Spectrophotometry in Pharmaceutical Analysis

ISBN-13: 9783844392715 / Angielski / Miękka / 100 str.

Shabbir Ahmed; Akhtar Rasul; Zeeshan Masood

Opis

A simple spectrophotometric method for determination of cefaclor in raw and dosage forms based on reaction with ninhydrin is developed, optimized and validated. The purple color (Ruhemenn s purple) resulted from the reaction was stabilized and measured at 560 nm.The method optimization was essential as many procedural parameters influenced the accuracy of determination all variables including the reagent concentration, heating time, reaction temperature, color stability period, and cefaclor/ninhydrin ratio were studied in order to optimize the reaction conditions. No interference was observed from common pharmaceutical adjuvant. The developed method is easy to use, accurate and highly cost-effective for routine studies relative to HPLC and other techniques.

Drug analysis plays an important role in the development of drugs,their manufacture and therapeutic use. Pharmaceutical industries rely upon quantitative chemical analysis to ensure that the raw material used and final products obtained meet the required specifications.