1. In Vitro Maintenance of Multipotent Neural Crest Stem Cells as Crestospheres
Sofie Mohlin and Laura Kerosuo
2. Analysis of Hematopoietic Niche in the Mouse Embryo
Keai Sinn Tan, Nathalie Brouard, and Daisuke Sugiyama
3. Functional Assays of Stem Cell Properties Derived from Different Niches
Beatriz de Lucas, Laura M. Pérez, and Beatriz G. Gálvez
4. Ex Vivo Visualization and Analysis of the Muscle Stem Cell Niche
Aviva J. Goel and Robert S. Krauss
5. Stem Cell-Derived Cardiac Spheroids as 3D In Vitro Models of the Human Heart Microenvironment
Madeline Campbell, Mamta Chabria, Gemma A. Figtree, Liudmila Polonchuk, and Carmine Gentile
6. Isolation, Propagation, and Clonogenicity of Intestinal Stem Cells
Prashanthi Ramesh, Aleksandar Buryanov Kirov, David Johannes Huels, and Jan Paul Medema
7. Isolation of Extracellular Vesicles from Subventricular Zone Neural Stem Cells
Mary C. Morton, Victoria N. Neckles, and David M. Feliciano
8. Reconstruction of Regenerative Stem Cell Niche by Cell Aggregate Engineering
Bing-Dong Sui, Bin Zhu, Cheng-Hu Hu, Pan Zhao, and Yan Jin
9. Three-Dimensional Co-Culture of Human Hematopoietic Stem/Progenitor Cells and Mesenchymal Stem/Stromal Cells in a Biomimetic Hematopoietic Niche Microenvironment
Marta H.G. Costa, Tiago S. Monteiro, Susana Cardoso, Joaquim M.S. Cabral, Frederico Castelo Ferreira, and Cláudia L. da Silva
10. Investigating the Vascular Niche: Three-Dimensional Co-Culture of Human Skeletal Muscle Stem Cells and Endothelial Cells
Claire Latroche, Michèle Weiss-Gayet, and Bénédicte Chazaud
11. Identification and Characterization of Stem Cells in Oral Cancer
Sujit K. Bhutia, Prajna P. Naik, Prakash P. Praharaj, Debasna P. Panigrahi, Chandra S. Bhol, Kewal K. Mahapatra, Sarbari Saha, and Srimanta Patra
12. Determining Competitive Potential of Bone Metastatic Cancer Cells in the Murine Hematopoietic Stem Cell Niche
Sun H. Park, Matthew R. Eber, Russell S. Taichman, and Yusuke Shiozawa
13. Spatial Genomic Analysis: A Multiplexed Transcriptional Profiling Method that Reveals Subpopulations of Cells within Intact Tissues
Antti Lignell and Laura Kerosuo
14. 3D Culture of Mesenchymal Stem Cells in Alginate Hydrogels
Sílvia J. Bidarra and Cristina C. Barrias
15. Isolation and Identification of Murine Bone Marrow-Derived Macrophages and Osteomacs from Neonatal and Adult Mice
Joydeep Ghosh, Safa F. Mohamad, and Edward F. Srour
This updated collection addresses the intense efforts that have been underway to characterize the cellular-molecular-biochemical elements of not only the hematopoietic stem cell niche but the niches for other stem cells. These specialized microenvironments that regulate stem cell function have continued to inspire tremendous interest as a subject of study in the years since the publication of the first edition. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Stem Cell Niche: Methods and Protocols, Second Edition serves as an ideal guide for both experts and novices in the stem cell field.