Systemic Lupus Erythematosus: Methods and Protocols

ISBN-13: 9781493952625 / Angielski / Miękka / 2016 / 263 str.

Paul Eggleton; Frank J. Ward

Opis

Systemic Lupus Erythematosus: Methods and Protocols describe a number of genetic, biochemical and immunological techniques. These techniques provide an advancing understanding of the pathology, breakdown of the immune system and therapeutic challenges of SLE in both humans and animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Systemic Lupus Erythematosus: Methods and Protocols appeal to biomedical and clinical scientists in a number of pathology disciplines at the doctoral and post-doctoral level.

Spis treści

1. Pathology of Systemic Lupus Erythematosus - The Challenges Ahead

Pedro Correia Azevedo, Grainne Murphy, David Isenberg

2. The Lupus Biomarker Odyssey: One Experience

Joseph M. Ahearn, Susan Manzi, and Chau-Ching Liu 

3. Detection of Anti Nuclear Antibodies in SLE

Yashwant Kumar

4. Tests for Circulating Immune Complexes

Mark H. Wener

5. Methods for Measuring Antibody Dependent Cell-Mediated Cytotoxicity in vitro

Aaron S. Miller, Max L. Tejada, and Hélène Gazzano-Santoro

6. Recombinant Antibody Microarray for Profiling the Serum Proteome of SLE

Carl A.K. Borrebaeck, Gunnar Sturfelt, and Christer Wingren 

7. Bi-functional Antibody Fragment-based Fusion Proteins for the Targeted Elimination of Pathogenic T-cell Subsets

Wijnand Helfrich and Edwin Bremer

8. TLC Immunostaining for Detection of “Anti-Phospholipid” Antibodies

Fabrizio Conti, Cristiano Alessandri, Francesca Romana Spinelli, Antonella Capozzi, Francesco Martinelli, Serena Recalchi, Roberta Misasi, Guido Valesini, Maurizio Sorice

9. Induced Murine Models of Systemic Lupus Erythematosus

Yuan Xu, Leilani Zeumer, Westley H. Reeves, and Laurence Morel

10. Measuring Interferon Alpha and other Cytokines in SLE

Olferiev, Mikhail; Lliguicota, Mari; Kirou, Kyriakos A; Crow, Mary K.

11. Detection of SLE antigens in Neutrophils Extracellular Traps (NETs)

Carmelo Carmona-Rivera and Mariana J. Kaplan

12. Detection and Characterization of Autoantibodies Against Modified Self-proteins in SLE Sera after Exposure to Reactive Oxygen and Nitrogen Species

Brent J. Ryan and Paul Eggleton

13. Generation of Self-peptides to Treat Systemic Lupus Erythematosus

Jean-Paul Briand, Nicolas Schall and Sylviane Muller

14. Measurement of Malondialdehyde, Glutathione, and Glutathione Peroxidase in SLE Patients

Tamer A. Gheita and Sanaa A. Kenawy 

15. Evaluating a Particular Circulating MicroRNA Species from an SLE Patient using Stem Loop qRT-PCR

Weiguo Sui, Fuhua Liu, Jiejing Chen, Minglin ou, Yong Dai

16. Microarray Technology for Analysis of MicroRNA Expression in Renal Biopsies of Lupus Nephritis Patients

Weiguo Sui, Fuhua Liu, Jiejing Chen, Minglin ou, Yong Dai

17. Laboratory Tests for the Antiphospholipid Syndrome

Charis Pericleous, Vera M.Ripoll, Ian Giles, Yiannis Ioannou

18. Isolation, Polarisation, and Expansion of CD4⁺ T Helper Cell Lines and Clones using Magnetic Beads

Lekh N. Dahal, Robert N. Barker^, and Frank J. Ward

19. Meta-analysis as a Diagnostic Tool for Predicting Disease Onset and/or Activity in Systemic Lupus Erythematosus

Isabel Cottrell, Asma Khan, Sidra Maqsood, Jemma Thornes and Paul Eggleton. 

Blurb

Systemic Lupus Erythematosus: Methods and Protocols describes a number of genetic, biochemical and immunological techniques. These techniques provide an advancing understanding of the pathology, breakdown of the immune system, and therapeutic challenges of SLE in both humans and animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Systemic Lupus Erythematosus: Methods and Protocols  appeals to biomedical and clinical scientists in a number of pathology disciplines at the doctoral and post-doctoral level.