T Follicular Helper Cells: Methods and Protocols

ISBN-13: 9781493955930 / Angielski / Miękka / 2016 / 228 str.

Marion Espeli; Michelle Linterman

Spis treści

Part I: Mouse

1. Identification of Mouse T Follicular Helper Cells by Flow Cytometry

Alexandre Meli & Irah King

 2. Identification of Follicular T Helper Cells in Tissue Sections

Yanna Ding, John D. Mountz, and Hui-Chen Hsu

 3. Tracking Early T Follicular Helper Cell Differentiation In vivo

Dirk Baumjohann and K. Mark Ansel

 4. Tracking by Flow Cytometry Antigen-Specific Follicular Helper T-Cells In Wild-Type Animals After Protein Vaccination

Svetoslav Chakarov and Nicolas Fazilleau

 5. Retroviral Vector Expression in TCR Transgenic CD4⁺ T Cells

Youn Soo Choi and Shane Crotty

 6. Two-photon Microscopy For Imaging Germinal Centres and T Follicular Helper Cells

Menna R. Clatworthy

7. In vivo Induction of T-follicular Helper Cells by Modulation of Regulatory T Cell Function

James B. Wing and Shimon Sakaguchi

 8. Assessing T Follicular Helper Cell Function in vivo: Antigen-specific B Cell Response to Hapten and Affinity Maturation

Jessica Natt and Marion Espéli

 9. The SW_HEL System For High-resolution Analysis of in vivo Antigen-specific T-Dependent B Cell Responses

R. Brink, D. Paus, K. Bourne, J.R. Hermes, S. Gardam, T.G. Phan, and T.D. Chan

 10. Triggering Positive Selection of Germinal Center B cells by Antigen Targeting to DEC-205

Giulia Pasqual, Alessandro Angelini, and Gabriel D. Victora

 11. Detection of Mouse Natural Killer T Follicular Helper (NKT_FH) Cells by Flow Cytometry

Pheh-Ping Chang and Carola G. Vinuesa

 12. Identification of Foxp3⁺ T Follicular Regulatory (Tfr) Cells

by Flow Cytometry

 Ana Raquel Maceiras and Luis Graca

 13. In vitro Assay to Sensitively Measure Tfr Suppressive Capacity and Tfh Stimulation of B Cell Responses

Peter T. Sage and Arlene H. Sharpe

 Part II: Human

14. Flow Cytometric Detection and Isolation of Human Tonsil or Lymph Node T Follicular Helper Cells

Jan Misiak, Karin Tarte, and Patricia Amé-Thomas

15. Human Tfh and Tfr cells: Identification and Assessment of their Migration Potential

Chang H. Kim, Seika Hashimoto-Hill, and Seung G. Kang

 16. Analysis of Human Blood Memory T Follicular Helper Subsets

Salah-Eddine Bentebibel, Clement Jacquemin, Nathalie Schmitt, and Hideki Ueno

 17. Flow Cytometric Analysis of Circulating Follicular Helper T (Tfh) and Follicular Regulatory T (Tfr) Populations in Human Blood

Yunbo Wei, Jinghong Feng, Zhaohua Hou, Xin Maggie Wang, and Di Yu

 18. Quantifying Helper Cell Function of Human TFH Cells in vitro

Nina Chevalier

Blurb

This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans.  As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward.