The Dynamic Synapse: Molecular Methods in Ionotropic Receptor Biology

ISBN-13: 9780849318917 / Angielski / Twarda / 2006 / 336 str.

Stephen Moss; Josef Kittler; Kittler Kittler

Opis

Exploring the diverse tools and technologies used to study synaptic processes, The Dynamic Synapse: Molecular Methods in Ionotropic Receptor Biology delineates techniques, methods, and conceptual advances for studying neurotransmitter receptors and other synaptic proteins. It describes a broad range of molecular, biochemical, imaging, and electrophysiological approaches for studying the biology of synapses. Specific topics include the use of proteomics to study synaptic protein complexes, the development of phosphorylation state specific antibodies, post-genomic tools applied to the study of synapses and RNA interference in neurons. In addition, several chapters focus on methods for gene and protein delivery into neuronal tissue. The use of biochemical, electrophysiological and optical tagging techniques to study the movement and membrane trafficking of neurotransmitter receptors in the membrane of live nerve cells are also discussed. To complement these approaches, the application of approaches for achieving long-term alterations in the genetic complement of neurons in vivo using viral vectors or homologous recombination of ES cells are also described.

Spis treści

Methods for Uncovering the Mechanisms of AMPA Receptor Trafficking, S. Restituito and E.B. Ziff
Long-Term Plasticity at Inhibitory Synapses: A Phenomenon That Has Been Overlooked, J.-L. Gaiarsa and Y. Ben-Ari
New Tricks for an Old Dog: Proteomics of the PSD, B.A. Jordan, B.D. Fernholz, T.A. Neubert, and E.B. Ziff
Phosphorylation Site-Specific Antibodies as Research Tools in Studies of Native GABAA Receptors, J.N. Jovanovic
Protein Palmitoylation by DHHC Protein Family, Y. Fukata, D.S. Bredt, and M. Fukata
Studying the Localization, Surface Stability and Endocytosis of Neurotransmitter Receptors by Antibody Labeling and Biotinylation Approaches, I.L. Arancibia-Cárcamo, B.P. Fairfax, S.J. Moss, and J.T. Kittler
Visualization of AMPAR Trafficking and Surface Expression, P.V. Perestenko and J.M. Henley
Neurotransmitter Dynamics, S. Lévi and A. Triller
Receptor Dynamics at the Cell Surface Studied Using Functional Tagging, P. Thomas and T.G. Smart
RNAi and Applications in Neurobiology, T.C. Jacob
Transfecting and Transducing Neurons with Synthetic Nucleic Acids and Biologically Active Macromolecules, J.T. Kittler, J.G. Hanley, and J.T.R. Isaac
Acute In Vivo Expression of Recombinant Proteins in Rat Brain Using Sindbis Virus, H. Marie and R.C. Malenka
Lentivirus-Based Genetic Manipulations in Neurons In Vivo, P. Osten, T. Dittgen, and P. Licznerski
AMPA Receptor Phosphorylation in Synaptic Plasticity: Insights from Knockin Mice, H.-K. Lee
Genomic and Post-Genomic Tools for Studying Synapse Biology, J.T. Kittler and P.L. Oliver
Index

O autorze

Josef T. Kittler, Stephen J. Moss