The Handbook of Polyhydroxyalkanoates: Microbial Biosynthesis and Feedstocks

ISBN-13: 9780367275594 / Angielski / Twarda / 2020 / 452 str.

Martin Koller

Opis

This volume focuses on feedstock aspects, enzymology, metabolism and genetic engineering of PHA biosynthesis and covers mechanisms of PHA biosynthesis, enhancement of PHA biosynthesis in bio-technological and microstructure terms and performance enhancement of PHAs over established petrol-based plastics on industrial scale.

Spis treści

Chapter 1: Monomer-Supplying Enzymes for Polyhydroxyalkanoate Biosynthesis1.1Introduction1.2PHA Biosynthesis Pathways and Related Enzymes1.3Monomer-Supplying Enzymes1.4Monomer-Supplying Pathways and Enzymes Involved1.5Conclusions and OutlookReferences Chapter 2: PHA Granule-Associated Proteins and their Diverse Functions2.1Introduction2.2Granule Assembly Models2.3GAPs with Enzymatic Activity: PHA Synthases and Depolymerases2.4Non-Enzymatic GAPs: Transcriptional Regulators and Phasins2.5Functional Diversity of Phasins2.6What Makes a Phasin a Phasin?2.7Biotechnological Applications of GAPs2.8Conclusions and OutlookReferences Chapter 3: Genomics of PHA Synthesizing Bacteria3.1Introduction3.2Short-Chain-Length PHA (scl-PHA) Producing Bacteria3.3Medium-Chain-Length PHA (mcl-PHA) Producing Bacteria3.4Scl-co-mcl-Copolymer Producers3.5Genomics of mcl-PHA Producing Bacteria3.6The Genomics of mcl-PHA Metabolism3.7Mcl-PHA Synthesis from Vegetable Oils and Fats3.8Genome Analysis of Halomonas Species3.9Genome Analysis of Paracoccus Species3.10The PHA Production Machinery in Pseudomonas putida, Cupriavidus necator, Halomonas spp. and Paracoccus spp.3.11Domain Organization and Structural Comparison of PhaC from Cupriavidus necator, Halomonas lutea and Paracoccus denitrificansReferences Chapter 4: Molecular Basis of Medium-Chain Length-PHA Metabolism of Pseudomonas putida4.1Pseudomonas putida, a Model Bacterium for the Production of Medium-Chain-Length PHA4.2The PHA Cycle and its Key Proteins4.3Metabolic Pathways Involved in mcl-PHA Production in P. putida4.4PHA Metabolism Regulation4.5Conclusions and OutlookReferences Chapter 5: Production of Polyhydroxyalkanoates by Paraburkholderia and Burkholderia species: A Journey from the Genes through Metabolic Routes to their Biotechnological Applications5.1Introduction5.2PHA Synthases5.3Genomic Analysis of pha Genes on Paraburkholderia and Burkholderia Species5.4Metabolic Routes of PHA Synthesis5.5PHA Production from Low-Cost Substrates5.6Properties of PHA Synthesized by Paraburkholderia and Burkholderia Species5.7Biomedical and Biotechnological ApplicationsReferences Chapter 6: Genetic Engineering as a Tool for Enhanced PHA Biosynthesis from Inexpensive Substrates6.1Introduction6.2Engineering Techniques Applied to Obtain Recombinant Strains for PHA Production6.3The Use of Whey as Carbon Source6.4The Use of Molasses as Carbon Source6.5The Use of Lipids as Carbon Source6.6The Use of Starchy Materials as Carbon Source6.7The Use of Lignocellulosic Materials as Carbon Source6.8Conclusions and OutlookReferences Chapter 7: Biosynthesis and Sequence Control of scl-PHA and mcl-PHA7.1Introduction7.2The Key Factors of PHA Biosynthesis7.3Sequence Control of scl-PHA and mcl-PHAReferences Chapters 8-15: Feedstocks Chapter 8: Inexpensive and Waste Raw Materials for PHA Production8.1Introduction8.2Oleaginous lipid-based feedstocks8.3Mixed Organic Acid Feedstocks8.4Mono- and Polysaccharide Feedstocks8.5Carbon Dioxide as a Feedstock8.6Other Carbon Feedstocks8.7Conclusions and OutlookReferences Chapter 9: Sustainable Production of Polyhydroxyalkanoates from Crude Glycerol9.1Introduction – Polyhydroxyalkanoates (PHA)9.2Crude Glycerol from Biodiesel Manufacture9.3Metabolic Pathways of PHA Synthesis from Glycerol9.4Production of PHA from Crude Glycerol9.5Characterization of PHA Synthesized from Glycerol9.6Metabolic Engineering for Glycerol-Based PHA Production9.7Impact of Crude Glycerol on the Molecular Mass of PHA9.8Conclusions and OutlookReferences Chapter 10: Biosynthesis of Polyhydroxyalkanoates (PHA) from Vegetable Oils and its By-products by Wild-Type and Recombinant Microbes10.1Introduction10.2Biosynthesis of PHA from Plant Oils10.3Challenges in Using Different Types of Microorganisms in Large Scale PHA Production10.4Application of Waste Vegetable Oils and Non-Food Grade Plant Oils for Large Scale Production of PHA10.5Conclusions and OutlookReferences Chapter 11: Production and Modification of PHA Polymers Produced from Long-Chain Fatty Acid11.1Introduction11.2Strategies for Production of mcl-PHA11.3Strategies for Maximum Volumetric Productivity11.4Strategies for Improved Substrate Yields from MCFAs and LCFAs11.5Extracellular Lipase for Triacylglyceride Consumption11.6Biosynthesis and Monomer Composition11.7Functional Modifications of mcl-PHA11.8Cross-Linking11.9Conclusions and OutlookReferences Chapter 12: Converting Petrochemical Plastic to Biodegradable Plastic12.1Introduction: The Plastic Waste Issue12.2Strategies for Up-Cycling of Plastic Waste12.3Enzymatic Degradation of Petrochemical Plastics12.4Metabolism of Plastics’ Monomers and the Connection with PHA12.5Conclusions and OutlookReferences Chapter 13: Comparing Heterotrophic with Phototrophic PHA Production - Concurring or Complementing Strategies?13.1Introduction – The Status Quo of PHB Production13.2Heterotrophic PHA Production for Comparison13.3PHB Synthesis in Cyanobacteria13.4Light as Energy Source for Cyanobacteria13.5CO2 as a Carbon Source for Cyanobacteria13.6Nutrients for Cyanobacterial Growth13.7Other Growth Conditions for Cyanobacteria13.8Current Status of Phototrophic PHA Production13.9Phototrophic Cultivation Systems13.10Recombinant Cyanobacteria for PHA Production13.11PHA Isolation from the Cells, Purification and Resulting Qualities13.12Utilisation of Residual Cyanobacteria Biomass13.13Comparing Heterotrophically with Phototrophically Produced PHB13.14Conclusions and OutlookReferences Chapter 14: Coupling Biogas (CH4) with PHA Biosynthesis14.1Introduction14.2Biogas Market14.3Methanotrophs14.4PHA Biosynthesis from Methane14.5Genome Scale Metabolic Models as a Tool for Understanding the Metabolism of PHB in Methanotrophs14.6Bioreactors for Biogas Bioconversion14.7Techno-Economic Analysis of PHA Production from BiogasReferences Chapter 15: Syngas as a Sustainable Carbon Source for PHA Production15.1Introduction15.2Syngas15.3Production of Syngas from Organic Waste and Biomass15.4Concept of Bacterial PHA Synthesis from Syngas15.5Production of PHA by Acetogens Based on Syngas as Substrate15.6PHA Production by Rhodospirillum rubrum Grown on Syngas15.7Synthesis of PHA by Carboxydobacteria Grown on Syngas15.8PHA Production by CO-Tolerant Hydrogen-Oxidizing Strains on Syngas15.9Bioprocesses for PHA Production on Syngas15.10Conclusions and OutlookReferences

