Spectral and Imaging Cytometry: Methods and Protocols

ISBN-13: 9781071630198 / Angielski / Twarda / 2023 / 238 str.

Natasha S. Barteneva; Ivan A. Vorobjev

Opis

This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of Imagestream and also introduces FlowCam – cytometer with capabilities of analyzing phytoplankton and zooplankton. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations.

Cutting-edge and thorough, Spectral and Imaging Flow Cytometry: Methods and Protocols, Second Edition is a valuable resources for beginners and professionals interested in learning more about this developing field. 

Chapters 1, 2, and 5 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Spis treści

Dedication…
Preface…
Table of Contents…
Contributing Authors…

Part I Spectral Flow Cytometry

1. Development of Spectral Imaging Cytometry
Ivan A. Vorobjev, Aigul Kussanova, and Natasha S. Barteneva

2. Using Virtual Filtering Approach to Discriminate Microalgae by Spectral Flow Cytometer
Natasha S. Barteneva, Aigul Kussanova, Veronika Dashkova, Ayagoz Meirkhanova, and Ivan A. Vorobjev

Part II Imaging Flow Cytometry - Imagestream

3. Imaging Flow Cytometric Analysis of Primary Bone Marrow Erythroblastic Islands
Joshua Tay, Kavita Bisht, Ingrid G. Winkler, Jean-Pierre Levesque

4. Imaging Flow Cytometry of Legionella-Containing Vacuoles in Intact and Homogenized Wild-Type and Mutant Dictyostelium
Amanda Welin, Dario Hüsler, and Hubert Hilbi

5. Imaging Flow Cytometry of Multi-Nuclearity
Ivan A. Vorobjev, Sultan Bekbayev, Adil Temirgaliyev, Madina Tlegenova, and Natasha S. Barteneva

6. The Imaging Flow Cytometry-Based Cytokinesis-Block MicroNucleus (CBMN) Assay 
Ruth C. Wilkins, Matthew Rodrigues, and Lindsay A. Beaton-Green

7. High-Throughput γ-H2AX Assay Using Imaging Flow Cytometry
Younghyun Lee, Qi Wang, Ki Moon Seong, and Helen C. Turner

8. Label Free Identification of Persistent Particles in Association with Primary Immune Cells by Imaging Flow Cytometry
Bradley Vis, Jonathan J. Powell, and Rachel E. Hewitt

9. “Immuno-FlowFISH": Applications for Chronic Lymphocytic Leukaemia
Henry Y.L. Hui, Wendy N. Erber, and Kathy A. Fuller

10. Quantifying Golgi Apparatus Fragmentation Using Imaging Flow Cytometry 
Inbal Wortzel and Ziv Porat

11. Flow Imaging of the Inflammasome: Evaluating ASC Speck Characteristics and Caspase-1 Activity 
Abhinit Nagar and Jonathan A. Harton

12. Quantitative Analysis of Latex Beads Phagocytosis by Human Macrophages Using Imaging Flow Cytometry with Extended Depth of Field (EDF) 
Ekaterina Pavlova, Daria Shaposhnikova, Svetlana Petrichuk, Tatiana Radygina, and Maria Erokhina

Part III Imaging Flow Cytometry - FlowCam

13. FlowCam 8400 and FlowCam Cyano Phytoplankton Classification and Viability Staining by Imaging Flow Cytometry
Kathryn H. Roache-Johnson and Nicole R. Stephens

14. Optimizing FlowCam Imaging Flow Cytometry Operation for Classification and Quantification of Microcystis Morphospecies
Dmitry Malashenkov, Veronika Dashkova, Ivan A. Vorobjev, and Natasha S. Barteneva

Subject Index List…

Blurb

This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of Imagestream and also introduces FlowCam – cytometer with capabilities of analyzing phytoplankton and zooplankton. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations.

Cutting-edge and thorough, Spectral and Imaging Cytometry: Methods and Protocols, Second Edition is a valuable resources for beginners and professionals interested in learning more about this developing field. 

Chapters 1, 2, and 5 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.