Stem cells : In vitro neurogenesis of human and rat bone marrow derived mesenchymal stem cells

ISBN-13: 9783659858338 / Angielski / Miękka / 2016 / 144 str.

Sarah Al-Sawalha; Jabbar Yanzeel; Abdul Majeed Hammadi

Opis

This study aimed to investigate the ability of mesenchymal stem cells derived from bone marrow of human and rat to transdifferentiate into functional neural like cells. A study was made on two different models (human and rat) using two different techniques (direct plating, and density gradient method) respectively. Immunophenotypic analysis demonstrated that human and rat BM-MSCs at passage three uniformly positive for CD44 sub-population. However, MSCs were always found to be negative for heamatopoietic specific markers CD34. To explore the possibility of BM-MSCs in vitro transdifferentiation, the expanded MSCs from third passage were allowed to reach 80% confluence and induced to differentiate into neuron like cells by an enhanced three-step protocol. The identity of the NLCs were illustrated by the analysis of morphology using immunocytochemistry.

O autorze

Sarah M. S. Al-Sawahha EDUCATION:April. 2015 - Baghdad University, Candidate for master degree in Biology\ animal physiology.Jun 2012, - Baghdad University,Candidate for Bachelor in Biology.EMPLOYMENT HISTORY:Sep.2015Lab technician in stem cell culture in Alzahrawy center for stem cells therapy.