Testicular Germ Cell Tumors: Methods and Protocols

ISBN-13: 9781071608623 / Angielski / Miękka / 2021 / 278 str.

Aditya Bagrodia; James F. Amatruda

Spis treści

Preface…

Table of Contents…

Contributing Authors…

1. Basic Histopathologic Assessment of Germ Cell Tumors for Clinic and Research

Payal Kapur and Dinesh Rakheja

2. Clinical Applications of Immunohistochemistry in Germ Cell Tumors in Men

Franto Francis and Ming Zhou

3. Molecular Characterization of Testicular Germ Cell Tumors Using Tissue Microdissection

Liang Cheng, Steven A. Mann, Antonio Lopez-Beltran, Michal Chovanec, Matteo Santoni, Mingsheng Wang, Costantine Albany, Nabil Adra, Darrell D. Davidson, Alessia Cimadamore, Rodolfo Montironi, and Shaobo Zhang

4. Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies in Testicular Germ Cell Tumors

Liang Cheng, Darrell D. Davidson, Rodolfo Montironi, Mingsheng Wang, Antonio Lopez-Beltran, Timothy A. Masterson, Costantine Albany, and Shaobo Zhang

5. Germ Cell Tumor Cell Culture Techniques 

John Lafin, James F. Amatruda, and Aditya Bagrodia

6. Three-Dimensional Cultivation of Germ Cell Cancer Cell Lines as Hanging Drops

Margaretha A. Skowron, Meike M. Watolla, and Daniel Nettersheim

7. Cultivation of Testicular Germ Cell Cancer Cell Lines and Establishment of Gene-Edited Subclones using CRISPR/Cas9

Sina Jostes, Daniel Nettersheim, Simon Schneider, and Hubert Schorle

8. Evaluation of Chemotherapeutic Drugs for Treatment of (Cisplatin-Resistant) Germ Cell Cancer Cell Lines

Margaretha A. Skowron, Michèle J. Hoffmann, Meike M. Watolla, and Daniel Nettersheim

9. Assessing Homologous Recombination and Interstrand Crosslink Repair in Embryonal Carcinoma Testicular Germ Cell Tumor Cell Lines

Francesca Cavallo, Cinzia Caggiano, Maria Jasin, and Marco Barchi

10. Production and Analysis of Human Primordial Germ Cell-Like Cells

Shino Mitsunaga, Keiko Shioda, Jacob H. Hanna, Kurt J. Isselbacher, and Toshi Shioda

11. A Genetically Engineered Mouse Model of Malignant Testicular Germ Cell Tumors

Amy M. Lyndaker, Timothy M. Pierpont, Amanda R. Loehr, and Robert S. Weiss

12. Targeted Methylation Analyses: From Bisulfite Treatment to Quantification 

João Lobo, Ad J. M. Gillis, and Leendert H. J. Looijenga 

13. Integrated Analysis of Germ Cell Tumors

Alok Tewari and Eliezer Van Allen

14. Use of Genome-Wide Association Studies to Evaluate Genetic Predisposition to Testicular Germ Cell Tumors

Anthony J. Hooten, Erica Langer, and Jenny N. Poynter

15. A Circulating MicroRNA Panel for Malignant Germ Cell Tumor Diagnosis and Monitoring

Matthew J. Murray, Cinzia G. Scarpini, and Nicholas Coleman

16. Detection of Circulating Tumor Cells (CTCs) in Patients with Testicular Germ Cell Tumors 

Paulina Nastały, Friedemann Honecker, Klaus Pantel, and Sabine Riethdorf

17. Developing and Using a Data Commons for Understanding the Molecular Characteristics of Germ Cell Tumors

Bo Ci, Shin-Yi Lin, Bo Yao, Danni Luo, Lin Xu, Mark Krailo, Matthew Murray, James F. Amatruda, A. Lindsay Frazier, and Yang Xie

Subject Index List…

Blurb

This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology; in vitro and in vivo techniques for investigating GCT biology; novel methods for detecting minimal residual diseases; and sequencing considerations as they apply to GCTs. Chapters cover topics such as clinical applications of immunohistochemistry in GCTs in men; fluorescence in situ hybridization (FISH) detection of chromosomal 12p anomalies in testicular GCTs; evaluation of chemotherapeutic drugs for treatment of (cisplatin-resistant) germ cell cancer cell lines; targeted methylation analyses; and circulating microRNA panel for malignant GCT diagnosis and monitoring. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Testicular Germ Cell Tumors: Methods and Protocols provides valuable information that is useful for both novice and expert GCT researchers interested in learning more about this field.