Tertiary Lymphoid Structures: Methods and Protocols

ISBN-13: 9781493987085 / Angielski / Twarda / 2018 / 289 str.

Marie-Caroline Dieu-Nosjean

Opis

This volume explores the various methods used to study tertiary lymphoid structures (TLS) in pathological situations.

Spis treści

Preface…

Table of Contents…

Contributing Authors…

1. Tertiary Lymphoid Structures among the World of Non-Canonical Ectopic Lymphoid Organizations

Aaron Silva-Sanchez, Troy D. Randall, and Selene Meza Perez

2. Cellular and Vascular Components of Tertiary Lymphoid Structures

Chistopher Georges Mueller, Saba Nayar, David Gardner, and Francesca Barone

3. Meningeal Immunity, Drainage, and Tertiary Lymphoid Structure Formation

Antoine Louveau

4. Development of Tools for the Selective Visualization and Quantification of TLS-Immune Cells on Tissue Sections

Christophe Klein, Priyanka Devi-Marulkar, Marie-Caroline Dieu-Nosjean, and Claire Germain

5. A Quantitative Pathology Approach to Analyze the Development of Human Cancer-Associated Tertiary Lymphoid Structures

Karīna Siliņa, Chiara Burkhardt, Ruben Casanova, Alex Solterman, and Maries van den Broek

6. Multiplex Immunohistochemistry for Image Analysis of Tertiary Lymphoid Structures in Cancer

Keith E. Steele and Charles Brown

7. Defining High Endothelial Venules and Tertiary Lymphoid Structures in Cancer Emma Jones, Awen Gallimore, and Ann Ager

8. Development of Methods for Selective Gene Expression Profiling in Tertiary Lymphoid Structure using Laser Capture Microdissection

Claudia Gutierrez-Chavez, Samantha Knockaert, Marie-Caroline Dieu-Nosjean, and Jérémy Goc

9. Quantifying Tertiary Lymphoid Structure-Associated Genes in Formalin-Fixed Paraffin-Embedded Breast Cancer Tissues

Chunyan Gu-Trantien, Soizic Garaud, Edoardo Migliori, Cinzia Solinas, Jean-Nicolas Lodewyckx, and Karen Willard-Gallo

10. Generation of Recombinant Monoclonal Antibodies from Single B Cells Isolated from Synovial Tissue of Rheumatoid Arthritis Patients

Elisa Corsiero, Lucas Jagemann, Michele Bombardieri, and Costantino Pitzalis

11. Designed Methods for the Sorting of Tertiary Lymphoid Structure-Immune Cell Populations

Priyanka Devi-Marulkar, Hélène Kaplon, Marie-Caroline Dieu-Nosjean, and Myriam Lawand

12. Identification of Tertiary Lymphoid Structure-Associated Follicular Helper T Cells in Human Tumors and Tissues

Coline Couillault, Claire Germain, Bertrand Dubois, and Hélène Kaplon

13. Exploring the Role of Tertiary Lymphoid Structures using a Mouse Model of Bacteria-Infected Lungs

Jean-Luc Teillaud, Lucile Regard, Clémence Martin, Sophie Sibéril, and Pierre-Régis Burgel

14. Identification and Characterization of Tertiary Lymphoid Structures in Murine Melanoma

Anthony B. Rodriguez, J. David Peske, and Victor H. Engelhard

15. Investigating Tumor-Associated Tertiary Lymphoid Structures in Murine Lung Adenocarcinoma

Kelli A. Connolly, Mursal Nader, and Nikhil Joshi

16. Targeting Tertiary Lymphoid structures for Tumor Immunotherapy

Haidong Tang, Xiangyan Qiu, Casey Timmerman, and Yang-Xin Fu

Blurb

This volume explores the various methods used to study tertiary lymphoid structures (TLS) in pathological situations. Pre-clinical models are also discussed in detail to show how TLS structure, development, and maintenance can be targeted and studied in vivo. The chapters in this book cover topics such as humans and mice; strategies to quantify TLS in order to use it in stained tissue sections; classifying a gene signature form fixed and paraffin-embedded tissues; and development of murine inflammatory models to help look at TLS in the context of infection or malignancy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Tertiary Lymphoid Structures: Methods and Protocols is a valuable resource that increases the reader’s knowledge on immune functions and how they will pave the way to future therapeutic applications.