Technical Aspects of Toxicological Immunohistochemistry: System Specific Biomarkers

ISBN-13: 9781493915156 / Angielski / Twarda / 2015 / 231 str.

Syed A. Aziz; Rekha Mehta

Opis

This authoritative volume examines immunohistochemical methods aimed at investigating the toxicologic pathology of rodent, non-human primate and aquatic animal tissues. Eleven comprehensive chapters provide pathologists and researchers in various sub-disciplines of toxicology with a comprehensive review of the methods and approaches for immunohistochemical staining in various target tissues. It explores the tissue-antigen and antibody-specific problems that may be encountered during the staining procedures and provide potential avenues for resolving various methodological issues. Special attention is paid to the latest enhancement procedures for antigen retrieval and visualization as well as image analysis and antigen quantification. Written by leading researchers in toxicology and pathology, this book is a significant resource for toxicologists and pathologists working with rodents, monkeys and aquatic animal tissues.

Spis treści

1.Immunohistochemistry – Overview, Its Potential and Challenges.- 2.Tissue Micro Array (TMA) Technology and its Potential Applications in Toxicology and Toxicological Immunohistochemistry.- 3.Importance of Fluorescent In Situ Hybridization (FISH) in Rodent Tumors.- 4.Quantitation of Immunohistochemistry by Image Analysis Technique.- 5.Immunohistochemistry in the Study of Cancer Biomarkers for Oncology Drug Development.- 6.Male Reproductive Toxicology and the Role of Immunohistochemistry.- 7. Immunohistochemistry and Female Reproductive Toxicology: The Ovary and Mammary Gland.- 8.Immunohistochemistry on Rodent Circulatory System: Its Possible Use in Investigating Hypertension.- 9.Role of Lymphoid/Mucosa-Associated Lymphoid Tissue Markers in Toxicological Immunohistochemistry. 10.Bone Markers and Immunohistochemistry Changes in Toxic Environments.- 11. Biomarkers of Skeletal Muscle Injury in the Rat.

O autorze

Syed A Aziz, Ph.D.

Regulatory Toxicology Research Division, Bureau of Chemical Safety

Food Directorate, Health Products and Food Branch

Health Canada

Adjunct professor, Faculty of Medicine Dept. of Pathology

University of Ottawa

Rekha Mehta, Ph.D.

Regulatory Toxicology Research Division, Bureau of Chemical Safety

Food Directorate, Health Products and Food Branch

Health Canada.

Blurb

This volume examines immunohistochemical methods for investigating the toxicologic pathology of rodent and other animal tissues. Eleven comprehensive chapters provide pathologists and researchers in various sub-disciplines of toxicology with an authoritative review of the methods of immunohistochemical staining in various target tissues. Further, this book explores the tissue-, antigen-, and antibody-specific problems that may be encountered during the staining procedures and provides potential avenues for resolving various methodological issues. Special attention is paid to the latest enhancement procedures for antigen retrieval and visualization as well as to image analysis and antigen quantification. Written by leading researchers in toxicology and pathology, this book is a significant resource for toxicologists and pathologists working with rodents and other animal models. The information in Technical Aspects of Toxicological Immunohistochemistry is rounded out by the companion book, Toxicological Immunohistochemistry which examines mechanistic and predictive biomarkers for toxicological evaluation.