T. Cruzi Infection: Methods and Protocols

ISBN-13: 9781493991471 / Angielski / Twarda / 2019 / 398 str.

Karina Andrea Gomez; Carlos Andres Buscaglia

Recenzje

"The book is written for students, active researchers, and clinicians. ... This book comprehensively covers the experimental methods for the study of the biology of T. cruzi and its complex interactions with the human host. The unique feature of the book is that the variety of methods and protocols it covers. There are very few books that provide the depth of coverage this book does." (Omer Iqbal, Doody's Book Reviews, August 2019)

Spis treści

Preface…

Table of Contents…

Contributing Authors…

1. Trypanosoma cruzi Genome Assemblies: Challenges and Milestones of Assembling a Highly Repetitive and Complex Genome

João Luís Reis-Cunha and Daniella C. Bartholomeu

2. Mining Trypanosoma cruzi Genome Sequences for Antigen Discovery and Vaccine Development

Christian Teh-Poot and Eric Dumonteil

3. Transcriptome Studies in Trypanosoma cruzi using RNAseq

Gonzalo Greif, Luisa Berná, Florencia Díaz-Viraqué, and Carlos Robello

4. An Improved Approach to Trypanosoma cruzi Molecular Genotyping by Next-Generation Sequencing of the Mini-Exon Gene

Alicia Majeau, Claudia Herrera, and Eric Dumonteil

5. Genome Editing by CRISPR/Cas9 in Trypanosoma cruzi

Noelia Lander, Miguel A. Chiurillo, and Roberto Docampo

6. Sample Preparation of Trypanosoma cruzi Surface, Extracellular, and Nuclear Subproteomes 

Carlos André Ornelas Ricart, Rayner Myr Lauterjung Queiroz, Agenor de Castro Moreira dos Santos Júnior, and Sébastien Charneau

7. Isolation and Characterization of Extracellular Vesicles Derived from Trypanosoma cruzi

Izadora Volpato Rossi, Bruno Gavinho, and Marcel Ivan Ramirez

8. Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae   

María José Ferrer, Diana Patricia Wehrendt, Mariana Bonilla, Marcelo Alberto Comini, María Teresa Tellez-Iñón, and Mariana Potenza

  

9. Homologous Expression of Glycosylphosphatidyl Inositol-Anchored Glycoproteins in Trypanosoma cruzi 

Virginia Balouz, Andrea C. Mesias, Camila Centeno Camean, Ivana Ducrey, Maite Mabel Lobo, Ignacio M. Durante, Gaspar E. Cánepa, Carlos A. Buscaglia, and María de los Milagros Cámara

10. Metabolic Labeling of Surface Neo-sialylglyconjugates Catalyzed by Trypanosoma cruzi trans-Sialidase

Giannina Carlevaro, Andrés B. Lantos, Gaspar E. Cánepa, María de los Milagros Cámara, Martín Somoza, Carlos A. Buscaglia, Oscar Campetella, and Juan Mucci

11. Exploiting Genetically Modified Dual-Reporter Strains to Monitor Experimental Trypanosoma cruzi Infections and Host: Parasite Interactions 

Martin C. Taylor, Amanda F. Francisco, Shiromani Jayawardhana, Gurdip Singh Mann, Alexander Ward, Francisco Olmo, Michael D. Lewis, and John M. Kelly

12. Transmigration of Trypanosoma cruzi trypomastigotes through 3D Spheroids Mimicking Host Tissues

Matías Exequiel Rodríguez, Mariana Rizzi, Lucas Caeiro, Yamil Masip, Daniel O. Sánchez, and Valeria Tekiel

