Synthetic Biology

ISBN-13: 9781627036245 / Angielski / Twarda / 2013 / 226 str.

Karen M. Polizzi; Cleo Kontoravdi

Opis

In Synthetic Biology, expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology. Focusing on computational tools that will aid in systematising the design and construction of parts and systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Synthetic Biology seeks to aid scientists in the further study of developing new biological components and systems.

Spis treści

Part I: Introduction

1. What is Synthetic Biology?

Karen M Polizzi

 

Part II: DNA Assembly

2. Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks Program

Gang Li, Bing-Xue Dong, Yu-Huan Liu, Chang-Jie Li, and Li-Ping Zhang

 

3. BioBrick^™ Assembly Using the In-Fusion PCR Cloning Kit

Sean C. Sleight and Herbert M. Sauro

 

4. Overlap Extension PCR Cloning

Anton Bryksin and Ichiro Matsumura

 

5. One-Step Isothermal Assembly of DNA Fragments

Rui TL Rodrigues and Travis S Bayer

 

 

PartIII: Part, Pathway, and Genome Engineering

6. Creation and Characterization of Component Libraries for Synthetic Biology

Tim Weenink and Tom Ellis

 

7. In vivo and in vitro Characterisation of s⁷⁰ Constitutive Promoter BioParts by Real-time PCR and Fluorescent Measurements

James Chappell and Paul Freemont

 

8. In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coli

Vandana Sharma and Yohei Yokobayashi

 

9. Construction and Engineering of  Large Biochemical Pathways via DNA Assembler

Zengyi Shao and Huimin Zhao

10. Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries through ePathBrick Vectors

Peng Xu and Mattheos A.G. Koffas

 

11. Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research

Matthias Haffke, Cristina Viola, Yan Nie, and Imre Berger

 

12. Combinatorial DNA Assembly using Golden Gate Cloning

Carola Engler and Sylvestre Marillonnet

 

13. Construction of Synthetic Gene Circuits in the Escherichia coli Genome

Bei-Wen Ying, Yuya Akeno, and Tetsuya Yomo

 14. Shuffling of DNA Cassettes in a Synthetic Integrin

David Bikard and Didier Mazel

 

 

Part IV: Computational Tools for Modelling Biological Systems

15. Systematic Methodology for the Development of Mathematical Models for Biological Processes

Cleo Kontoravdi

 

16. SOBOLHDMR: A General Purpose Modelling Software

Dr Sergei Kucherenko