The Maize Handbook

ISBN-13: 9780387947358 / Angielski / Miękka / 1996 / 761 str.

Opis

The Maize Handbook represents the collective efforts of the maize research community to enumerate the key steps of standard procedures and to disseminate these protocols for the common good. Although the material in this volume is drawn from experience with maize, many of the procedures, protocols, and descriptions are applicable to other higher plants, particularly to other grasses. The power and resolution of experiments with maize depend on the wide range of specialized genetic techniques and marked stocks; these materials are available today as the culmination of nearly 100 years of genetic research. A major goal of this volume is to introduce this genetical legacy and to highlight current stock construction programs that will soon benefit our work, e. g. high-density RFLP maps, deletion stocks, etc. Both stock construction and maintenance are relatively straightforward in maize as a result of the ease of crossing and the longevity of stored seeds. Crossing is facilitated by the separate staminate (tassel) and pistillate (ear) flowers, a feature almost unique to maize. On the other hand, many of the genetic methodologies utilized with maize, including the precision of record keeping, can be adapted to other plants. Facile communication and a spirit of co-operation have characterized the maize genetics community since its earliest days. Starting in the 1930s, institutions such as annual Maize Genetics Cooperation Newsletter, the Maize Genetics Stock Center, and the annual maize genetics meeting provide continuity to the field.

Recenzje

"The book is certainly one that every university and research station library should buy, and every plant geneticist should have access to." Plant Genetics Newsletter
"This handbook sets the standard and is an essential work for anyone doing maize research of any type." Choice

