1. Introduction to Synaptosome Methods and Applications
Kathryn M. Murphy
Part II Introduction to Synaptosomes
2. Victor P. Whittaker: The Discovery of the Synaptosome and Its Implications
Herbert Zimmermann
3. Synaptosome Preparations: Which Procedure Should I Use?
Peter R. Dunkley and Phillip J. Robinson
Part III Making Synaptosomes
4. Protocol for a High-Throughput Semi-Automated Preparation for Filtered Synaptoneurosomes
Justin L. Balsor and Kathryn M. Murphy
5. Subcellular Fractionation of Brain Tissue from Small Tissue Explants
Véronique De-Smedt-Peyrusse, Laetitia Darriet, Pierre Trifilieff, Etienne Herzog, and Maria Florencia Angelo
6. Preparation of P2 of Percoll-Purified Synaptosomes from Mammalian Brain Tissue
Jing Xue, Annie Quan, and Phillip J. Robinson
Part IV Studying Synaptic Energy and Metabolism using Synaptosomes
7. Synaptosomal Bioenergetics and Glutamate Release
David G. Nicholls
8. Synaptosomes Bioenergetics and Calcium Handling: Aging Response
Silvia Lores-Arnaiz, Georgina Rodriguez de Lores Arnaiz, Analia G. Karadayian, and Juanita Bustamante
9. Isolation of Highly Purified and Functionally Active Synaptic Mitochondria
Sergei V. Baranov, Nicolas K. Khattar, Svitlana Yablonska, Oxana V. Baranova, Eric S. Kretz, Timothy M. Larkin, Diane L. Carlisle, and Robert M. Freidlander
10. Affinity Proteomics for Interactome and Phosphoproteome Screening in Synaptosomes
Kasper Engholm-Keller, Nicolai Bache, Sushma R. Rao, Jesse R. Wark, Martin R. Larsen, Phillip J. Robinson, and Mark E. Graham
Part V Studying Synaptic Proteomics, Function, and Plasticity using Synaptosomes
11. Manipulating Synapse-Enriched Biochemical Fractions to Detect a Purified Synaptic Protein’s Effect on Neurotransmitter Release
Marianne Cilluffo, Theodore A. Sarafian, and Joseph B. Watson
12. Prosomes: Supramolecular Structures at the Synaptosome Membrane Involved in Vesicle Docking, Fusion, and Neurotransmitter Release
Bhanu P. Jena
13. SMPL-Synaptic Membranes: Nanodisc-Mediated Synaptic Membrane Mimetics Expand the Toolkit for Drug Discovery and the Molecular Cell Biology of Synapses
Riana Lo Bu, Jeffrey Clark, Thomas DiChiara, Stephen G. Sligar, and William L. Klein
Part VI Studying Development and Disease using Synaptosomes
14. Profiling of MicroRNA and Protein from Purified Synaptoneurosomes in a Neurodegenerative Disease Model
Amrit S. Boese, Anna Majer, and Stephanie A. Booth
15. Use of Synaptoneurosome Samples to Study Development and Plasticity of Human Cortex
Caitlin R. Siu, Simon P. Beshara, Justin L. Balsor, Steve J. Mancini, and Kathryn M. Murphy
16. The Use of Synaptosomes in Studying Autism Spectrum Disorder and Other Neurodevelopmental Disorders
Nadeem Murtaza, Vickie Kwan, Biren Dave, and Karun K. Singh
17. Flow Cytometric Evaluation of Crude Synaptosome Preparation as a Way to Study Synaptic Alteration in Neurodegenerative Diseases
Nadia P. Postupna, Caitlin S. Latimer, C. Dirk Keene, Kathleen S. Montine, Thomas J. Montine, and Martin Darvas
This volume describes techniques to make synaptosomes, and discusses numerous ways that synaptosomes are used to study and answer diverse questions about synapses. After examining the history that led to making synaptosomes, the chapters delved into its important as a tool to explore synaptic connections. Some of the topics covered in this book are: synaptosome bioenergetics and glutamate release; affinity proteomics for interactome and phosphoproteome screening in synaptosomes; isolation of highly purified and functionally active synaptic mitochondria; and profiling of microRNA and protein from purified synaptoneurosomes in a neurodegenerative disease model. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.
Cutting-edge and thorough, Synaptosomes is a valuable resource for any researcher interested in learning more about this developing field.
