1. Introduction: Stem Cell Definition, Types of Stem Cells, Use and Application of Stem Cell 2. Extraction of Stem Cells from Animal Models 3. Harvest and Culture of Stem Cells 4. Establishment of Amniotic fluid Stem Cell 5. Stem Cell Characterization 6. Good Recording and Quality Control 7. Differentiation Techniques for Confirmation Studies 8. Transcriptomic Analysis 9. Proliferation Study and Analysis 10. Single Cell Isolation Methods and their Impact on Stem Cell Study 11. Gene Editing Techniques for Stem Cell 12. Preparation of Cells for in vitro study 13. Stem Cell Lab Design and Equipment 14. Human and Animal Research Ethics 15. Organoids