Strigolactones: Methods and Protocols

ISBN-13: 9781071614280 / Angielski / Twarda / 2021 / 262 str.

Cristina Prandi; Francesca Cardinale

Spis treści

 

Part I STRIGOLACTONE CHEMISTRY

1. Evaluation and Quantification of Natural Strigolactones from Root Exudates

Xiaonan Xie, Kaori Yoneyama, and Takahito Nomura Koichi Yoneyama

 

2. Isolation and Identification of Naturally Occurring Strigolactones

Kotomi Ueno, Takatoshi Wakabayashi, and Yukihiro Sugimoto

 

3. Chemical Synthesis of Triazole-Derived Suppressors of Strigolactone Functions

 Shisanku Ito, Ko Kikuzato, Hidemitsu Nakamura, and Tadao Asami

 

4. Synthesis of Simple Strigolactone Mimics

Tomáš Pospíšil

 

5. Synthesis of Strigolctones Analogues and Evaluation of their Stability in Solution

Daniel Blanco-Ania and Binne Zwanenburg

 

Part II: STRIGOLACTONES and SOIL BIOTA

6. Strigolactone-like Bioactivity Via Parasitic Plant Germination Bioassay

Jean-Bernard Pouvreau, Lucie Poulin, Sarah Huet, and Philippe Delavault

 

7. Evaluation of the Effect of Strigolactones and Synthetic Analogues On Fungi

Valentina Fiorilli, Mara Novero, and Luisa Lanfranco

 

8. Analysing the Effect of Strigolactones on the Motility Behaviour of Rhizobia

Lydia M. Bernabéu-Roda, Juan Antonio López-Ráez, and María J. Soto

 

9. Chemotropic Assay for Testing Fungal Response to Strigolactones and Strigolactone-Like Compounds

Rocío Pineda-Martos, Antonio Di Pietro, and Davide Turrà

 

Part III: STRIGOLACTONES AS PLANT HORMONES

10. Methods for Phenotyping Shoot Branching and Testing Strigolactone Bioactivity for Shoot Branching in Arabidopsis and Pea

Aitor Muñoz, Jean-Paul Pillot, Pilar Cubas, and Catherine Rameau

 

11. Bioassays for the Effects of Strigolactones and Other Small Molecules on Root and Root Hair Development

José Antonio Villaécija-Aguilar, Sylwia Struk, Sofie Goormachtig, and Caroline Gutjahr

 

12. Methods for Medium-Scale Study of the Biological Effects of Strigolactone-Like Molecules on the Moss Physcomitrella patens

Ambre Guillory and Sandrine Bonhomme

 

13. Controlled Assays for Phenotyping the Effects of Strigolactone-like Molecules on Arbuscular Mycorrhiza Development

Salar Torabi, Kartikye Varshney, José A. Villaécija-Aguilar, Andreas Keymer, and Caroline Gutjahr

 

14. Application of Strigolactones to Plant Roots to Influence the Formation of Symbioses

Eloise Foo

 

Part IV: BIOACTIVITY ASSAYS

15.Evaluation of the Bioactivity Oof Strigolactone-Related Molecules by a Quantitative Luminometer Bioassay

Elena Sánchez, Pilar Cubas, Francesca Cardinale, and Ivan Visentin

 

16. A Protoplast-based Bioassay to Quantify Strigolactone Activity in Arabidopsis using StrigoQuant

Justine Braguy, Sophia L. Samodelov, Jennifer Andres, Rocio Ochoa-Fernandez, Salim Al Babili, and  Matias D. Zurbriggen

 

17. Synthesis of Profluorescent Strigolactone Probes for Biochemical Studies

Alexandre de Saint Germain, Guillaume Clavé, and François-Didier Boyer

 

18. The use of Differential Scanning Fluorimetry to Assess Strigolactone Receptor Function

Cyril Hamiaux, Bart J Janssen, and Kimberley Snowden

 

19. Structural Analysis of Strigolactone-Related Gene Products

Gunilla H. Carlsson, Dirk Hasse, and Inger Andersson

Blurb

This volume presents the most useful laboratory protocols in strigolactones(SL) research. Chapters guide readers through wet-lab paths, issues around stability, protocols to evaluate SL activity, effects towards soil inhabitants such as parasitic plants, mycorrhizal and non-mycorrhizal fungi, nodulating bacteria, and protocols to assess effects on plant development are discussed. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Strigolactones: Methods and Protocols aims to deliver a clear-cut and standardized set of experimental protocols to a broad scientific community.