Stem Cells for Myocardial Regeneration: Methods and Protocols

ISBN-13: 9781493961269 / Angielski / Miękka / 2016 / 267 str.

Randall J. Lee

Spis treści

Stem Cells for Myocardial Regeneration: Methods and Protocols, Table of Contents: Table of Contents Preface Contributors Introduction: 1. Stem Cells for Myocardial Repair and Regeneration – Where are We Today? – Randall J. Lee Section 1: Isolation and Characterization of Cells for Myocardial Regeneration 2. Bone Marrow-derived Endothelial Progenitor Cells: Isolation and Characterization for Myocardial Repair - Masaaki Ii 3. Umbilical Stem Cells for Myocardial repair and regeneration – Nick Greco and Mary Laughlin 4. Isolation of Resident Cardiac Progenitor Cells by Hoechst 33342 Staining– Otmar Pfister, Angelos Oikonomopoulos, Konstantina-Ioanna Sereti, and Ronglih Liao 5. Mesenchymal Stem Cell Therapy For Cardiac Repair – Andrew J Boyle, Ian K. McNiece, and Joshua M. Hare 6. Methods for Human Embryonic Stem Cells derived Cardiomyocytes cultivation, genetic manipulation and transplantation – Gil Arbel, Oren Caspi, Irit Huber, Amira Gepstein, Michal Weiler-Sagie, Lior Gepstein Section 2: Animal Models for Assessing Stem Cells for Myocardial Regeneration 7. Experimental Cell Transplantation Therapy In Rat Myocardial Infarction Model Including Nude Rat Preparation – Wangde Dai and Robert A. Kloner 8. Large Animal Model of Heart Failure for Assessment of Stem Cells– Sharad Rastogi Section 3: Histological Assessment of Myocardial Regeneration 9. Histopathologic Assessment of Myocardial Regeneration - Naima Carter-Monroe, Elena Ladich, Renu Virmani, and Frank D. Kolodgie 10. Assessment of Myocardial Angiogenesis and Vascularity in Small Animal Models – Matt Springer Section 4: Measures of Cell trafficking 11. Superparamagnetic Iron Oxide Labeling of Stem Cells for MRI Tracking and Delivery in Cardiovascular Disease - Dorota A. Kedziorek and Dara L. Kraitchman 12. Embryonic Stem Cell Biology: Insights from Molecular Imaging – Karim Sallam, Joseph C. Wu 13. Genetic Fate-Mapping for Studying Adult Cardiomyocyte Replenishmentafter Myocardial Injury - Sunny S.-K. Chan, Ying-Zhang Shueh, Nirma Bustamante, Hua-Lin Wu, Jyh-Hong Chen, Patrick C. H. Hsieh Section 5: Electrophysiology 14. In Vitro assessment of cell communication – Seth Weinberg, Elizabeth A. Lipke, and Leslie Tung 15. Assessment of Cardiac Conduction – Basic Principles of Optical Mapping– Chunhua Ding and Thomas H. Everett, IV Section 6: Biomaterials 16. Surface Patterning for Generating Defined Nano-Scale Matrices – Karen Christman and Heather Maynard

Blurb

There is currently a great deal of enthusiasm surrounding organ regeneration, which mainly comes from the potential pleuripotency of stem cells to differentiate into various tissue types. In Stem Cells for Myocardial Regeneration: Methods and Protocols, expert researchers explore exciting new developments in the field of regenerative medicine, focusing both on the benefits of using stem cells in myocardial repair and regeneration and on the challenges associated with making this a mainstream therapy. Chapters consider the optimal cell type for myocardial repair, examine the developmental processes of the human heart in order to develop strategies for regeneration, consider ex-vivo optical mapping and in vivo electrophysiology studies, and investigate the effects of the extracellular matrix on stem cell renewal and differentiation. Composed in the highly successful Methods in Molecular Biology(tm) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Ground-breaking and current, Stem Cells for Myocardial Regeneration: Methods and Protocols, is an essential guide for scientists, translational investigators, and cardiologists alike, providing readers with a means to evaluate the efficacy and safety of adopting stem cells as the standard for myocardial regeneration.