The Immune Synapse: Methods and Protocols

ISBN-13: 9781071631348 / Angielski

Cosima T. Baldari; Michael L. Dustin

Opis

This new collection features the most up-to-date essential protocols that are currently being used to study the immune synapse. Beginning with methods for making biophysical measurements, the volume continues by covering the cell biology of synapses, methods for advanced substrate engineering, mechanobiology topics, new technologies to describe and manipulate synaptic components, as well as methods related to sites of action and immunotherapy. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and fully updated, The Immune Synapse: Methods and Protocols, Second Edition serves as an ideal practical guide for researchers working in this dynamic field. 

Chapters 5, 11, 18, 27, 30, and 32 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Spis treści

1. Measuring the Co-Localization and Dynamics of Mobile Proteins in Live Cells Undergoing Signaling Responses

            Sarah A. Shelby, Thomas R. Shaw, and Sarah L. Veatch

 

2. Fluorescence-Based Measurements of Two-Dimensional Affinity in Membrane Interfaces

            Tommy Dam, Manto Chouliara, and Peter Jönsson

 

3. Surfaces for Study of Receptor Dynamics on T Cells

            James McColl and David Klenerman

 

4. Molecular Dynamics Simulations of Immune Receptors and Ligands

            Prithvi R. Pandey, Bartosz Rózycki, and Thomas R. Weikl

 

5. Investigating Diffusion Dynamics and Interactions with Scanning Fluorescence Correlation Spectroscopy (sFCS)

            Alexander M. Mørch and Falk Schneider

 

6. Combined FRET-FLIM and NAD(P)H FLIM to Analyze B Cell Receptor Signaling Induced Metabolic Activity of Germinal Center B Cells In Vivo

            Carolin Ulbricht, Ruth Leben, Yu Cao, Raluca A. Niesner, and Anja E. Hauser

 

7. Measurement of Molecular Height Using Cell Surface Optical Profilometry (CSOP)

            Sungmin Son and Daniel A. Fletcher

 

8. Observing Membrane and Cell Adhesion via Reflection Interference Contrast Microscopy (RICM)

            Ahmed Abdelrahman, Ana-Sunčana Smith, and Kheya Sengupta

 

9. En-Face-Imaging of T Cell-Dendritic Cell Immunological Synapses

            Alexander Leithner, Jack Merrin, and Michael Sixt

 

10. High and Super Resolution Imaging of Cell-Cell Interfaces

            Julia Sajman and Eilon Sherman

 

11. Separation of Single Core and Multicore Lytic Granules by Subcellular Fractionation and Immuno-Isolation

            Claudia Schirra, Nadia Alawar, Ute Becherer, and Hsin-Fang Chang

 

12. Microvillar Cartography: A Super-Resolution Single-Molecule Imaging Method to Map the Positions of Membrane Proteins with Respect to Cellular Surface Topography

            Shirsendu Ghosh, Andres Alcover, and Gilad Haran

 

13. T Cell Immunological Synaptosomes: Definition and Isolation

            Hye-Ran Kim, Jeong-Su Park, Na-Young Kim, and Chang-Duk Jun

 

14. Dynamics of Immune Cell Microvilli

            En Cai

 

15. Visualization of Myddosome Assembly in Live Cells

            Fakun Cao and Marcus J. Taylor

 

16. Detection of Telomere Transfer at Immunological Synapse

            Alessio Lanna and Clara D’Ambra

 

17. Bottom-Up Assembly of Bio-Inspired, Fully Synthetic Extracellular Vesicles

            Meline Macher, Ilia Platzman, and Joachim P. Spatz

 

18. A DNA Origami-Based Biointerface to Interrogate the Spatial Requirements for Sensitized T-Cell Antigen Recognition

            Joschka Hellmeier, René Platzer, Johannes B. Huppa, and Eva Sevcsik

 

19. Leveraging DNA Origami to Study Phagocytosis

            Wyatt D. Miller, Nadja Kern, Shawn M. Douglas, and Meghan A. Morrissey

 

20. Fabrication of Nanoscale Arrays to Study the Effect of Ligand Arrangement on Inhibitory Signaling in NK Cells

            Guillaume Le Saux, Esti Toledo-Ashkenazi, and Mark Schvartzman

 

21. Measurement of Forces for Trans-Endocytosis at Dorsal and Ventral Sides of the Cell

            Seungman Park and Yun Chen

 

22. Functionalized Lipid Droplets and Microfluidics Approach to Study Immune Cell Polarity In Vitro

            Judith Pineau, Léa Pinon, Jacques Fattaccioli, and Paolo Pierobon

 

23. Quantifying Immune Cell Force Generation Using Traction Force Microscopy

            Marcel Issler, Huw Colin-York, and Marco Fritzsche

 

24. Characterizing Biophysical Parameters of Single TCR-pMHC Interactions Using Optical Tweezers

            Hannah M. Stephens, Evan Kirkpatrick, Robert J. Mallis, Ellis L. Reinherz, and Matthew J. Lang

 

25. Isolation of the B Cell Immune Synapse for Proteomic Analysis

            Diogo M. Cunha, Sara Hernández-Pérez, and Pieta K. Mattila

 

26. Analyzing Single Cell Secretions by ‘Shadow Imaging’

            Ashley R. Ambrose, Khodor S. Hazime, and Daniel M. Davis

 

27. Exploiting the RUSH System to Study Lytic Granule Biogenesis in Cytotoxic T Lymphocytes

            Nagaja Capitani, Chiara Cassioli, Keerthana Ravichandran, and Cosima T. Baldari

 

28. Interactions of Tissue Resident T Cells

            Rut Mora-Buch, Hasan Akbaba, and Shannon K. Bromley

 

29. Live Imaging of CAR T Cell Ca²⁺ Signals in Tumor Slices Using Confocal Microscopy

            David Espie, Sarah Barrin, Irena Rajnpreht, Lene Vimeux, and Emmanuel Donnadieu

 

30. Measuring CTL Lytic Granule Secretion and Target Cell Membrane Repair by Fluorescent Lipophilic Dye Uptake at the Lytic Synapse

            Sabina Müller, Liza Filali, Marie-Pierre Puissegur, and Salvatore Valitutti

 

31. In Vitro Generation of Human Tolerogenic Monocyte-Derived Dendritic Cells

            Catharien M.U. Hilkens, Julie Diboll, Fiona Cooke, and Amy E. Anderson

 

32. Methods of Machine Learning-Based Chimeric Antigen Receptor Immunological Synapse Quality Quantification

            Julian Gan, Jong Hyun Cho, Ryan Lee, Alireza Naghizadeh, Ling Yue Poon, Ethan Wang, Zachary Hui, and Dongfang Liu

 

33. Imaging CAR-T Synapse as a Quality Control for CAR Engineering

            Qian Xiao and Xiaolei Su