Stem Cell Heterogeneity: Methods and Protocols

ISBN-13: 9781493982349 / Angielski / Miękka / 2018 / 390 str.

Kursad Turksen

Spis treści

1. The Heterogeneity of Stem Cells: A Brief Overview

            Györgyi Műzes and Ferenc Sipos

 

2. Establishment and Characterization of Naïve Pluripotency in Human Embryonic Stem Cells

            Sharat Warrier, Mina Popovic, Margot Van der Jeught, and Björn Heindryckx

 

3. An Effective and Reliable Xeno-Free Cryopreservation Protocol for Single Human Pluripotent Stem Cells

            Guoliang Meng, Anna Poon, Shiying Liu, and Derrick E. Rancourt

 

4. Clonal Analysis of Cells with Cellular Barcoding: When Numbers and Sizes Matter

            Leonid V. Bystrykh and Mirjam E. Belderbos

 

5. Analysis of Cell Cycle Status of Murine Hematopoietic Stem Cells

            Krzysztof Szade, Karolina Bukowska-Strakova, Monika Zukowska, Alicja Jozkowicz, and Józef Dulak

 

6. Dissecting Transcriptional Heterogeneity in Pluripotency: Single Cell Analysis of Mouse Embryonic Stem Cells

            Ana M.V. Guedes, Domingos Henrique, and Elsa Abranches

 

7. Generation of Regionally Specific Neural Progenitor Cells (NPCs) and Neurons from Human Pluripotent Stem Cells (hPSCs)

            Josh Cutts, Nicholas Brookhouser, and David. A Brafman

 

8. Isolation and Culture of Embryonic Stem Cells, Mesenchymal Stem Cells, and Dendritic Cells from Humans and Mice

            Srabani Kar, Shinjini Mitra, and Ena Ray Banerjee

 

9. Decoding the Epigenetic Heterogeneity of Human Pluripotent Stem Cells with Seamless Gene Editing

            Amar M. Singh, Dustin W. Perry, Valeriya V. Adjan Steffey, Kenneth Miller, and Daniel W. Allison

 

10. Stencil Micropatterning for Spatial Control of Human Pluripotent Stem Cell Fate Heterogeneity

            Jun Yuan, Geetika Sahni, and Yi-Chin Toh

 

11. Visualizing the Functional Heterogeneity of Muscle Stem Cells

            Yasuo Kitajima, Shizuka Ogawa, and Yusuke Ono

 

12. Isolation and Expansion of Muscle Precursor Cells from Human Skeletal Muscle Biopsies

            Chiara Franzin, Martina Piccoli, Luca Urbani, Carlo Biz, Piergiorgio Gamba, Paolo De Coppi, and Michela Pozzobon

 

13. Measuring ATP Concentration in a Small Number of Murine Hematopoietic Stem Cells

            Krzysztof Szade, Monika Zukowska, Alicja Jozkowicz, and Józef Dulak

 

14. Growth Factor-Free Pre-Vascularization of Cell Sheets for Tissue Engineering

            Marina Costa, Rogério P. Pirraco, Mariana T. Cerqueira, Rui L. Reis, and Alexandra P. Marques

 

15. In Vitro Culture of Human Hematopoietic Stem Cells in Serum Free Medium and Their Monitoring by Flow Cytometry

            Marc Cloutier, Christine Jobin, Carl Simard, and Sonia Néron

 

16. Aerosol-Based Cell Therapy for Treatment of Lung Diseases

            Badrul Hisham Yahaya, Egi Kardia, and Nur Shuhaidatul Sarmiza Abdul Halim

 

17. Induction of Inner Ear Hair Cells from Mouse Embryonic Stem Cells In Vitro

            Masahide Yoshikawa and Yukiteru Ouji

 

18. Maintenance of Dermal Papilla Cells by Wnt-10b In Vitro

            Yukiteru Ouji and Masahide Yoshikawa

 

19. Maintenance of Skin Epithelial Stem Cells by Wnt-3a In Vitro

            Yukiteru Ouji and Masahide Yoshikawa

 

20. Enzyme-Free Dissociation of Neurospheres by a Microfluidic Chip-Based Method

            Ching-Hui Lin, Hao-Chen Chang, and Chia-Hsien Hsu

 

21. Automated Cell-Based Quantitation of 8-OHdG Damage

            Bilge Debelec-Butuner, Aykut Bostancı, Lisa Heiserich, Caroline Eberle, Filiz Ozcan, Mutay Aslan, Dirk Roggenbuck, and Kemal Sami Korkmaz

 

22. CoCl₂ Administration to Vascular MSC Cultures as an In Vitro Hypoxic System to Study Stem Cell Survival and Angiogenesis

            Carmen Ciavarella, Silvia Fittipaldi, and Gianandrea Pasquinelli

 

23. Reporter Systems to Study Cancer Stem Cells

            Caner Saygin, Mohamed Samour, Anastasia Chumakova, Awad Jarrar, Justin D. Lathia, and Ofer Reizes

 

24. Agent-Based Modeling of Cancer Stem Cell Driven Solid Tumor Growth

            Jan Poleszczuk, Paul Macklin, and Heiko Enderling

 

25. Induction of a Tumor-Metastasis-Receptive Microenvironment as an Unwanted Side Effect After Radio/Chemotherapy and In Vitro and In Vivo Assays to Study This Phenomenon

            Gabriela Schneider, Zachariah Payne Sellers, and Mariusz Z. Ratajczak

 

26. Isolation and Propagation of Glioma Stem Cells from Acutely Resected Tumors

            Jin-Kyu Jung, Mark R. Gilbert, and Deric M. Park

 

27. Isolation and Characterization of Cancer Stem Cells of the Non-Small Cell Lung Cancer (A549) Cell Line

            Nazilah Abdul Satar, Norashikin Zakaria, Noor Hanis Abu Halim, and Badrul Hisham Yahaya

Blurb

Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity. This goes against the long-held tenet that stem cells, defined by their capacity for self-renewal and lineage development, comprised a homogenous population, thus providing the reader with a new avenue of exploration into the complex world of stem cell study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Stem Cell Heterogeneity: Methods and Protocols serves as an ideal guide for investigators exploring this important area of research.