1. An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility Navin Kumar Verma and Dermot Kelleher
2. Isolation of Human Peripheral Blood T Lymphocytes Atish Kizhakeyil, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, and Navin Kumar Verma
3. A Laboratory Model to Study T-Cell Motility Navin Kumar Verma, Sjyam Mohan Palapetta, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, Atish Kizhakeyil, and Dermot Kelleher
4. Quantification of T-Cell Migratory Phenotypes using High Content Analysis Aik Seng Ng, Seow Theng Ong, Dermot Kelleher, and Navin Kumar Verma
5. Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time Seow Theng Ong and Navin Kumar Verma
6. Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signalling Cross-Talks in Motile T-Cells Seow Theng Ong, Graham D. Wright, and Navin Kumar Verma
7. Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis Praseetha Prasannan and Navin Kumar Verma
8. Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-Lymphocytes Atish Kizhakeyil and Navin Kumar Verma
10. Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices using Confocal Microscopy Emmanuel Donnadieu, Yvonne Michel, and Martin-Leo Hansmann
11. Phosphoprotein Enrichment for Protein Analysis in Motile T-Lymphocytes Praseetha Prasannan, Rathi Saravanan, and Navin Kumar Verma
12. Immunometabolomic Phenotyping of Motile T-Cells Madhavi Latha Somaraju Chalasani, Gaelin Looi Wen Dong, and Navin Kumar Verma
13. Profiling Activity of Cellular Kinases in Migrating T-Cells Chandra Sekhar Chirumamilla, Mobashar Hussain Urf Turabe Fazil, Claudina Perez-Novo, Savithri Rangarajan, Rik de Wijn, Padma Ramireddy, Navin Kumar Verma, and Wim Vanden Berghe
14. Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes Malin Samuelsson and Lena M. Svensson
15. A Protocol to Study T-Cell Signalling in an Immune Synapse by Microscopy Mobashar Hussain Urf Turabe Fazil, Pankal Kumar, and Navin Kumar Verma
16. Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic Peptides Madhavi Latha Somaraju Chalasani, Rajamani Lakshminarayanan, and Navin Kumar Verma
17. Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility Geoffrey W. Payne, Kevin Mitchell, and Stephanie L. Sellers
18. Computational Analysis of Protein-Protein Interactions in Motile T-Cells Sunil Kumar, Mobashar Hussain Urf Turabe Fazil, Khursheed Ahmad, Minaketan Tripathy, Jagath Chandana Rajapakse, and Navin Kumar Verma
This volume discusses the latest developments in cellular, molecular, biochemical, and imaging assays to study the biology and functions of T-cells. The chapters in this book cover topics such as LFA-1/ICAM-1 interactions in T-cell motility; using 3D-SIM to dissect signaling cross-talks in motile T-cells; GapmeR-mediated gene silencing in motile T-cells; activity of cellular kinases in migrating T-cells; and computational analysis of protein-protein interactions in motile T-cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, T-Cell Motility: Methods and Protocols is an essential resource for graduate students, postdoctoral fellows, and principal investigators working in the fields of immunology, T-cell biology, biochemistry, molecular biology, and imaging.