TISSUE CULTURE STUDIES IN CHICK PEA (CICER ARIETINUM.L.) GRAIN LEGUME

ISBN-13: 9786206146179 / Angielski / Miękka / 144 str.

Uppu Anitha Devi; Thirunahari Ugandhar

Opis

Chickpeas are well-known as a good source of protein. Chickpea area and productivity are declining because to its sensitivity to insect pests, fungal diseases, and limited drought tolerance. The current study aims to create methods for in vitro regeneration and in vitro rooting hardness of chickpea cv. ICCC-34 kranthi. In the ICCC-34 Kranthi genotype, callus induction and in vitro regeneration were attempted. The study employed MS medium supplemented with numerous growth regulators in varied combinations and quantities. In the ICCC 34 kranthi genotype, the number of days required for callus induction ranged from 6.0 to 10.0 days. The average number of days required for hypocotyls was 7.9 days and 8.2 days for leaf explants. The callus induction response differed significantly between genotypes, explants, medium, and their interaction impact. In direct regeneration studies, a mature embryo axe with a single cotyledon outperformed the other two with the highest shoot induction response (84.17%) regardless of medium. TDZ at 0.5 mg/l not only caused shooting in more explants (87.50%) but also recorded the most multiple shoots to separate them individually.

O autorze

Dr. U. Anitha Devi received her doctorate from Kakatiya University at the age of 25 and was appointed by the APPSC to the Government College for Women at Karimnagar. She has published 44 research articles and co-authored three books. 34 national seminars, three international seminars, four symposia, and workshops were attended.