Streptococcus Pneumoniae: Methods and Protocols

ISBN-13: 9781493991983 / Angielski / Twarda / 2019 / 227 str.

Federico Iovino

Recenzje

"The audience includes students, researchers, and microbiologists, as well as others in the fields of public health, epidemiology, and biostatistics related to Streptococcus pneumoniae. ... This is an excellent book on Streptococcus pneumoniae, with additional coverage of the related public health, epidemiology, and biostatistics aspects. I am sure that the information in this book will be of much help and interest to the intended audience." (Omer Iqbal, Doody's Book Reviews, December 7, 2019)

Spis treści

Editor Biography…

Preface…

Table of Contents…

Contributing Authors…

Part I Cultivation of Streptococcus pneumoniae In Vitro

1. Optimal Conditions for Streptococcus pneumoniae Culture: In Solid and Liquid Media

Esther Texeira and Norma Suárez

Part II Microscopy Techniques to Study the Biology of Streptococcus pneumoniae and Pneumococcal Interactions with the Host

2. Electron Microscopy to Study the Fine Structure of the Pneumococcal Cell

Sven Hammerschmidt and Manfred Rohde

3. Immunofluorescent Staining and High-Resolution Microscopy to Study the Pneumococcal Cell

Federico Iovino and Birgitta Henriques-Normark

4. Construction of Fluorescent Pneumococci for In Vivo Imaging and Labeling of the Chromosome

Morten Kjos

5. High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host

Federico Iovino and Birgitta Henriques-Normark

Part III The Genetics of Streptococcus pneumoniae

6. Natural Genetic Transformation: A Direct Route to Easy Insertion of Chimeric Genes into the Pneumococcal Chromosome

Isabelle Mortier-Barriere, Nathalie Campo, Mathieu Berge, Marc Prudhomme, and Patrice Polard

7. Gene Expression Analysis in the Pneumococcus

Rory A. Eutsey, Carol A. Woolford, Surya D. Aggarwal, Rolando A. Cuevas, Luisa N. Hiller 

8. Transcriptional Knockdown in Pneumococci using CRISPR Interference

Morten Kjos

Part IV The Proteome and Proteomics of Streptococcus pneumoniae 

9. Protein Expression Analysis by Western Blot and Protein-Protein Interactions

María Dolores Cima-Cabal, Fernando Vázquez, Juan R. de los Toyos, and María del Mar García-Suárez 

10. Mass Spectrometry to Study the Bacterial Proteome from a Single Colony

Jianwei Zhou, Lu Zhang, Huixia Chuan, Angela Sloan, Raymond Tsang, Keding Cheng 

11. Bead-Based Flow Cytometric Cell Counting of Live and Dead Bacteria

Fang Ou, Cushla McGoverin, Joni White, Simon Swift, and Frédérique Vanholsbeeck 

Part V Streptococcus pneumoniae-Host Interactions: In Vitro and In Vivo Models

12. In Vitro Adhesion, Invasion, and Transcytosis of Streptococcus pneumonia with Host Cells

Terry Brissac and Carlos J. Orihuela

13. Growing and Characterizing Biofilms Formed by Streptococcus pneumoniae

Yashuan Chao, Caroline Bergenfelz, and Anders P. Hakansson

14. In Vivo Mouse Models to Study How Pneumococci Interact with the Host and Cause Invasive Pneumococcal Disease

Vicky Sender, Birgitta Henriques-Normark, and Federico Iovino

15. Two-Photon Intravital Imaging of Leukocytes in the Trachea during Pneumococcal Infection

Miguel Palomino-Segura and Santiago F. Gonzalez

16. IVIS Spectrum CT to Image the Progression of Pneumococcal Infections In Vivo

Adam Sierakowiak, Birgitta Henriques-Normark, and Federico Iovino

Part VI Public Health, Epidemiology, and Biostatistics

17. The Pneumococcus and Its Critical Role in Public Health

Godwin Oligbu, Norman K. Fry, and Shamez Ladhani

18. The Epidemiology and Biostatistics of Pneumococcus

Godwin Oligbu, Norman K. Fry, and Shamez Ladhani

Blurb

This volume looks at the newest methods, materials, equipment, and technologies developed to study the cell biology of the pneumococcus. The chapters, which cover a wide range of topics, are organized into six parts: Part one discusses the cultivation of Streptococcus pneumoniae in vitro; Part Two talks about the microscopy techniques used to study the biology of Streptococcus pneumoniae and pneumococcal interactions with the host; Parts Three and Four describe the genetics, proteome, and proteomics of Streptococcus pneumoniae; Part Five explains in vitro and in vivo models of Streptococcus pneumoniae-host interactions; and Part Six looks at the factors of public health, epidemiology, and biostatistics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Cutting-edge and authoritative, Streptococcus pneumoniae: Methods and Protocols is a valuable resource for both novice and expert researchers who want to expand their studies into new areas and new systems in this continuously evolving field.