide readers through protocols on how to express and purify MYC protein, X-ray crystallography, NMR, techniques to study how MYC is modified, apoptosis, senescence, proliferation, metabolic changes, translation, tumorigenesis,reprogramming, and clinical application of MYC studies.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge,  The Myc Gene: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.  

Spis treści

1. An “-omycs” Toolbox to Work with MYC

Jonathan R. Whitfield and Laura Soucek

  2. Methods of Expression, Purification and Preparation of the c-Myc b-HLH-LZ for its Biophysical Characterization Patrick Delattre, Martin Montagne, and Pierre Lavigne  

3. Biophysical and Structural Methods to Study the bHLHzip Region of Human c-MYC

Giovanna Zinzalla

 

4. Identifying and Validating MYC:protein Interactors in Pursuit of Novel anti-MYC Therapies

Diana Resetca, Alannah S. MacDonald, Tristan M. G. Kenney, Yong Wei, Cheryl H. Arrowsmith, Brian Raught, and Linda Z. Penn

 

5. Detection of Post-Translational Modifications on MYC

Colin J. Daniel, Xiao-Xin Sun, Yingxiao Chen, Xiaoli Zhang, Mu-Shui Dai, and Rosalie C. Sears

 

6. MYC Analysis in Cancer and Evolution

Markus Hartl and Klaus Bister

 

7. Genome-wide analysis of c-MYC-regulated mRNAs and miRNAs, and c-MYC DNA-binding by Next Generation Sequencing

Rene Jackstadt, Markus Kaller, Antje Menssen,and Heiko Hermeking

 

8. Analysis of Myc Chromatin Binding by Calibrated ChIP-seq Approach

Donald P. Cameron, Vladislav Kuzin, and Laura Baranello

 

9. A High-Throughput Chromatin Immunoprecipitation Sequencing Approach to Study the Role of MYC on the Epigenetic Landscape

Luca Fagnocchi and Alessio Zippo

 

10. Methods for Determining Myc-induced Apoptosis

Dan Lu, Catherine Wilson, and Trevor D. Littlewood

 

11. Measuring MYC-mediated Metabolism in Tumorigenesis

Hsin-Yao Tang, Aaron R. Goldman, Xue Zhang, David W. Speicher, and Chi V. Dang

 

12. Methods to study Myc-regulated Cellular Senescence – An Update

Fan Zhang, Wesam Bazzar, Mohammad Alzrigat, and Lars-Gunnar Larsson

 

13. Examining Myc-Dependent Translation Changes in Cellular Homeostasis and Cancer

Joanna R. Kovalski, Yichen Xu, and Davide Ruggero

 

14. A Versatile in vivo System to Study Myc in Cell Reprogramming

Elena Senis, Lluc Mosteiro, Dirk Grimm, and Maria Abad

15. Using Flow Cytometry to Study Myc’s Role in Shaping the Tumor Immune Microenvironment

Sílvia Casacuberta-Serra

 

16. Generation of a Tetracycline Regulated Mouse Model of MYC-Induced

T-Cell Acute Lymphoblastic Leukemia

Wadie D. Mahauad-Fernandez, Kavya Rakhra, and Dean W. Felsher

 

17. Chromogenic in Situ Hybridization (CISH) as a Method for Detection of C-Myc Amplification in Formalin-Fixed Paraffin-Embedded Tumor Tissue: An Update

Nataša Todorović-Raković

 

18. MYC Copy Number Detection in Clinical Samples using a Digital DNA-Hybridization and Detection Method

Zighereda Ogbah, Francesco Mattia Mancuso, and Ana Vivancos

 

19. Cell-Based Methods for the Identification of Myc-Inhibitory Small Molecules

Catherine A. Burkhart, Michelle Haber, Murray D. Norris, Andrei V. Gudkov, and Mikhail A. Nikiforov