O autorze

Martin Koller was awarded his PhD degree by Graz University of Technology, Austria, for his thesis on polyhydroxyalkanoate (PHA) production from dairy surplus streams which was enabled by the EU-project WHEYPOL (“Dairy industry waste as source for sustainable polymeric material production”), supervised by Gerhart Braunegg, one of the most eminent PHA pioneers. As senior researcher, he worked on bio-mediated PHA production, encompassing development of continuous and discontinuous fermentation processes, and novel downstream processing techniques for sustainable PHA recovery. His research focused on cost-efficient PHA production from surplus materials by bacteria and haloarchaea and, to a minor extent, to the development for PHA for biomedical use.
He currently holds more than 70 Web-of-science listed articles in high ranked scientific journals (h-index 23), authored twelve chapters in scientific books, edited three scientific books and four journal special issues on PHA, gave plenty of invited and plenary lectures at scientific conferences, and supports the editorial teams of several distinguished journals.
Moreover, Martin Koller coordinated the EU-FP7 project ANIMPOL (“Biotechnological conversion of carbon containing wastes for eco-efficient production of high added value products”), which, in close cooperation between academia and industry, investigated the conversion of animal processing industry´s waste streams towards structurally diversified PHA and follow-up products. In addition to PHA exploration, he was also active in microalgal research and in biotechnological production of various marketable compounds from renewables by yeasts, chlorophyte, bacteria, archaea, fungi or lactobacilli.
At the moment, Martin Koller is active as research manager and external supervisor for PHA-related projects.