Stem Cell Migration: Methods and Protocols

ISBN-13: 9781617791444 / Angielski / Twarda / 2011 / 340 str.

Marie-Dominique Filippi; Hartmut Geiger

Opis

The migration of stem cells has been found to be critical during early development for the organization of the embryonic body as well as during adult life with tissue homeostasis and regeneration of organ function. Due to the low frequency of these cells in vivo, problems in identifying and prospectively purifying tissue specific stem cells near homogeneity, and, most importantly, a lack of adequate technologies and protocols to study stem cell migration in vivo, this vital research has been quite difficult until recently. In Stem Cell Migration: Methods and Protocols, experts in the field compile and highlight the standard and novel techniques that allow the studying of the migration of stem cells in one succinct manual. Including protocols on germ, neuronal, and hematopoietic stem cells, during development and adulthood with a clear emphasis on in vivo technologies, the volume also extends its coverage to in vitro approaches toward several developmentally-conserved signaling pathways. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and convenient, Stem Cell Migration: Methods and Protocols provides key, state-of the art information on experimental techniques for studying stem cell migration both at a cellular and molecular level in development, regeneration, and disease.

Recenzje

From the reviews:

"This well-illustrated book details the techniques essential for the understanding of the migration of hematopoietic stem cells, neural stem cells, and primordial germ cells. ... The book is well illustrated with both color and black-and-white figures. This is a highly informative manual of novel techniques dealing with stem cell migration both at a cellular and molecular level in development, regeneration, and disease. Students, researchers and physician-scientists interested in the areas of stem cell migration will find this book useful." (Omer Iqbal, Doody's Book Reviews, October, 2011)

Spis treści

Part I: Stem Cells and Migration: An Overview

 

1. Trafficking of Stem Cells

            Claire Magnon, Daniel Lucas, and Paul S. Frenette

 

2. Migratory Strategies of Normal and Malignant Stem Cells

            David A. Hess and Alison L. Allan

 

Part II: Stem Cells Identification and Microscopic Technologies to Track Transplanted Stem Cells In Vivo

 

3. Hematopoietic Stem Cell Characterization and Isolation

            Lara Rossi, Grant A. Challen, Olga Sirin, Karen Kuan-Yin Lin, and Margaret A. Goodell

 

4. Isolation and Characterization of Adult Neural Stem Cells

            Florian A. Siebzehnrubl, Vinata Vedam-Mai, Hassan Azari, Brent A. Reynolds, and Loic P. Deleyrolle

 

5. Magnetic Resonance Imaging of Stem Cell Migration

            Eva Syková Pavla Jendelová, and Vít Herynek

 

6. Imaging of Schwann Cells In Vivo

            Rahul Kasukurthi and Terrence M. Myckatyn

 

7. Imaging of Embryonic Stem Cell Migration In Vivo

            Andrew S. Lee and Joseph C. Wu

 

Part III: Stem Cell Migration During Development

 

8. In Vivo Germ Line Stem Cell Migration:  A Mouse Model

            Brian Dudley and Kathleen Molyneaux

 

9. Live Microscopy of Neural Stem Cell Migration in Brain Slices

            Jin-Wu Tsai and Richard B. Vallee

 

10. Whole Embryo Imaging of Hematopoietic Cell Emergence and Migration

            Michael J. Ferkowicz and Mervin C. Yoder

 

11. Stem Cell Migration: A Zebrafish Model

            Pulin Li and Leonard I. Zon

 

12. Imaging Pluripotent Cell Migration in Drosophila

            Michael J. Murray and Robert Saint

 

Part IV: Adult Hematopoietic Stem Cell Migration

 

13. Adhesion, Migration, and Homing of Murine Hematopoietic Stem Cells and Progenitors

            Jose A. Cancelas

 

14. Methods to Analyze the Homing Efficiency and Spatial Distribution of Hematopoietic Stem and Progenitor Cells and Their Relationship to the Bone Marrow Endosteum and Vascular Endothelium

            Jochen Grassinger and Susan K. Nilsson

 

15. Imaging Hematopoietic Stem Cells in the Marrow of Long Bones In Vivo

            Anja Köhler, Hartmut Geiger, and Matthias Gunzer

 

Part V: Non Hematopoietic Stem Cell Migration

 

16. Studies of Adult Neural Stem Cell Migration

            Adam C. Puche and Serena Bovetti

 

17. Dissecting Mesenchymal Stem Cell Movement: Migration Assays for Tracing and Deducing Cell Migration

            Erika L. Spaeth and Frank Marini

 

18. Epithelial Stem Cells

            Kyle M. Draheim and Stephen Lyle

 

Part VI: Pathways Implicated in Stem Cell Migration

 

19. Pathways Implicated in Stem Cell Migration:  The SDF-1/CXCR4 Axis

            Yaron Vagima, Kfir Lapid, Orit Kollet, Polina Goichberg, Ronen Alon, and Tsvee Lapidot

 

20. The Role of Receptor Tyrosine Kinases in Primordial Germ Cell Migration

            Louise Silver-Morse and Willis X. Li

 

21. Rho GTPases in Hematopoietic Stem/Progenitor Cell Migration

            Wei Liu, Yuxin Feng, Xun Shang, and Yi Zheng

Blurb

The migration of stem cells has been found to be critical during early development for the organization of the embryonic body as well as during adult life with tissue homeostasis and regeneration of organ function.  Due to the low frequency of these cells in vivo,  problems in identifying and prospectively purifying tissue specific stem cells near homogeneity, and, most importantly, a lack of adequate technologies and protocols to study stem cell migration in vivo, this vital research has been quite difficult until recently.  In Stem Cell Migration: Methods and Protocols, experts in the field compile and highlight the standard and novel techniques that allow the studying of the migration of stem cells in one succinct manual. Including protocols on germ, neuronal, and hematopoietic stem cells, during development and adulthood with a clear emphasis on in vivo technologies, the volume also extends its coverage to in vitro approaches toward several developmentally-conserved signaling pathways.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Practical and convenient, Stem Cell Migration: Methods and Protocols provides key, state-of the art information on experimental techniques for studying stem cell migration both at a cellular and molecular level in development, regeneration, and disease.