The Making and Un-Making of Neuronal Circuits in Drosophila

ISBN-13: 9781493962396 / Angielski / Miękka / 2016 / 278 str.

Bassem A. Hassan

Opis

Organized in sections on Physiology and Behavior, this Neuromethods series volume explores ideas and concepts behind the methods, tools, and tricks currently being utilized to decode the secrets of the brain of the small fruit fly, drosophila melanogaster .

Spis treści

Part I: Anatomy

 

1. Deciphering the Adult Brain: From Neuroanatomy to Behavior

            Matthias Landgraf and Jan Felix Evers

 

Part II: Physiology

 

6. Studying Synaptic Transmission at the Drosophila Neuromuscular Junction Using Advanced FM 1-43 Technology

            Ana Clara Fernandes, Valerie Uytterhoeven, and Patrik Verstreken

 

7. Optical Recording of Visually Evoked Activity in the Drosophila Central Nervous System

            Dierk F. Reiff

 

Part III: Behavior

 

8. Behavioral Analysis of Navigation Behaviors in the Drosophila Larva

            Matthieu Louis, Moraea Phillips, Mariana Lopez-Matas, and Simon Sprecher

 

9. Drosophila as a Genetic Model to Investigate Motion Vision

            Daryl M. Gohl, Marion A. Silies, and Thomas R. Clandinin

 

Part IV: Futures

 

10. Using Primary Neuron Cultures of Drosophila to Analyze Neuronal Circuit Formation and Function

            Andreas Prokop, Barbara Küppers-Munther, and Natalia Sánchez-Soriano

 

11. Molecular Profiling of Neural Stem Cells in Drosophila melanogaster

            Elizabeth E. Caygill, Katrina S. Gold, and Andrea H. Brand

 

12. Out with the Brain: Drosophila Whole-Brain Explant Culture

            Marta Koch and Bassem A. Hassan

            Liesbeth Zwarts, Jason Clements, and Patrick Callaerts

 

2. Genetically Encoded Markers for Drosophila Neuroanatomy

            Xiao-jiang Quan, Ariane Ramaekers, and Bassem A. Hassan

 

3. Subcellular Resolution Imaging in Neural Circuits

            W. Ryan Williamson, Chih-Chiang Chan, and P. Robin Hiesinger

 

4. In Vivo Single Cell Labeling Techniques

            Chih-Fei Kao and Tzumin Lee

 

5. Neuronal Morphology in the Drosophila Embryo: Visualization, Digital Reconstruction, and Quantification

            Matthias Landgraf and Jan Felix Evers

 

Part II: Physiology

 

6. Studying Synaptic Transmission at the Drosophila Neuromuscular Junction Using Advanced FM 1-43 Technology

            Ana Clara Fernandes, Valerie Uytterhoeven, and Patrik Verstreken

 

7. Optical Recording of Visually Evoked Activity in the Drosophila Central Nervous System

            Dierk F. Reiff

 

Part III: Behavior

 

8. Behavioral Analysis of Navigation Behaviors in the Drosophila Larva

            Matthieu Louis, Moraea Phillips, Mariana Lopez-Matas, and Simon Sprecher

 

9. Drosophila as a Genetic Model to Investigate Motion Vision

            Daryl M. Gohl, Marion A. Silies, and Thomas R. Clandinin

 

Part IV: Futures

 

10. Using Primary Neuron Cultures of Drosophila to Analyze Neuronal Circuit Formation and Function

            Andreas Prokop, Barbara Küppers-Munther, and Natalia Sánchez-Soriano

 

11. Molecular Profiling of Neural Stem Cells in Drosophila melanogaster

            Elizabeth E. Caygill, Katrina S. Gold, and Andrea H. Brand

 

12. Out with the Brain: Drosophila Whole-Brain Explant Culture

            Marta Koch and Bassem A. Hassan

Blurb

The small fruit fly, Drosophila melanogaster, has for over a century now had a large impact on biological and biomedical research; however, our knowledge of the fly brain has lagged significantly behind our understanding of other aspects of its development, physiology, and function.  In The Making and Un-Making of Neuronal Circuits in Drosophila, innovative expert neuroscientists in the field present the ideas and concepts behind the methods, tools, and tricks that are currently being utilized to decode the secrets of this valuable insect’s brain.  Focused on the concept of a neuronal circuit, defined as a series of synaptically connected neurons subservient to a particular behavioral modality, this volume contains chapters dealing with anatomical analysis with a focus on cellular and sub-cellular morphologies.  These detailed approaches fall under the headings of “Physiology” and “Behavior”, conveniently divided the book into two sections.  Written in the easy-to-follow Neuromethods series format, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results.

 

Inventive and accessible, The Making and Un-Making of Neuronal Circuits in Drosophila provides the information and tools necessary to carry out current experiments and, more importantly, further advance the progress of the Drosophila neurobiology field and neurobiology in general.