13. In Situ Detection of Dormant Trypanosoma cruzi Amastigotes using Bioluminescent-Fluorescent Reporters

Fernando Sánchez-Valdéz and Angel Padilla 

14. Multi-Parametric Evaluation of Trypanosoma cruzi Infection Outcome in Animal 

Models

Julien Santi-Rocca, Núria Gironès, and Manuel Fresno

15. MicroRNA Transcriptome Profiling in Heart of Trypanosoma cruzi-Infected Mice

Ludmila Rodrigues Pinto Ferreira

16. Genotyping of Trypanosoma cruzi from Clinical Samples by Multilocus Conventional PCR 

Otacilio C. Moreira and Juan Carlos Ramirez

17. Assessing Parasite Load in Chagas Disease Patients by Quantitative Multiplex Real-Time PCR

Juan Carlos Ramírez and Otacilio da Cruz Moreira

18. Diagnostic Applicability of Neutralizing Antibodies to Trypanosoma cruzi trans-sialidase 

Leguizamón María Susana

19. Analytics of Functional Autoantibodies in Patients with Chagas’ Disease

Gerd Wallukat, Katrin Wenzel, and Ingolf Schimke

20. Apolipoprotein-A1 and Fibronectin Fragments as Markers of Cure for the Chagas Disease

Elizabeth Ruiz-Lancheros, Makan Golizeh, and Momar Ndao 

21. Host Derived Molecules as Novel Chagas Disease Biomarkers: Hypercoagulability Markers in Plasma

Julio Alonso-Padilla, Dolors Tassies, Nuria Cortes-Serra, Joaquim Gascon, Joan-Carles Reverter, and María-Jesús Pinazo

22. Purification of GlycosylphosphatidyLinositol-Anchored Mucins from Trypanosoma cruzi Trypomastigotes and Synthesis of -Gal-Containing Neoglycoproteins: Application as Biomarkers for Reliable Diagnosis and Early Assessment of Chemotherapeutic Outcomes of Chagas Disease

Uriel Ortega-Rodriguez, Susana Portillo, Roger A. Ashmus, Jerry A. Duran, Nathaniel S. Schocker, Eva Iniguez , Alba L. Montoya, Brenda G. Zepeda, Janet J. Olivas, Nasim H. Karimi, Julio Alonso-Padilla, Luis Izquierdo, Maria-Jesús Pinazo, Belkisyolé Alarcón de Noya , Oscar Noya, Rosa A. Maldonado, Faustino Torrico, Joaquim Gascón , Katja Michael, and Igor C. Almeida 

23. Cytometric Bead Array (CBA) for Measurement Cytokines Levels in Chagas Disease Patients

Nayara I. Medeiros and Juliana A. S. Gomes

24. Generation of T. cruzi-specific Primary CD4+ T Cell Lines from Peripheral Blood Mononuclear Cells Isolated from Chagas Disease Patients 

Gonzalo R. Acevedo, Paula B. Alcaráz, Clemencia Pinilla, and Karina A. Gómez

25. Trypanosma cruzi Specific T-Cell Responses to Monitor Treatment Efficacy in Chronic Chagas Disease

María Cecilia Albareda, María Ailén Natale, Gonzalo Leandro Cesar, Melisa Daiana Castro Eiro, María Gabriela Alvarez, and Susana Adriana Laucella

26. CD8+ T Cell Response to Trypanosoma cruzi Antigens during Chronic Chagas Disease

Paola Lasso, Jose Mateus, John Mario González, Adriana Cuéllar, and Concepción Puerta

27. Fluorescence Proteomic Technology to Analyze Peripheral Blood Mononuclear Cells in Chronic Chagas Disease 

John E. Wiktorowicz, M. Paola Zago, and Nisha J. Garg

28. Isolation and Phenotypic Characterization of Inflammatory Cells from Clinical Samples: Purification of Macrophages from Trypanosoma cruzi-Infected Hearts

Natalia Eberhardt, Liliana María Sanmarco, and Maria Pilar Aoki

Blurb

This volume provides detailed descriptions on cutting-edge experimental methods to assess the multiple and complex interactions between T. cruzi and its host. Chapters detail a series of methods and protocols, ranging from rather classical biochemical/genetic approaches to modern, powerful and high-throughput ‘-omics’ technologies, to tackle this issue, as well as novel techniques to improve treatment and clinical evaluation of Chagasic patients. Genome-wide mining strategies aimed at identifying potential parasite antigens and vaccine candidates and an overview on the challenges and milestones encountered during T. cruzi genome assembly are also included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, T. cruzi Infection: Methods and Protocols aims to provide students, researchers, and clinicians who are interested in the study of Chagas disease an indispensable source of information.