Spis treści

1. Fertilization Embryogeny in Maize.- 2. The Maize Shoot.- 3. The Maize Leaf.- 4. The Maize Root.- 5. Morphology and Development of the Tassel and Ear.- 6. Gametogenesis in Maize.- 7. The Patterns of Plant Structures in Maize.- 8. Genetics and the Morphological Evolution of Maize.- 9. Overview of Key Steps in Aleurone Development.- 10. Light Microscopy I: Dissection Microtechnique.- 11. Light Microscopy II: Observation, Photomicrography, and Image Analysis.- 12. Scanning Electron Microscopy.- 13. Transmission Electron Microscopy: Chemical Fixation, Freezing Methods, and Immunolocalization.- 14. Techniques for Histology of Maize Megaspores and Embryo Sacs.- 15. Indirect Immunofluorescence: Localization of the Cytoskeleton.- 16. Immunocytochemistry for Light and Electron Microscopy.- 17. Immunolocalization of Nuclear Proteins.- 18. In situ Hybridization.- 19. Photography of Maize.- 20. Genetic Experiments Mapping.- 21. Growing Maize for Genetic Studies.- 22. A Nine-Step Way to Characterize a Morphological Mutant.- 23. Mutagenesis.- 24. Gene Tagging with Ac/Ds Elements in Maize.- 25. Using Cytogenetics to Enhance Transposon Tagging with Ac Throughout the Maize Genome.- 26. Transposon Tagging with Spm.- 27. Transposon Tagging with Mutator.- 28. Mapping Genes with Recombinant Inbreds.- 29. The Placement of Genes Using waxy-Marked Reciprocal Translocations.- 30. Chimeras for Genetic Analysis.- 31. The Use of Clonal Sectors for Lineage and Mutant Analysis.- 32. Use of Segmental Aneuploids for Mutant Analysis.- 33. Biased Transmission of Genes and Chromosomes.- 34. Anthocyanin Genetics.- 35. Cloned Anthocyanin Genes and Their Regulation.- 36. Maize and Puccinia sorghi-Marked: A System for the Study of the Genetic and Molecular Basis of Host-Pathogen Interactions.- 37. Disease Lesion Mutants.- 38. Classification of Pollen Abortion in the Field.- 39. Genetic Fine Structure as Revealed in Pollen Assays.- 40. Genetic Fine Structure from Testcross Progeny Analysis.- 41. Trisomic Manipulation.- 42. B-A Translocation Manipulation.- 43. Locating Recessive Genes to Chromosome Arm with B-A Translocations.- 44. Dosage Analysis Using B-A Translocations.- 45. Marker Systems for B-A Translocations.- 46. Construction of Compound B-A Translocations.- 47. A-B-A Compound Chromosomes.- 48. Comprehensive List of B-A Translocations in Maize.- 49. Chromosomal Translocations Involving the Nucleolus Organizer Region or Satellite of Chromosome 6.- 50. Inversions and List of Inversions Available.- 51. Use of Maize Monosomics for Gene Localization and Dosage Studies.- 52. Marker Systems for r-x1.- 53. Translocations as Genetic Markers.- 54. A-A Translocations: Breakpoints and Stocks.- 55. Segmental Aneuploid Analysis.- 56. Directed Synthesis of Segmental Transpositions.- 57. Practical Aspects of Haploid Production.- 58. Indeterminate Gametophyte (ig): Biology and Use.- 59. Production of a Ploidy Series.- 60. Absorption Cytophotometry of Nuclear DNA.- 61. Flow Cytometry for Endosperm Nuclear DNA.- 62. Allelism Testing of Lethal Mutations.- 63. Analysis of Cytoplasmically Inherited Mutants.- 64. Male Sterility and Restorer Genes in Maize.- 65. Inbred Lines of Maize and Their Molecular Markers.- 66. Traditional Analysis of Maize Pachytene Chromosomes.- 67. Techniques for Preparing Whole-Mount Spreads of Maize Pachytene Chromosome Complements for Electron-Microscopic Visualization of Synaptonemal Complex Structures.- 68. A Smear Technique for the Study of Meiosis in Pollen Mother Cells of Maize.- 69. Protocol for Preparing Maize Macrospore Mother Cells for the Study of Female Meiosis and Embryo-sac Development.- 70. Preparing a Suspension of Microsporocytes for Spreading and Electron Microscopy.- 71. Three-dimensional Fluorescence Microscopy of Maize Chromosomes.- 72. Chromosomal Behavior During Microsporogenesis.- 73. A Staining Procedure for Pollen Grain Chromosomes of Maize.- 74. A Technique for the Preparation of Somatic Chromosomes of Maize.- 75. A Technique for Somatic Chromosome Preparation and C-banding of Maize.- 76. Duplications.- 77. Deficiency Analysis.- 78. The Gametophyte Factors of Maize.- 79. Ring Chromosomes.- 80. In situ Hybridization of DNA and RNA Probes to Maize Chromosomes.- 81. Analysis of Traits with Complex Inheritance in Maize Using Molecular Markers.- 82. Heterofertilization.- 83. Isolation of Small Nuclear RNAs.- 84. Plant DNA Miniprep and Microprep: Versions 2.1–2.3.- 85. Urea-based Plant DNA Miniprep.- 86. High-Molecular-Weight Plant DNA Preparation for CHEF Gel Analysis.- 87. Preparation of High-Molecular-Weight Maize DNA and Analysis by Pulsed-Field Gel Electrophoresis.- 88. Isolation of Genomic DNA from Calli.- 89. Isolation of DNA from Immature Cobs.- 90. Preparation of Nucleic Acids from Maize Microspores and Pollen.- 91. Preparation of DNA and RNA from Leaves: Expanded Blades and Separated Bundle Sheath and Mesophyll Cells.- 92. Isolation of RNA from Wx and wx Endosperms.- 93. RNA Isolation from Electroporated Protoplasts.- 94. Procedures for Isolating Mitochondria and Mitochondrial DNA and RNA.- 95. Isolation of Maize Chloroplasts and Chloroplast DNA.- 96. In vitro Capping of Maize Mitochondrial RNA and Transcription Initiation Site Characterization by RNase Protection.- 97. Editors’ Note.- 98. Southern Blots of Maize Genomic DNA.- 99. Southern Blot Hybridization.- 100. Northern Blotting.- 101. RNase Protection Assay.- 102. Genomic Sequencing in Maize.- 103. The Polymerase Chain Reaction: Applications to Maize Transposable Elements.- 104. In vitro Synthesis of Capped and Polyadenylated mRNA for Translation Studies in vitro and in vivo.- 105. Construction of a Genomic Library in Lambda Phage.- 106. Agroinfection.- 107. Polyethylene Glycol-mediated DNA Uptake into Maize Protoplasts.- 108. DNA Delivery into Maize Cell Cultures Using Silicon Carbide Fibers.- 109. Transient Gene Expression Assay by Electroporation of Maize Protoplasts.- 110. Assay of Bacterial Chloramphenicol Acetyl Transferase in Transformed Maize Tissues.- 111. Assay of Firefly Luciferase in Transformed Maize Tissues.- 112. Storage of Frozen Maize Tissue.- 113. Maize Methods—Starch Biosynthetic Genes.- 114. Identifying and Characterizing the TATA Box Promoter Sequence Element in a Maize Nuclear Gene.- 115. Promoters.- 116. Introns.- 117. Characterization of Zein Genes and Their Regulation in Maize Endosperms.- 118. Overview of Cloning Genes Using Transposon Tagging.- 119. How RFLP Loci Can Be Used to Assist Transposon-Tagging Efforts.- 120. Regeneration of Plants from Somatic Cell Cultures: Applications for in vitro Genetic Manipulation.- 121. Initiation, Maintenance, and Plant Regeneration of Type II Callus and Suspension Cells.- 122. Production of Transgenic Maize Plants via Microprojectile-Mediated Gene Transfer.- 123. In vitro Selection.- 124. Selection of Stable Transformants from Black Mexican Sweet Maize Suspension Cultures.- 125. Maize Protoplast Culture.- 126. Anther and Microspore Culture.- 127. In vitro Culture of Maize Kernels.- 128. In vitro Ear Culture System.- 129. Maize Inflorescence Culture.- 130. Shoot Meristem Culture.- 131. Establishment and Culture of Maize Endosperm.- 132. In vitro Pollen Germination.- 133. Axillary Bud in vitro Culture: Asexual Propagation of Maize.