Stem Cell Nanotechnology: Methods and Protocols

ISBN-13: 9781493963270 / Angielski / Miękka / 2017 / 184 str.

Kursad Turksen

Opis

This book features cutting-edge methods and protocols for nanotechnological approaches to stem cell biology. It provides step-by-step detail essential for reproducible results and contains key notes and implementation advice from experts in the field.

Spis treści

1. Propagation and Differentiation of Human Wharton’s Jelly Stem Cells on Three Dimensional Nanofibrous Scaffolds

            Kalamegam Gauthaman, Chui-Yee Fong, Jayarama Reddy Venugopal, Arijit Biswas, Seeram Ramakrishna, and Ariff Bongso

 

2. Composite Electrospun Nanofibers for Influencing Stem Cell Fate

            Alessandro Polini, Silvia Scaglione, Rodolfo Quarto, and Dario Pisignano

 

3. In Vitro Nanoparticle-Mediated Intracellular Delivery into Human Adipose-Derived Stem Cells

            Rosario Sanchez-Martin, Victoria Cano-Cortés, Juan Antonio Marchal, and Macarena Perán

 

4. Screening of Nanoparticle Embryotoxicity Using Embryonic Stem Cells

            Luisa Campagnolo, Ivana Fenoglio, Micol Massimiani, Andrea Magrini, and Antonio Pietroiusti

 

5. Peptide Nanofiber Scaffolds for Multipotent Stromal Cell Culturing

            Seher Ustun, Samet Kocabey, Mustafa O. Guler, and Ayse B. Tekinay

 

6. Patterned Polymeric Surfaces to Study the Influence of Nanotopography on the Growth and Differentiation of Mesenchymal Stem Cells

            Cristian Pablo Pennisi, Vladimir Zachar, Trine Fink, Leonid Gurevich, and Peter Fojan

 

7. Transduction of Murine Embryonic Stem Cells by Magnetic Nanoparticle-Assisted Lentiviral Gene Transfer

            Sarah Rieck,  Katrin Zimmermann, and Daniela Wenzel

 

8. Lanthanide-Based Upconversion Nanoparticles for Connexin-Targeted Imaging in Co-Cultures

            Sounderya Nagarajan and Yong Zhang

 

9. Basic Protocols to Investigate hMSC Behavior onto Electrospun Fibers

            Marco A. Alvarez-Perez,  Vincenzo Guarino, Valentina Cirillo, and Luigi Ambrosio

 

10. General Protocol for the Culture of Cells on Plasma-Coated Electrospun Scaffolds

            A. Géraldine Guex, Giuseppino Fortunato, Dirk Hegemann, Hendrik T. Tevaearai, and Marie-Noëlle Giraud

 

11. Mesenchymal Stem Cells and Nano-Structured Surfaces

            Yinghong Zhou, Nishant Chakravorty, Yin Xiao, and Wenyi Gu

 

12. Chondrogenic Differentiation of Menstrual Blood-Derived Stem Cells on Nanofibrous Scaffolds

            Somaieh Kazemnejad, Amir Hasan Zarnani, Manijeh Khanmohammadi, and Sahba Mobini

 

13. Engineering of a 3D Nanostructured Scaffold Made of Functionalized Self-Assembling Peptides and Encapsulated Neural Stem Cells

            Carla Cunha, Silvia Panseri, and Fabrizio Gelain

Blurb

Stem Cell Nanotechnology: Methods and Protocols gathers several representative protocols related to the emerging interest in nanotechnology as it relates to stem cell biology. The detailed chapters presented within have been validated for reproducibility and are described in an easy to follow, step-by-step fashion so as to be valuable for not only experts but also novices in the stem cell field.  As with other volumes in the highly successful Methods in Molecular Biology series, chapters conclude with a Notes section, which provides tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Stem Cell Nanotechnology: Methods and Protocols provides both a flavor of the field as it currently is and a source to stimulate new approaches and methodologies by those interested in nanotechnological applications.