Teratogenicity Testing: Methods and Protocols

ISBN-13: 9781493993147 / Angielski / Miękka / 2019 / 614 str.

Félix, Luís

Opis

This detailed book provides a compilation of laboratory techniques and tests to assess the risks to embryo-fetal development from drug exposure during early developmental stages. After an introduction to teratogenicity testing, the contents explore both in vitro and in vivo techniques in the study of the teratogenic and fetotoxic effects of drugs, as well as numerous animal-based teratology methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Teratogenicity Testing: Methods and Protocols describes methods that integrate a battery of tests that can be performed in cells, organs, tissues, and animal models for evaluating toxicity and/or the safety of compounds in early developmental stages with the goal of estimating, preventing, or minimizing the teratogenic potential of drugs.

Spis treści

Part I: Introduction to Teratology Testing

 

1. An Overview of Teratology

            Ana M. Calado and Maria dos Anjos Pires

 

2. Teratology Study Guidelines: An Overview

            Sofia Alves-Pimenta, Luís Félix, Bruno Colaço, Paula A. Oliveira, and Carlos Venâncio

 

3. Biological Concerns on the Selection of Animal Models for Teratogenic Testing

            Sofia Alves-Pimenta, Bruno Colaço, Paula A. Oliveira, and Carlos Venâncio

 

Part II: In Vitro-Based Teratology Screening

 

4. The Validated Embryonic Stem Cell Test with Murine Embryonic Stem Cells

            Lauren M. Walker, Darcie L.V. McClelland-Descalzo, and Nicole I. zur Nieden

 

5. Human Pluripotent Stem Cells to Assess Developmental Toxicity in the Osteogenic Lineage

            Joseph V. Madrid, Steven R. Sera, Nicole R.L. Sparks, and Nicole I. zur Nieden

 

6. Chick Embryonic Cardiomyocytes Micromass System for Assessing Developmental Cardiotoxicity of Drugs

            Wasay M.S. Qureshi and Margaret K. Pratten

 

7. Flow Cytometry to Evaluate Potential Developmental Toxicants in the Embryonic Stem Cell

            Valéria Maria Lara, Kelly C. Santos Roballo, André Tadeu Gotardo, and Carlos Eduardo Ambrósio

 

8. Morphology-Based Whole Embryo Culture for Developmental Toxicity of Drugs

            Bo Liang and Chi C. Wang

 

9. Western Blot Methodologies for Analysis of In Vitro Protein Expression Induced by Teratogenic Agents

            Carlos Martins-Gomes and Amélia M. Silva

 

10. Manipulation of MicroRNAs in Cultured Mouse Embryos: Applications for Developmental Toxicology

            Fuqiang Yuan and Shao-yu Chen

 

Part III: Animal-Based Teratology Methods

 

11. Insights into the Phenotypic and Behavioral Effects of Teratogenic Drugs in Caenorhabditis elegans

            Caralina M. de Evsikova

 

12. Effect of Teratogens on Development of Drosophila melanogaster

            Dong Li and Xiaolin Bi

 

13. Cellular Responses in Drosophila melanogaster Following Teratogen Exposure

            Matheus C. Bianchini, José L.R. Portela, Robson L. Puntel, and Daiana S. Ávila

 

14. Behavioral Teratogenesis in Drosophila melanogaster

            Monalisa Mishra and Bedanta Kumar Barik

 

15. Evaluation of the Teratogenicity of Pharmaceuticals Using FETAX

            Hariz Islas-Flores, Itzayana Pérez-Alvaréz, and Leobardo M. Gómez-Oliván

 

16. Histological Observation of Teratogenic Phenotypes Induced in Frog Embryo Assays

            Mark E. Pownall and Margaret S. Saha

 

17. Visualization of Gene Expression Patterns by In Situ Hybridization on Early Stages of Development of Xenopus laevis

            Heithem M. El-Hodiri and Lisa E. Kelly

 

18. Analysis of Lethality and Malformations During Zebrafish (Danio rerio) Development

            Azhwar Raghunath and Ekambaram Perumal

 

19. General Whole-Mount Immunohistochemistry of Zebrafish (Danio rerio) Embryos and Larvae Protocol

            Dércia Santos, Sandra M. Monteiro, and Ana Luzio

 

20. Geometric Morphometrics as a Tool to Evaluate Teratogenic Effects in Zebrafish (Danio rerio)

            Kathryn E. Perez and Tisha C. King-Heiden

 

21. Live Metabolic Profile Analysis of Zebrafish Embryos Using a Seahorse XF 24 Extracellular Flux Analyzer

            Simon T. Bond, Kevin A. McEwen, Prusothman Yoganantharajah, and Yann Gibert

 

22. Behavioral Profiling of Zebrafish (Danio rerio) Larvae Following Teratogen Exposure

            Ana M. Valentim

 

23. Omics in Zebrafish Teratogenesis

            Benjamin Piña, Laia Navarro, Carlos Barata, Demetrio Raldúa, Rubén Martínez, and Marta Casado

 

24. Proteomic Analysis of Zebrafish (Danio rerio) after Chemical Exposure

            Yew Mun Lee, Caixia Li, Siew Hong Lam, Zhiyuan Gong, and Qingsong Lin

 

25. Immunohistochemical Assessment as a Tool for Investigating Developmental Toxicity in Zebrafish (Danio rerio)

            Carla Santos and Maria de Lurdes Pinto

 

26. Oxidative Stress Assessment in Zebrafish Larvae

            Inês Domingues and Carlos Gravato

 

27. Hemodynamic Studies for Analyzing the Teratogenic Effects of Drugs in the Zebrafish Embryo

            Huseyin C. Yalcin

 

28. Western Blot Analysis and Immunostaining for Prediction of Embryotoxicity in Mus musculus

            Yulia Irnidayanti, Win Darmanto, and Dias R. Sutiono

 

29. Histological and Histochemical Profile for Teratological Assessment in Mus musculus

            Maria de Lurdes Pinto and Paula Rodrigues

 

30. In Vivo Analysis of Apoptosis in Embryonic Hippocampus

            Ahmad Salimi and Jalal Pourahmad

 

31. Measurement of Mitochondrial Toxicity Parameters in Embryonic Hippocampus

            Ahmad Salimi and Jalal Pourahmad

 

32. Animal Tests for Evaluation of Cognitive Impairment in Neonatal Mouse

            Ahmad Salimi and Jalal Pourahmad

 

33. Methodology of Genotoxic and Teratogenic Studies in Rats

            Eyyup Rencuzogullari and Muhsin Aydin

 

34. Whole Mount In Situ Hybridization and Morphometric Analysis in Rabbit Embryos

            Yoshinori Kawamura and Kiyoshi Matsumoto

 

Glossary of Key Terms for Teratology

            Maria dos Anjos Pires and Ana M. Calado

Index