Stem Cell-Derived Models in Toxicology

ISBN-13: 9781493982646 / Angielski / Miękka / 2018 / 341 str.

Mike Clements; Liz Roquemore

Spis treści

1. Stem Cell‐Derived Models for Safety and Toxicity Assessments: Present and Future Studies in the “Proclinical Space”

            Gary Gintant and Stefan Braam

 

2. Human Pluripotent Stem Cell Test for Assessing the Potential Teratogen Risk

            Sei Kameoka and Eric Chiao

 

3. Cardiac Action Potential Measurement in Human Embryonic Stem Cell Cardiomyocytes for Cardiac Safety Studies Using Manual Patch-Clamp Electrophysiology

            Muthukrishnan Renganathan, Haiyang Wei, and Yong Zhao

 

4. Automated Patch Clamp Recordings of Human Stem Cell-Derived Cardiomyocytes

            Alison Obergrussberger, Claudia Haarmann, Sonja Stölzle-Feix, Nadine Becker, Atsushi Ohtsuki, Andrea Brüggemann, Michael George, and Niels Fertig

 

5. The CiPA Microelectrode Array Assay with hSC-Derived Cardiomyocytes: Current Protocol, Future Potential

            Daniel C. Millard, Mike Clements, and James D. Ross

 

6. Optogenetic Approach to Cardiotoxicity Screening: Simultaneous Voltage and Calcium Imaging Under Paced Conditions

            Graham T. Dempsey and Christopher A. Werley

 

7. HTS Compatible Voltage- and Ca²⁺-Sensitive Dye Recordings from hiPSC-Derived Cardiomyocytes Using the Hamamatsu FDSS Systems

            Ralf Kettenhofen

 

8. Kinetic Image Cytometry for Predicting Arrhythmias Using Human Stem Cell-Derived Cardiomyocytes

            Emily R. Pfeiffer, Ross Whittaker, Raquel Vega, Fabio Cerignoli, Patrick M. McDonough, and Jeffrey H. Price

 

9. Decoding Ca²⁺ Signals as a Non-Electrophysiological Method for Assessing Drug Toxicity in Stem-Cell Derived Cardiomyocytes

            Christopher H. George and David H. Edwards

 

10. Combined Impedance and Extracellular Field Potential Recordings from Human Stem Cell-Derived Cardiomyocytes

            Alison Obergrussberger, Ulrich Thomas, Sonja Stölzle-Feix, Nadine Becker, Krisztina Juhasz; Leo Doerr, Matthias Beckler, Michael George, and Niels Fertig

 

11. Edge Detection for Contractility Measurements with Cardiac Spheroids

            Christian Zuppinger

 

12. Contractile Force Readout of hESC-Cardiomyocytes

            Carlota Oleaga, Gregg Legters, L. Richard Bridges, Lee Kumanchik, Candace Martin, Yunqing Cai, Mark Schnepper, Christopher W. McAleer, Christopher J. Long, and James J. Hickman

 

13. In Vitro Cardiotoxicity Investigation Using High Content Analysis and Human Stem Cell-Derived Models

            Liz Roquemore, Ariel Kauss, Catherine Hather, Nick Thomas, and Hirdesh Uppal

 

14. Structural Toxicity: Hypertrophy Models of Human Pluripotent Stem Cell-Derived Cardiomyocytes

            Janos Kriston-Vizi, Sian E. Harding, and Gábor Földes

 

15. Addressing Functional Neurotoxicity Using the Microelectrode Array (MEA)

            Udo Kraushaar, Elke Guenther, and Dietmar Hess

 

16. Micropatterned Co-Cultures of Induced Pluripotent Stem Cell-Derived Hepatocytes and Stromal Cells for Drug Toxicity Studies

            Brenton R. Ware and Salman R. Khetani

Blurb

This detailed volume brings together a diverse collection of stem cell-derived model-based toxicity assays, from those routinely used to those deemed to have considerable potential. With a focus on differentiated tissues, the chapters explore numerous cardiotoxicity applications as well as coverage of neurotoxicity, hepatotoxicity, and more. Written for the Methods in Pharmacology and Toxicology series, the contents of this book aim to enable adoption of these protocols in laboratories that are interested in entering the field as well as to facilitate the transfer of best practices between laboratories that are already actively pursuing these technologies. 

Authoritative and cutting-edge, Stem Cell-Derived Models in Toxicology serves as a vital resource for researchers aiming to improve risk assessment in drug discovery and design.