Stem Cells and Aging: Methods and Protocols

ISBN-13: 9781493997121 / Angielski / Twarda / 2019 / 354 str.

Kursad Turksen

Spis treści

1. Assessing Muscle Stem Cell Clonal Complexity during Aging

            Matthew T. Tierney, Michael J. Stec, and Alessandra Sacco

 

2. Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies

            Eusebio Perdiguero, Victoria Moiseeva, and Pura Muñoz-Cánoves

 

3. Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle

            Sören S. Hüttner, Hellen E. Ahrens, Manuel Schmidt, Henriette Henze, Marie Juliane Jung, Svenja C. Schüler, and Julia von Maltzahn

 

4. Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence of Human Mesenchymal Stem Cells from Adipose Tissue

            Meenakshi Gaur, Marek Dobke, and Victoria V. Lunyak

 

5. Quantifying Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal Cell Cultures

            Stéphanie Nadeau, Anastasia Cheng, Inés Colmegna, and Francis Rodier

 

6. Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells

            Courtney R. Ogando, Gilda A. Barabino, and Yueh-Hsun Kevin Yang

 

7. Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cell Isolation and Growth Kinetics Analysis

            Klemen Čamernik, Janja Marc, and Janja Zupan

 

8. Complete Assessment of Multilineage Differentiation Potential of Human Skeletal-Muscle-Derived Mesenchymal Stem/Stromal Cells

            Klemen Čamernik and Janja Zupan

 

9. Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis

            Janja Zupan

 

10. 3D-Embedded Cell Cultures to Study Tendon Biology

            Renate Gehwolf, Gabriel Spitzer, Andrea Wagner, Christine Lehner, Nadja Weissenbacher, Herbert Tempfer, and Andreas Traweger

 

11. Targeted, Amplicon-Based, Next-Generation Sequencing to Detect Age-Related Clonal Hematopoiesis

            Brooke Snetsinger, Christina Ferrone, and Michael J. Rauh

 

12. Column Free Method for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants

            Sherin Saheera and Renuka R. Nair

 

13. Histological Assessment of Cre/loxP Genetic Recombination in the Aging Subventricular Zone of Nestin-CreER^T2/Rosa26YFP Mice

            Saad Omais, Nour N. Halaby, Karl John Habashy, Carine Jaafar, Anthony T. Bejjani, and Noël Ghanem

 

14. Infrared Spectroscopy and Imagıng in Stem Cells and Agıng Research

            Ceren Aksoy and  Feride Severcan

 

15. Use of U-STELA for Accurate Measurement of Extremely Short-Telomeres

            Nedime Serakinci, Huseyin Cagsin, and Merdiye Mavis

 

16. Surface-Antigen-Based Identification of In Vitro Expanded Skeletal-Muscle-Derived Mesenchymal Stromal/Stem Cells Using Flow Cytometry

            Klemen Čamernik and Janja Zupan

 

17. Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry

            Rasmus Jentoft Boutrup

 

18. Methods for Detection of Autophagy in Mammalian Cells

            Bindu Singh and Sangeeta Bhaskar

 

19. Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells

            Yohei Hayashi and Yasuhisa Matsui

 

20. Reprogramming of Aged Cells into Pluripotent Stem Cells by Nuclear Transfer

            Dan-Ya Wu, Xia Zhang, and Yi-Liang Miao

 

21. Generation of Retinal Pigmented Epithelial (RPE) Cells for Treatment of Age-Related Macular Degeneration (AMD)

            Harshini Surendran, Reena J. Rathod, and Rajarshi Pal

 

22. Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury

            Jea-Young Lee, Roger Lin, Hung Nguyen, M. Grant Liska, Trenton Lippert, Yuji Kaneko, and Cesar V. Borlongan

 

23. 3D Age-Specific Mortality Trajectory: A Survival Analysis Protocol

            Yuhui Lin

 

24. Isolation, Expansion, and Characterization of Wharton’s Jelly-Derived Mesenchymal Stromal Cell: Method to Identify Functional Passages for Experiments

            Shuh-Wen Aung, Noor Hayaty Abu Kasim, and Thamil Selvee Ramasamy

 

25. CRISPR Base Editing in Induced Pluripotent Stem Cells

            Ya-Ju Chang, Christine L. Xu, Xuan Cui, Alexander G. Bassuk, Vinit B. Mahajan, Yi-Ting Tsai, and Stephen H. Tsang

Blurb

This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy to follow fashion so as to be valuable for not only experts but novices in the ever-developing stem cell field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Stem Cells and Aging: Methods and Protocols, Second Edition serves as an ideal guide for all researchers working in this very active area of study.