Stem Cell Niche: Methods and Protocols

ISBN-13: 9781493995103 / Angielski / Miękka / 2020 / 199 str.

Kursad Turksen

Spis treści

1. In Vitro Maintenance of Multipotent Neural Crest Stem Cells as Crestospheres

            Sofie Mohlin and Laura Kerosuo

 

2. Analysis of Hematopoietic Niche in the Mouse Embryo

            Keai Sinn Tan, Nathalie Brouard, and Daisuke Sugiyama

 

3. Functional Assays of Stem Cell Properties Derived from Different Niches

            Beatriz de Lucas, Laura M. Pérez, and Beatriz G. Gálvez

 

4. Ex Vivo Visualization and Analysis of the Muscle Stem Cell Niche

            Aviva J. Goel and Robert S. Krauss

 

5. Stem Cell-Derived Cardiac Spheroids as 3D In Vitro Models of the Human Heart Microenvironment

            Madeline Campbell, Mamta Chabria, Gemma A. Figtree, Liudmila Polonchuk, and Carmine Gentile

 

6. Isolation, Propagation, and Clonogenicity of Intestinal Stem Cells

            Prashanthi Ramesh, Aleksandar Buryanov Kirov, David Johannes Huels, and Jan Paul Medema

 

7. Isolation of Extracellular Vesicles from Subventricular Zone Neural Stem Cells

            Mary C. Morton, Victoria N. Neckles, and David M. Feliciano

 

8. Reconstruction of Regenerative Stem Cell Niche by Cell Aggregate Engineering

            Bing-Dong Sui, Bin Zhu, Cheng-Hu Hu, Pan Zhao, and Yan Jin

 

9. Three-Dimensional Co-Culture of Human Hematopoietic Stem/Progenitor Cells and Mesenchymal Stem/Stromal Cells in a Biomimetic Hematopoietic Niche Microenvironment

            Marta H.G. Costa, Tiago S. Monteiro, Susana Cardoso, Joaquim M.S. Cabral, Frederico Castelo Ferreira, and Cláudia L. da Silva

 

10. Investigating the Vascular Niche: Three-Dimensional Co-Culture of Human Skeletal Muscle Stem Cells and Endothelial Cells

            Claire Latroche, Michèle Weiss-Gayet, and Bénédicte Chazaud

 

11. Identification and Characterization of Stem Cells in Oral Cancer

            Sujit K. Bhutia, Prajna P. Naik, Prakash P. Praharaj, Debasna P. Panigrahi, Chandra S. Bhol, Kewal K. Mahapatra, Sarbari Saha, and Srimanta Patra

 

12. Determining Competitive Potential of Bone Metastatic Cancer Cells in the Murine Hematopoietic Stem Cell Niche

            Sun H. Park, Matthew R. Eber, Russell S. Taichman, and Yusuke Shiozawa

 

13. Spatial Genomic Analysis: A Multiplexed Transcriptional Profiling Method that Reveals Subpopulations of Cells within Intact Tissues

            Antti Lignell and Laura Kerosuo

 

14. 3D Culture of Mesenchymal Stem Cells in Alginate Hydrogels

            Sílvia J. Bidarra and Cristina C. Barrias

 

15. Isolation and Identification of Murine Bone Marrow-Derived Macrophages and Osteomacs from Neonatal and Adult Mice

            Joydeep Ghosh, Safa F. Mohamad, and Edward F. Srour

Blurb

This updated collection addresses the intense efforts that have been underway to characterize the cellular-molecular-biochemical elements of not only the hematopoietic stem cell niche but the niches for other stem cells. These specialized microenvironments that regulate stem cell function have continued to inspire tremendous interest as a subject of study in the years since the publication of the first edition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Stem Cell Niche: Methods and Protocols, Second Edition serves as an ideal guide for both experts and novices in the stem cell field.