Stem Cells and Aging: Methods and Protocols

ISBN-13: 9781627033169 / Angielski / Twarda / 2013 / 179 str.

Kursad Turksen

Opis

Over time, it has become clear that changes in stem cells do occur during aging, not only in their number but also in their relationship to their microenvironment and their functionality as reflected in changes to their metabolome. Stem Cells and Aging: Methods and Protocols brings together chapters from expert contributors with protocols critical for exploring the biology of stem cell aging, all of which is key for understanding these age-related stem cell changes at a basic biology level and at the level of their impacts for regenerative medicine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Concise and easy to use, Stem Cells and Aging: Methods and Protocols serves as an ideal reference to guide investigators toward further valuable answers to the problems of our aging population.

Spis treści

1. Comprehensive Hematopoietic Stem Cell Isolation Methods

            Kyle Rector, Yi Liu, and Gary Van Zant

 

2. Serial Transplantation of Bone Marrow to Test Self-Renewal Capacity of Hematopoietic Stem Cells In Vivo

            Charusheila Ramkumar, Rachel M. Gerstein, and Hong Zhang

 

3. Mouse Hematopoietic Stem Cell Transplantation

            Hui Cheng, Paulina H. Liang, and Tao Cheng

 

4. Isolation, Characterization, and Transplantation of Adult Liver Progenitor Cells

            Mladen I. Yovchev, Mariana D. Dabeva, and Michael Oertel

 

5. Isolation of Muscle-Derived Stem/Progenitor Cells Based on Adhesion Characteristics to Collagen-Coated Surfaces

            Mitra Lavasani, Aiping Lu, Seth D. Thompson, Paul D. Robbins, Johnny Huard, and Laura J. Niedernhofer

 

6. Human Myoblasts from Skeletal Muscle Biopsies: In Vitro Culture Preparations for Morphological and Cytochemical Analyses at Light and Electron Microscopy

            Manuela Malatesta, Marzia Giagnacovo, Rosanna Cardani, Giovanni Meola, and Carlo Pellicciari

 

7. Cardiac Stem Cell Senescence

            Daniela Cesselli, Federica D’Aurizio, Patrizia Marcon, Natascha Bergamin, Carlo Alberto Beltrami, and Antonio Paolo Beltrami

 

8. Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxygen Conditions

            Sebastian Klepsch, Angelika Jamnig, Daniela Trimmel, Magdalena Schimke, Werner Kapferer, Regina Brunauer, Sarvpreet Singh, Stephan Reitinger, and Günter Lepperdinger

 

9. Methods for Assessing Effects of Wnt/β-Catenin Signaling in Senescence of Mesenchymal Stem Cells

            Hai-jie Wang and Yu-zhen Tan

 

10. Intra-Femoral Injection of Human Mesenchymal Stem Cells

            Sindhu T Mohanty and Ilaria Bellantuono

 

11. Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency

            Anne Schellenberg, Hatim Hemeda, and Wolfgang Wagner

 

12. Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenetic Regulation of Stem Cell Aging

            Seunghee Lee, Ji-Won Jung, and Kyung-Sun Kang

 

13. Quantitative Fluorescence In Situ Hybridization (Q-FISH) on Paraffine Embedded Tissue

            Mario Ricciardi, Mauro Krampera, and Marco Chilosi

            Daniela Cesselli, Federica D’Aurizio, Patrizia Marcon, Natascha Bergamin, Carlo Alberto Beltrami, and Antonio Paolo Beltrami

 

8. Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxygen Conditions

            Sebastian Klepsch, Angelika Jamnig, Daniela Trimmel, Magdalena Schimke, Werner Kapferer, Regina Brunauer, Sarvpreet Singh, Stephan Reitinger, and Günter Lepperdinger

 

9. Methods for Assessing Effects of Wnt/β-Catenin Signaling in Senescence of Mesenchymal Stem Cells

            Hai-jie Wang and Yu-zhen Tan

 

10. Intra-Femoral Injection of Human Mesenchymal Stem Cells

            Sindhu T Mohanty and Ilaria Bellantuono

 

11. Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency

            Anne Schellenberg, Hatim Hemeda, and Wolfgang Wagner

 

12. Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenetic Regulation of Stem Cell Aging

            Seunghee Lee, Ji-Won Jung, and Kyung-Sun Kang

 

13. Quantitative Fluorescence In Situ Hybridization (Q-FISH) on Paraffine Embedded Tissue

            Mario Ricciardi, Mauro Krampera, and Marco Chilosi

            Anne Schellenberg, Hatim Hemeda, and Wolfgang Wagner

 

12. Chromatin Immunoprecipitation Coupled by Quantitative Real-Time PCR as a Tool for Analyzing Epigenetic Regulation of Stem Cell Aging

            Seunghee Lee, Ji-Won Jung, and Kyung-Sun Kang

 

13. Quantitative Fluorescence In Situ Hybridization (Q-FISH) on Paraffine Embedded Tissue

            Mario Ricciardi, Mauro Krampera, and Marco Chilosi

            Seunghee Lee, Ji-Won Jung, and Kyung-Sun Kang

 

13. Quantitative Fluorescence In Situ Hybridization (Q-FISH) on Paraffine Embedded Tissue

            Mario Ricciardi, Mauro Krampera, and Marco Chilosi

            Mario Ricciardi, Mauro Krampera, and Marco Chilosi