Sorghum: Methods and Protocols

ISBN-13: 9781493990382 / Angielski / Twarda / 2019 / 281 str.

Zuo-Yu Zhao; Jeff Dahlberg

Spis treści

Preface…

Table of Contents…

Contributing Authors…

Part I Breeding Technologies

1. Hybrid Sorghum Product Development and Production

Larry Lambright

2. Mapping QTLs and Identification of Genes Associated with Drought Resistance in Sorghum

Karen R. Harris-Shultz, Chad M. Hayes, and Joseph E. Knoll

3. Field Studies to Develop Weed Management Programs for Grain Sorghum

J. Anita Dille

4. Discovery of Sorghum Haploid Induction System

Tanveer Hussain and Cleve Franks

5. Development of a Pedigreed Sorghum Mutant Library

Junping Chen, Guihua Zou, and Zhanguo Xin

6. Marker Assisted Selection in Sorghum using KASP Assay for Detection of Single Nucleotide Polymorphism/Insertion Deletion

Gloria Burow, Ratan Chopra, Halee Hughes, Zhanguo Xin, and John Burke

Part II Grain Compounds, Nutrition, and Digestibility

7. Assaying Sorghum Nutritional Quality

Kwaku G. Duodu

8. Tannin Analysis in Sorghum Grains

Linda Dykes

9. Sorghum Phytochemicals and their Potential Impact on Human Health

Linda Dykes

10. Identification and Quantification of Carotenoids and Tocochromanols in Sorghum Grain by High Performance Liquid Chromatography

Darwin Ortiz and Mario G. Ferruzzi

Part III Biotechnologies

11. Gene Editing in Sorghum through Agrobacterium

Jeffry D. Sander

12. Genome Editing by CRISPR/Cas9 in Sorghum through Biolistic Bombardment

Guoquan Liu, Jieqing Li, and Ian D. Godwin

13. Novel Ternary Vectors for Efficient Sorghum Transformation

Ajith Anand, Ping Che, Emily Wu, and Todd J. Jones

14. Nutritionally Enhanced Sorghum for the Arid and Semi-Arid Tropical Areas of Africa

Zuo-yu Zhao, Ping Che, Kimberly Glassman, and Marc Albertsen

15. Evaluation of Agronomic Performance of β-Carotene Elevated Sorghum in Confined Field Conditions

Ping Che, Zuo-Yu Zhao, Mark Hinds, Kristen Rinehart, Kimberly Glassman, and Marc Albertsen

Part IV Economics and Broad Application

16. Economics of Increasing Sorghum Productivity in Sub-Saharan Africa: The Mali Case

John H. Sanders, Botorou Ouendeba, Ababacar Ndoye, Niaba Témé, and Soungalo Traore

17. Background on Grain Sorghum Usage

John Duff, Austin Vincent, Doug Bice, and Ian Hoeffner

18. Assaying Sorghum for Fuel Production

Courtney Payne, Justin Sluiter, and Ed Wolfrum

19. The Role of Sorghum in Renewables and Biofuels

Jeff Dahlberg

Blurb

This book details sorghum breeding technologies, grain compounds, nutrition and digestibility, biotechnology methods, broad renewable applications and an economic study.  Chapters are divided into five review chapters, five case study chapters, and nine protocol chapters providing comprehensive reviews, new study results or state-of-the-art protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Sorghum: Methods and Protocols aims to provide useful information and tools to an array of readers looking to research and utilize sorghum.