Stem Cell-Based Neural Model Systems for Brain Disorders

ISBN-13: 9781071632864 / Angielski

Yu-Wen Alvin Huang; Changhui Pak

Spis treści

1. Generation of Cerebral Cortical Neurons from Human Pluripotent Stem Cells in 3D Culture

            Yuanwei Yan and Su-Chun Zhang

 

2. Generation of Homogeneous Populations of Cortical Interneurons from Human Pluripotent Stem Cells

            Peiyan Ni, Lingyi Fan, Amy Zinski, and Sangmi Chung

 

3. Generation and Co-Culture of Cortical Glutamatergic and GABAergic Induced Neuronal Cells

            Jay English, Danny McSweeney, Fumiko Ribbe, Ethan Howell, and ChangHui Pak

 

4. Transcription Factor-Directed Dopaminergic Neuron Differentiation from Human Pluripotent Stem Cells

            Yi Han Ng and Justyna A. Janas

 

5. Directed Differentiation of Human iPSCs into Microglia-Like Cells Using Defined Transcription Factors

            Shih-Wei Chen and Yu-Hui Wong

 

6. The Generation and Functional Characterization of Human Microglia-Like Cells Derived from iPS and Embryonic Stem Cells

            Mikael Lehoux, Kevin Connolly, Benedetta Assetta, and Yu-Wen Alvin Huang

 

7. Modeling Cellular Crosstalk of Neuroinflammation Axis by Tri-Cultures of iPSC-Derived Human Microglia, Astrocytes, and Neurons

            Kevin Connolly, Mikael Lehoux, Benedetta Assetta, and Yu-Wen Alvin Huang

 

8. Generation of Oligodendrocytes from Human Pluripotent and Embryonic Stem Cells

            Zoe Mattingly and Sundari Chetty

 

9. Characterizing the Neuron-Glial Interactions by the Co-Cultures of Human iPSC-Derived Oligodendroglia and Neurons

            Gabriella Vulakh and Xin Yang

 

10. Defined Differentiation of Human Pluripotent Stem Cells to Brain Microvascular Endothelial-Like Cells for Modeling the Blood-Brain Barrier

            Koji L. Foreman, Eric V. Shusta, and Sean P. Palecek

 

11. Modeling the Blood-Brain Barrier Using Human Induced Pluripotent Stem Cells

            Louise A. Mesentier-Louro, Natalie Suhy, Diede Broekaart, Michael Bula, Ana C. Pereira, and Joel W. Blanchard

 

12. A Three-Dimensional Primary Cortical Culture System Compatible with Transgenic Disease Models, Virally-Mediated Fluorescence, and Live Microscopy

            Sophie Brown, Elaina Atherton, and David A. Borton

 

13. Method to Generate Dorsal Forebrain Brain Organoids from Human Pluripotent Stem Cells

            Rebecca Sebastian, Narciso S. Pavon, Yoonjae Song, Karmen T. Diep, and ChangHui Pak

 

14. A 3D Bioengineered Neural Tissue Model Generated from Human iPSC-Derived Neural Precursor Cells

            Selene Lomoio and Giuseppina Tesco

 

15. FACS-Based Sequencing Approach to Evaluate Cell Type to Genotype Associations Using Cerebral Organoids

            Liam Murray, Meagan N. Olson, Nathaniel Barton, Pepper Dawes, Yingleong Chan, and Elaine T. Lim

 

16. Dynamic Measurement of Endosome-Lysosome Fusion in Neurons Using High-Content Imaging

            Qing Ouyang, Michael Schmidt, and Eric M. Morrow

 

17. Live-Imaging Detection of Multivesicular Body-Plasma Membrane Fusion and Exosome Release in Cultured Primary Neurons

            Matthew F. Pescosolido, Qing Ouyang, Judy S. Liu, and Eric M. Morrow

 

18. Assays of Monitoring and Measuring Autophagic Flux for iPSC-Derived Human Neurons and Other Brain Cell Types

            Ryan O’Rourke, Guzide Ayse Erdemir, and Yu-Wen Alvin Huang

 

19. Measuring Neuronal Network Activity Using Human Induced Neuronal Cells

            Danny McSweeney, Jay English, Ethan Howell, Fumiko Ribbe, and ChangHui Pak

 

20. A Simple Ca²⁺-Imaging Approach of Network-Activity Analyses for Human Neurons

            Zijun Sun

 

21. Whole Cell Patch Clamp Electrophysiology in Human Neuronal Cells

            Rafael Gabriel III, Andrew J. Boreland, and Zhiping P. Pang

 

22. Assaying Chemical Long-Term Potentiation in Human iPSC-Derived Neuronal Networks

            Deborah Pré, Alexander T. Wooten, Haowen Zhou, Ashley Neil, and Anne G. Bang

Blurb

This detailed volume presents validated and well-adapted procedures involving humanized and/or stem cell-based neural model systems that have proven helpful in better understanding the essential brain functions involved in the pathogenesis of brain disorders. The book explores the generation of multiple neural cell types in 2D and 3D as well as cutting-edge techniques to assay neural function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Stem Cell-Based Neural Model Systems for Brain Disorders serves as an essential resource for researchers and students in neuroscience, stem cell biology, and related fields.