Study of early spectral changes in cell transformation

ISBN-13: 9783639144369 / Angielski / Miękka / 2009 / 124 str.

Evgeny Bogomolny

Opis

Fourier Transform Infra-Red Microspectroscopy and Light Induced Fluorescence are two potentially powerful analytical methods for identifying the spectral properties of biological activity in cells. The goal of the present research is the implementation of both spectroscopic methods in tandem in order to study early spectral changes accompanying cancerous transformation. As a model for this purpose we used two kinds of cell cultures: Murine Fibroblast cell line and Murine Embryonic Fibroblast cells. The cell cultures were infected with Murine Sarcoma Virus, which induced cancerous transformation. The spectral measurements were taken at various post infection time intervals. Our results showed FTIR-MSP and LIF spectroscopic methods as promising optical tools for diagnosis of early stages of malignancy which is essential requirement for successful cancer treatment that cannot be accomplished by conventional methods (such as CT or MRI). Moreover, these methods are complimentary and shed light on the basic cellular metabolic changes occurring during the transformation, giving a better understanding of the carcinogenesis process.

O autorze

Bogomolny Evgeny;

Department of Physics,
Ben-Gurion University
Beer-Sheva, Israel;

e-mail: bogomole@bgu.ac.il;
2006- Ph.D. in Physics, Ben-Gurion University;
2002 - 2005: M.Sc. in Physics, Ben-Gurion University;
1998 - 2002: B.A. in Natural Science, Open University.