Stem Cell Nanotechnology: Methods and Protocols

ISBN-13: 9781071603628 / Angielski / Miękka / 2021 / 214 str.

Kursad Turksen

Spis treści

1. Design of a Versatile Sample Holder for Facile Culture of Cells on Electrospun Membranes or Thin Polymer Films under Flow Conditions

            Anne-Sophie Mertgen, Markus Rottmar, Lukas Weidenbacher, and Anne Géraldine Guex

 

2. Single Cell Densitometry and Weightlessness Culture of Mesenchymal Stem Cells Using Magnetic Levitation

            Muge Anil-Inevi, Esra Yilmaz, Oyku Sarigil, H. Cumhur Tekin, and Engin Ozcivici

 

3. Fluorescent Nanoclusters for Imaging of Cells/Stem Cells

            Fatemeh Molaabasi, Behnam Hajipour-Verdom, Mozhgan Alipour, and Seyed Morteza Naghib

 

4. Protocol for Determining the Induction of Human Embryonic Stem Cells into Myogenic Lineage Using Electrospun Nanofibers

            Hasham S. Sofi, Nisar Ahmad Khan, and Faheem A. Sheikh

 

5. Preparation and Characterization of Simvastatin Nanocapsules: Encapsulation of Hydrophobic Drugs in Calcium Alginate

            Mazaher Ahmadi, Tayyebeh Madrakian, and Saeid Ghavami

 

6. Multifunctional Magnetic Nanoparticles-Labeled Mesenchymal Stem Cells for Hyperthermia and Bioimaging Applications

            Sadegh Ghorbanzade, Seyed Morteza Naghib, Ali Sadr, Fatemeh Sadat Fateminia, Ali Ghaffarinejad, Keivan Majidzadeh-A, and Amir Sanati-Nezhad

 

7. Synthesis of Hybrid Gold Nanoparticle (AuNP) Functionalized Superparamagnetic Nanoparticles (SPMNP) for Efficient Coupling of Biomolecules

            Deepak B. Thimiri Govinda Raj, Niamat Ali Khan, Srisaran Venkatachalam, Sivakumar Arumugam, and Selvamani Palaniswamy

 

8. Experimental Protocol for Induction of Transgene Expression in Neural Stem Cells through Polymeric Nanoparticles

            Taha Umair Wani, Hasham S. Sofi, Nisar Ahmad Khan, and Faheem A. Sheikh

 

9. Methodology Involved in the Osteogenic Differentiation of Mesenchymal Stem Cells on Chitosan-Collagen Nanofibers Incorporated with Titanium Dioxide Nanoparticles

            Roqia Ashraf, Hasham S. Sofi, and Faheem A. Sheikh

 

10. Experimental Protocol for Culture and Differentiation of Osteoblasts on 3D Abode Using Nanofiber Scaffolds

            Hasham S. Sofi, Roqia Ashraf, and Faheem A. Sheikh

 

11. Experimental Protocol of MSCs Differentiation into Neural Lineage for Nerve Tissue Regeneration Using Polymeric Scaffolds

            Roqia Ashraf, Hasham S. Sofi, and Faheem A. Sheikh

 

12. Fabrication and Electrospinning of 3D-Biodegradable Poly-L-Lactic Acid (PLLA) Nanofibers for Clinical Application

            Balasundari Ramesh, Kotturathu Mammen Cherian, and Adegbenro Omotuyi John Fakoya

 

13. Differentiation of Menstrual Blood Stem Cells into Keratinocyte-Like Cells on Bilayer Nano-Fibrous Scaffold

            Shaghayegh Arasteh, Sara Katebifar, Reza Shirazi, and Somaieh Kazemnejad

 

14. Dental Pulp Stem Cells in Customized 3D Nanofibrous Scaffolds for Regeneration of Peripheral Nervous System

            Siddhartha Das and Jayesh R. Bellare

 

15. Step-by-Step Protocol for Superparamagnetic Nanoparticle-Based Endosome and Lysosome Isolation from Eukaryotic Cell

            Deepak B. Thimiri Govinda Raj, Niamat Ali Khan, Srisaran Venkatachalam, Dinh Toi Chu, and Sivakumar Arumugam

 

16. Step-by-Step Protocol for Superparamagnetic Nanoparticle-Based Plasma Membrane Isolation from Eukaryotic Cell

            Deepak B. Thimiri Govinda Raj, Niamat Ali Khan, Srisaran Venkatachalam, and Dinh Toi Chu

 

17. Nanoscaled Materials for Drug Delivery into Cells/Stem Cells

            Sadegh Ghorbanzade and Seyed Morteza Naghib

 

18. Method for Evaluating Neuromodulatory Properties of Dental Pulp Stem Cell as an In Vitro Model for Parkinson’s Disease

            Nareshwaran Gnanasegaran, Deepak B. Thimiri Govinda Raj, and Sivakumar Arumugam

 

19. Detection and Monitoring of Stem Cell Differentiation Using Nanotechnology

            Esfandyar Askari and Seyed Morteza Naghib

 

20. BacMam System for Rapid Recombinant Protein Expression in Mammalian Cells

            Deepak B. Thimiri Govinda Raj

Blurb

This fully updated edition brings together a new set of protocols to arm stem cell biologists with tools and approaches to continue the quest to uncover the intricacies and regulatory circuits underlying stem cell biology. The collection explores the tremendous progress in our understanding of the potential of stem cells and the regulatory mechanisms controlling their fate, particularly through the parallel advances in nanotechnologies and their applications in biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Stem Cell Nanotechnology: Methods and Protocols, Second Edition serves a valuable tool for experts and novices alike in the vital field of stem cell research.