Stem Cell Renewal and Cell-Cell Communication: Methods and Protocols

ISBN-13: 9781493925896 / Angielski / Twarda / 2015 / 210 str.

Kursad Turksen

Opis

This volume examines cell-cell interactions and stem cell renewal, two topics that are now inexorably linked as science strives to understand the stem cell niche and its function. Gathering a number of representative protocols, this detailed collection promises to provide readers with approaches for studying these complimentary aspects of stem cells. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Practical and reliable, Stem Cell Renewal and Cell-Cell Communication: Methods and Protocols will aid researchers in using these methods to advance their own studies.

Spis treści

1. Laboratory Control and Basic Culture Protocols for Stem Cell Self-Renewal

            Jeong Mook Lim, Yoon Mi Jo, and Ji Yeon Ahn

 

2. Advanced Fixation for Transmission Electron Microscopy Unveils Special Extracellular Matrix Within the Renal Stem/Progenitor Cell Niche

            Will W. Minuth and Lucia Denk

 

3. Multiplex Immunoassays for Quantification of Cytokines, Growth Factors, and Other Proteins in Stem Cell Communication

            Ivona Valekova, Helena Kupcova Skalnikova, Karla Jarkovska, Jan Motlik, and Hana Kovarova

 

4. Porous Membrane Culture Method for Expansion of Human Pluripotent Stem Cells

            Jin-Su Kim, Seung-Taeh Hwang, and Soo-Hong Lee

 

5. Tuning Differentiation Signals for Efficient Propagation and In Vitro Validation of Rat Embryonic Stem Cell Cultures

            Stephen Meek, Linda Sutherland, and Tom Burdon

 

6. Generation, Expansion, and Differentiation of Human Pluripotent Stem Cell (hPSC) Derived Neural Progenitor Cells (NPCs)

            David A. Brafman

 

7. Isolation, Long-Term Expansion, and Differentiation of Murine Neural Stem Cells

            Alexandra Bizy and Sacri R. Ferrón

 

8. Generation, Expansion, and Differentiation of Cardiovascular Progenitor Cells from Human Pluripotent Stem Cells

            Nan Cao, He Liang, and Huang-Tian Yang

 

9. A Practical Guide for the Isolation and Maintenance of Stem Cells from Tendon

            Pauline Po Yee Lui

 

10. A Human Colonic Crypt Culture System to Study Regulation of Stem Cell-Driven Tissue Renewal and Physiological Function

            Alyson Parris and  Mark R. Williams

 

11. Ca²⁺ Handling in Mouse Embryonic Stem Cell Derived Cardiomyocytes

            Wenjie Wei and Jianbo Yue

 

12. Potential Application of Extracellular Vesicles of Human Adipose Tissue-Derived Mesenchymal Stem Cells in Alzheimer’s Disease Therapeutics

            Takeshi Katsuda, Katsuyuki Oki, and Takahiro Ochiya

 

13. Application of Fluid Mechanical Force to Embryonic Sources of Hemogenic Endothelium and Hematopoietic Stem Cells

            Nan Li, Miguel F. Diaz, and Pamela L. Wenzel

 

14. Electrophysiological Recordings from Neuroepithelial Stem Cells

            Masayuki Yamashita

 

15. In Vivo Stem Cell Transplantation Using Reduced Cell Numbers

            Takeo W. Tsutsui