2. Methods of Expression, Purification and Preparation of the c-Myc b-HLH-LZ for its Biophysical Characterization Patrick Delattre, Martin Montagne, and Pierre Lavigne
3. Biophysical and Structural Methods to Study the bHLHzip Region of Human c-MYC
Giovanna Zinzalla
4. Identifying and Validating MYC:protein Interactors in Pursuit of Novel anti-MYC Therapies
Diana Resetca, Alannah S. MacDonald, Tristan M. G. Kenney, Yong Wei, Cheryl H. Arrowsmith, Brian Raught, and Linda Z. Penn
5. Detection of Post-Translational Modifications on MYC
Colin J. Daniel, Xiao-Xin Sun, Yingxiao Chen, Xiaoli Zhang, Mu-Shui Dai, and Rosalie C. Sears
6. MYC Analysis in Cancer and Evolution
Markus Hartl and Klaus Bister
7. Genome-wide analysis of c-MYC-regulated mRNAs and miRNAs, and c-MYC DNA-binding by Next Generation Sequencing
Rene Jackstadt, Markus Kaller, Antje Menssen,and Heiko Hermeking
8. Analysis of Myc Chromatin Binding by Calibrated ChIP-seq Approach
Donald P. Cameron, Vladislav Kuzin, and Laura Baranello
9. A High-Throughput Chromatin Immunoprecipitation Sequencing Approach to Study the Role of MYC on the Epigenetic Landscape
Luca Fagnocchiand Alessio Zippo
10. Methods for Determining Myc-induced Apoptosis
Dan Lu, Catherine Wilson, and Trevor D. Littlewood
11. Measuring MYC-mediated Metabolism in Tumorigenesis
Hsin-Yao Tang, Aaron R. Goldman, Xue Zhang, David W. Speicher, and Chi V. Dang
12. Methods to study Myc-regulated Cellular Senescence – An Update
Fan Zhang, Wesam Bazzar, Mohammad Alzrigat, and Lars-Gunnar Larsson
13. Examining Myc-Dependent Translation Changes in Cellular Homeostasis and Cancer
Joanna R. Kovalski, Yichen Xu, and Davide Ruggero
14. A Versatile in vivo System to Study Myc in Cell Reprogramming
Elena Senis, Lluc Mosteiro, Dirk Grimm, and Maria Abad
15. Using Flow Cytometry to Study Myc’s Role in Shaping the Tumor Immune Microenvironment
Sílvia Casacuberta-Serra
16. Generation of a Tetracycline Regulated Mouse Model of MYC-Induced
T-Cell Acute Lymphoblastic Leukemia
Wadie D. Mahauad-Fernandez, Kavya Rakhra, and Dean W. Felsher
17. Chromogenic inSitu Hybridization (CISH) as a Method for Detection of C-Myc Amplification in Formalin-Fixed Paraffin-Embedded Tumor Tissue: An Update
Nataša Todorović-Raković
18. MYC Copy Number Detection in Clinical Samples using a Digital DNA-Hybridization and Detection Method
Zighereda Ogbah, Francesco Mattia Mancuso, and Ana Vivancos
19. Cell-Based Methods for the Identification of Myc-Inhibitory Small Molecules
Catherine A. Burkhart, Michelle Haber, Murray D. Norris, Andrei V. Gudkov, and Mikhail A. Nikiforov
This second edition provides new and updated chapters detailing recent advances in MYC research and current techniques. Chapters guide readers through protocols on how to express and purify MYC protein, X-ray crystallography, NMR, techniques to study how MYC is modified, apoptosis, senescence, proliferation, metabolic changes, translation, tumorigenesis,reprogramming, and clinical application of MYC studies.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, The Myc Gene: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.