Techniques in Neuroanatomical Research

ISBN-13: 9783642680311 / Angielski / Miękka / 2011 / 398 str.

C. Heym; W. -G Forssmann

Opis

"Anatomy is the mother of physiology" - this statement was used to characterize the evolution of physiology from anato my as an independent science in the late nineteenth century. It had particular truth for neurophysiology, which started as functional neuroanatomy based on the observation of changes in behaviour after lesions of the nervous system both in ex perimental animals and in human patients. Today, anatomy may again be considered the mother of physiology; however, the meaning of this statement is rather different from that 100 years ago: The modem mother provides a dwelling for an increasing number of children endowed with new functional capabilities. This book provides a good illustration of such se mantic metamorphosis in the case of neuroanatomy. After a long period of little progress in either macroscopic neuroanatomy or neurohistology, during which the heritage of Cajal, Golgi, and others was developed and refined to yield a functional concept of the nervous system, the past two de cades have seen tremendous progress in methods applicable to the analysis of the nervous system. The new era was heralded by the introduction of the electron microscope to investigate the nervous system. This book is an impressive witness to the more recent developments."

Spis treści

I General Research Methods in Neuroanatomy.- 1 General Methods in Light Microscopy of the Nervous System.- 2 General Methods in Transmission Electron Microscopy of the Nervous System..- 3 Freeze-Etching in Neuroanatomy..- 4 General Methods in Scanning Electron Microscopy of the Nervous System.- 5 Lesion Methods in Neurobiology..- 6 General Methods for Characterization of Brain Regions..- II Light Microscopical Research Methods in Neuroanatomy.- 7 Enzyme Histochemistry of Nervous Tissue..- 8 The Golgi Methods..- 9 Fluorescence Histochemistry of Biogenic Monoamines..- 10 Immunohistochemistry and Immunocytochemistry of Nervous Tissue..- 11 Identification of Single Neurons by Intracellular Application of Tracers..- 12 Light Microscopical Autoradiography of Nervous Tissue..- 13 Brain Localization of Hormones and Drugs by Thaw-mount Autoradiography, Combined Autoradiography-Formaldehyde Induced Fluorescence, and Combined Autoradiography-Immunohistochemistry..- 14 Combined Immunocytochemistry and Autoradiography: In Vivo Injections of Monoclonal Antibodies and Radioactive Amines (Substance P and 3H-Serotonin).- III Electron Microscopical Research Methods in Neuroanatomy.- 15 Quick-Freezing Methods in Neuroanatomy..- 16 Ultrastructural Histochemistry of Nervous Tissue..- 17 The Zinc Iodide-Osmium Tetroxide (ZIO) Method..- 18 Combined Freeze-Fracturing and Autoradiography Techniques: Freeze-Fracture Autoradiography..- IV Investigation of Living Nervous Tissue.- 19 Extracellular Marking and Retrograde Labelling of Neurons..- 20 Cell, Tissue, and Organ Culture in Neuroanatomy..