1. Systems biology, synthetic biology and metabolic engineering2. Synthetic regulatory elements for fine-tuning gene expression3. Systems and synthetic biology-aided biosynthesis pathway design4. Refactoring and optimization of metabolic network5. Harnessing the hierarchy of transcriptional regulation: engineering of the gene expression network for efficient production of biofuels6. Systems metabolic engineering strategies for cell factories construction7. High throughput screening for improving cellular and enzymatic properties8. In vitro metabolic engineering: current status and recent progress9. Systems and synthetic metabolic engineering for production of biochemicals10. Systems and synthetic metabolic engineering: Challenges and prospects