Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations

ISBN-13: 9781493924349 / Angielski / Twarda / 2015 / 220 str.

Kursad Turksen

Opis

This volume collects a series of protocols describing the kinds of infrastructures, training, and standard operating procedures currently available to actualize the potential of stem cells for regenerative therapies. Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations pulls together key GMP techniques from laboratories around the world. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Inclusive and authoritative, Stem Cells and Good Manufacturing Practices: Methods, Protocols, and Regulations will be an invaluable resource to both basic and clinical practitioners in stem cell biology.

Spis treści

1. Therapeutic Mesenchymal Stromal Cells: Where We Are Headed

            Patrick J. Hanley

 

2. The Suspension Culture of Undifferentiated Human Pluripotent Stem Cells Using Spinner Flasks

            Vincent C. Chen and Larry A. Couture

 

3. Scalable Expansion of Human Induced Pluripotent Stem Cells in Xeno-Free Microcarriers

            Sara M. Badenes, Tiago G. Fernandes, Carlos A.V. Rodrigues, Maria Margarida

Diogo, and Joaquim M.S. Cabral

 

4. Derivation of GMP-Compliant Integration-Free hiPSCs Using Modified mRNAs

            Jens Durruthy-Durruthy and Vittorio Sebastiano

 

5. Labeling Pluripotent Stem Cell-Derived Neural Progenitors with Iron Oxide Particles for Magnetic Resonance Imaging

            Sébastien Sart, Fabian Calixto Bejarano, Yuanwei Yan, Samuel C. Grant, and Yan Li

 

6. Using the Quantum Cell Expansion System for the Automated Expansion of Clinical-Grade Bone Marrow-Derived Human Mesenchymal Stromal Cells

            Gema Martin-Manso and Patrick J. Hanley

 

7. Isolation and Expansion of Mesenchymal Stromal/Stem Cells from Umbilical Cord Under Chemically Defined Conditions

            Heba Badraiq, Liani Devito, and Dusko Ilic

 

8. Production of Good Manufacturing Practice-Grade Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells for Therapeutic Use

            Phuc Van Pham and Phan Kim Ngoc

 

9. Xeno-Free Culture of Human Periodontal Ligament Stem Cells

            Oriana Trubiani and Francesca Diomede

 

10. GMP-Compliant Human Adipose Tissue-Derived Stromal Cells for Cellular Therapy

            Hamid-Reza Aghayan, Parisa Goodarzi, and Babak Arjmand

 

11. cGMP-Compliant Transportation Conditions for a Prompt Therapeutic Use of Marrow Mesenchymal Stromal/Stem Cells

            Elena Veronesi, Jorge Sans Burns, Alba Murgia, Olivia Candini, Valeria Rasini,

Ilenia Mastrolia, Fabio Catani, Paolo Paolucci, and Massimo Dominici

 

12. GMP-Grade Human Fetal Liver-Derived Mesenchymal Stem Cells for Clinical Transplantation

            Bagher Larijani, Hamid-Reza Aghayan, Parisa Goodarzi, and Babak Arjmand

 

13. Purification of Human Induced Pluripotent Stem Cell-Derived Neural Precursors Using Magnetic Activated Cell Sorting

            Gonçalo M.C. Rodrigues, Tiago G. Fernandes, Carlos A.V. Rodrigues, Joaquim M.S. Cabral, and Maria Margarida Diogo

 

14. Scalable Ex Vivo Expansion of Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Stirred Culture Systems

            Joana G. Carmelo, Ana Fernandes-Platzgummer, Joaquim M.S. Cabral, and Cláudia Lobato da Silva

 

15. Media Fill for Validation of a Good Manufacturing Practice-Compliant Cell Production Process

            Marta Serra, Livia Roseti, and Alessandra Bassi

 

16. Standard Operating Procedure for the Good Manufacturing Practice-Compliant Production of Human Bone Marrow Mesenchymal Stem Cells

            Livia Roseti, Marta Serra, and Alessandra Bassi

 

17. Culture of Human Limbal Epithelial Stem Cells on Tenon’s Fibroblast Feeder-Layers: A Translational Approach

            Gaia Scafetta, Camilla Siciliano, Giacomo Frati, and Elena De Falco

 

18. Bioreactor Expansion of Human Mesenchymal Stem Cells According to GMP Requirements

            Christiane L. Elseberg, Denise Salzig, and Peter Czermak