Spermatogonial Stem Cells: Methods and Protocols

ISBN-13: 9781071631386 / Angielski / Twarda / 2023 / 420 str.

Jon Oatley; Brian P. Hermann

Opis

This volume explores the latest techniques used in the field of mammalian germ cell biology to study key experimental approaches and perspectives for critical analysis of spermatogonial subsets including the rare spermatogonial stem cells (SSCs) pool.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding common pitfalls.

Cutting-edge and comprehensive, Spermatogonial Stem Cells: Methods and Protocols is a valuable resource for both new and experiences investigators looking to expand their knowledge in this developing field.  

Spis treści

Preface…
Table of Contents…
Contributing Authors…

1. Introduction: The Why’s and How’s for Studying Spermatogenesis and Spermatogonial Stem Cells
Brian P. Hermann and Jon M. Oatley

2. Optimizing Testicular Fixation-Embedding Procedures for an Improved Morphofunctional Evaluation of Mammalian Spermatogonia
Helio Chiarini-Garcia and Andre L. Caldeira-Brant

3. Single-Molecule Fluorescence In Situ Hybridization for Spatial Detection of mRNAs in Sections of Mammalian Testes
Victoria D. Diaz and Brian P. Hermann

4. Bulk and Single-Cell RNA-Seq Analyses for Studies of Spermatogonia
Anukriti Singh and Brian P. Hermann

5. Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell – Part 1: Epigenomics Assays
Keren Cheng and John McCarrey

6. Profiling the Epigenetic Landscape of the Spermatogonial Stem Cell – Part 2: Computational Analysis of Epigenomics Data
Keren Cheng and John R. McCarrey

7. Primary Cultures of Spermatogonia and Testis Cells
Fan Yang, Jiachen Sun, and Xin Wu

8. Reconstitution of Human Prospermatogonial Development from Human Induced Pluripotent Stem Cells
Yasunari Seita, Youngsun Hwang, and Kotaro Sasaki

9. RNA Interference as a Method of Gene Knockdown in Cultured Spermatogonia
Connor Cason and Tessa Lord

10. Isolation of Undifferentiated Spermatogonia from Adult and Developing Mouse Testes
Vera van Melis, Lorena Roa de la Cruz, and Brian P. Hermann

11. Spermatogonial Transplantation
Makoto Nagano and Xiangfan Zhang

12. Viral Transduction of Mammalian Spermatogonial Stem Cells
Makiko Yamada, Yanyun Luo, and Marco Seandel

13. Pharmacological Treatment of Neonatal and Juvenile Mice to Study Spermatogenesis
Oleksandr Kirsanov and Christopher B. Geyer

14. Models and Methods for Evaluating Regeneration of Spermatogenesis After Cytotoxic Treatments 
Gunapala Shetty

15. Defining Gene Function in Spermatogonial Stem Cells through Conditional Knockout Approaches 
Julien M. D. Legrand and Robin M. Hobbs

16. Lineage Tracing of Spermatogonial Stem Cells within the Male Germline
Nathan Law

17. Perspectives: Approaches for Studying Livestock Spermatogonia
Michela Ciccarelli and Jon M. Oatley

18. Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells
Sarah K. Munyoki and Kyle E. Orwig

Subject Index List…