Stem Cells and Tissue Repair: Methods and Protocols

ISBN-13: 9781071606575 / Angielski / Miękka / 2021 / 214 str.

Chrissa Kioussi

Spis treści

Preface…
Table of Contents…
Contributing Authors…

1. Culturing and Manipulating Mouse Embryonic Stem Cells
Chrissa Kioussi

2. Generation of an Induced Pluripotent Stem Cell Line with the Constitutive EGFP Reporter 
Kiel T. Carson Butterfiel, Patrick S. McGrath, Chann Makara Han, Igor Kogut, and Ganna Bilousova

3. Engraftable Induced Pluripotent Stem Cell-Derived Neural Precursors for Brain Repair
Ourania Zygogianni, Georgia Kouroupi, Era Taoufik, and Rebecca Matsas

4. In Vitro Direct Reprogramming of Mouse and Human Astrocytes to Induced Neurons
Katerina Aravantinou-Fatorou and Dimitra Thomaidou

5. Preparation of Bioscaffolds Delivering Stem Cells for Neural Regeneration 
Li Yao and Ashley DeBrot

6. Improved Isolation of Human Vascular Wall-Resident Mesenchymal Stem Cells
Diana Klein

7. In Vitro Generation of Vascular Wall-Typical Mesenchymal Stem Cells (VW-MSC) from Murine Induced Pluripotent Stem Cells through VW-MSC Specific Gene Transfer
Jennifer Steens, Hannes Klump, and Diana Klein

8. Analysis of Tooth Innervation in Microfluidic Co-Culture Devices
Pierfrancesco Pagella and Thimios A. Mitsiadis

9. Peripheral Nerve Regeneration in a Novel Rat Model of Dysphagia
Kiyoshi Sakai, Takeshi Tsuruta, Junna Watanabe, Yukiko Sugimura, Kohei Sakaguchi, Wataru Katagiri, and Hideharu Hibi

0. Healing of Full Thickness Murine Skin Wounds Containing Nanofibers using Splints for Efficient Re-Epithelialization and to Avoid Contracture
Nilika Bhattacharya, Arup K. Indra, and Gitali Ganguli-Indra

11. Enrichment and Characterization of Human and Murine Pulmonary Mesenchymal Progenitor Cells (MPC)
Megan Summers, Karen Helm, and Susan M. Majka

12. Isolation and Culture of Quiescent Skeletal Muscle Satellite Cells 
Francisco Hernández-Torres, Lara Rodríguez-Outeiriño, and Amelia Aránega

13. Isolation, Culture, Cryopreservation and Identification of Bovine, Murine, and Human Spermatogonial Stem Cells 
Pedro M. Aponte

14. Long-Term Ex Vivo Expansion of Murine Spermatogonial Stem Cells in a Simple Serum-Free Medium
Hiroshi Kubota and Kazue Kakiuchi

15. Generating Kidney Organoids from Human Pluripotent Stem Cells Using Defined Conditions
Sara E. Howden and Melissa H. Little

16. Painting the Pancreas in Three Dimensions: Whole Mount Immunofluorescence Method
Maricela Maldonado, Jeffrey D. Serrill, and Hung-Ping Shih

17. Induction of Osteoblasts by Direct Reprogramming of Mouse Fibroblasts 
Hui Zhu and Joy Wu

Blurb

This volume looks at a collection of stem cell and regenerative techniques used by both novice and expert scientists. Chapters cover topics such as tissue repaired by expansion and reprogramming; induced pluripotent stem cells driven in neuronal or vascular differentiation; using mesenchymal stem cells to derive skeletal muscle, osteoblasts, and spermatogonial cells; and the technique of monitoring the development of sub-organ microenvironments in the developing pancreas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Stem Cells and Tissue Repair: Methods and Protocols, Second Edition is a valuable resource that provides readers with the latest descriptions and references for exploring this vast field in regenerative medicine.