T-Cell Trafficking: Methods and Protocols

ISBN-13: 9781493957040 / Angielski / Miękka / 2017 / 290 str.

Federica M. Marelli-Berg; Sussan Nourshargh

Opis

Recent discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. This book explores how the development of novel techniques has led to an increased understanding of lymphocyte trafficking.

Recenzje

From the reviews:

"This book, as the title describes, is primarily a collection of methods to investigate T-cell trafficking. ... this book details a comprehensive range of techniques for monitoring T-cell trafficking, along with the background behind the techniques, as well as their potential uses. This book would appeal to anyone interested in the field of T-cell trafficking and would be invaluable to PhD students and postdoctoral scientist setting up these techniques." (Judith Ramage, Immunology News, August, 2011)

Spis treści

T-Cell Trafficking: Methods and Protocols Edited by Federica M. Marelli-Berg and Sussan Nourshargh Table of Contents Preface Contributors Introductory review: 1. How T cells find their way around Alf Hamann Migration of T cells in vitro 2. Live imaging of leukocyte-endothelium interactions Olga Barreiro, Francisco Sánchez-Madrid, and María Yánez-Mó 3. Leukocyte adhesion under haemodynamic flow conditions Charlotte Lawson, Marlene Rose and Sabine Wolf 4. Influence of stromal cells on lymphocyte adhesion and migration on endothelial cells Helen M. McGettrick PhD, Chris D. Buckley PhD, G. Ed Rainger PhD and Gerard B. Nash PhD 5. Discriminating between the paracellular and transcellular routes of diapedesis Eva Cernuda-Morollón, Severine Gharbi, Jaime Millán 6. Monitoring RhoGTPase activity in lymphocytes Marouan Zarrouk, David Killock, Aleksandar Ivetic 7. Visualisation of Signalling in Immune Cells Leo M. Carlin, Konstantina Makrogianneli, Melanie Keppler, Gilbert O. Fruhwirth and Tony Ng 8. Methods for quantisation of lymphocyte chemotaxis and fugetaxis Fabrizio Vianello, Elda Righi and Mark C. Poznansky 9. Analysis of CXCR3 and Atypical Variant Expression and Signalling in Human T Lymphocytes Anna Korniejewska, Malcolm Watson, Stephen Ward 10. Transfection of indoleamine 2,3 dioxygenase in primary endothelial cells Petros XE Mouratidis, Andrew J T George Migration of T cells in vivo 11. Visualisation of lymphoid organ development Henrique Veiga-Fernandes, Katie Foster, Amisha Patel, Mark Coles, and Dimitris Kioussis 12. Single Cell Analysis of Cytotoxic T Cell Function by Intravital Multiphoton Microscopy Thorsten R. Mempel 13. Imaging interactions between the immune and cardiovascular systems in vivo by multiphoton microscopy Owain R. Millington, James M. Brewer, Paul Garside, and Pasquale Maffia 14. Applying an adaptive watershed to the tissue cell quantification during T-cell migration and embryonic development D. Zhu, S. Jarmin, A. Ribeiro, F. Prin, S.Q. Xie, K. Sullivan, J. Briscoe, A.P. Gould, F.M. Marelli-Berg & Y. Gu Monitoring T cell migration in human diseases 15. Identifying homing interactions in T cell traffic in human disease Patricia F. Lalor, Stuart M. Curbishley and David H. Adams 16. Tracking antigen experienced effector T cells in vitro and in vivo Claire L. Gorman, Claudia Monaco, Enrico Ammiratti, Anna-Chiara Vermi, Federica Marelli-Berg and Andrew P. Cope 17. Pre-clinical testing of strategies for therapeutic targeting of human T cell trafficking in vivo Caroline Coisne and Britta Engelhardt

Blurb

In the last decade, a large number of major discoveries have shed light on the molecular mechanisms of lymphocyte migration and the anatomy of immune responses. In T-Cell Trafficking: Methods and Protocols, expert researchers explore how the development of novel and cutting-edge techniques, particularly in the field of real-time imaging and genetic manipulation, have led to an increased understanding of lymphocyte trafficking. Written by internationally recognized experts in their respective fields, chapters provide state-of-the-art protocols to study lymphocyte migration and T-cell: endothelial cell interactions in vitro, address various approaches used for direct visualization of the development of the lymphoid system, lymphocyte recirculation, and effector responses in experimental models in vivo, and explore lymphocyte migration and inflammation in the human system. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.



Innovative and highly practical, T-Cell Trafficking: Methods and Protocols is an essential manual for newcomers in this ever-expanding and exciting area of research, as well as a valuable addition to more specialized laboratories.