Somatic Hybridization in Crop Improvement II

ISBN-13: 9783642625008 / Angielski / Miękka / 2012 / 378 str.

Toshiyuki Nagata;Y.P.S. Bajaj

Opis

This richly illustrated volume describes how somatic hybrids can contribute to the improvement of crops. It comprises 24 chapters dealing with interspecific and intergeneric somatic hybridization and cybridization, providing valuable tools for plant breeders.

Spis treści

Section I Cereals.- I.1 Somatic Hybridization Between Hordeum vulgare L. (Barley) and Daucus carota L. (Carrot).- 1 Introduction.- 2 Protoplast Fusion and Culture of Fused Cells.- 3 Analysis of the Three Regenerated Plants.- 4 Characterization of Somatic Hybrids.- 5 Quantification and Effects of Betaine.- 6 Discussion.- 7 Protocol.- References.- I.2 Cybridization in ryza sativa L. (Rice).- 1 Introduction.- 2 Somatic Hybridization.- 3 Charcteriztion of Rice Cybrids.- 4 Selection of CMS Cybrid Plants.- 5 Application of the Cybridization Method in Rice Breeding.- 6 Summary and Conclusion.- 7 Protocol.- References.- I.3 Somatic Hybridization Between Oryza sativa L. (Rice) and Hordeum vulgare L. (Barley).- 1 Introduction.- 2 Protoplast Fusion and Culture.- 3 Analysis of Regenerated Plant.- 4 Assessment of Cold andSalt Tolerance.- 5 Protocol.- References.- I.4 Somatic Hybridization Between Triticum aestivum L. (Wheat) and Haynaldia villosa L..- 1 Introduction.- 2 Preparation and Treatment of Parental Protoplasts.- 3 Protoplast Fusion and Culture.- 4 Verification of Hybridity.- 5 Growth and Development of Hybrid Plants.- 6 Summary and Conclusion.- 7 Protocol.- References.- I.5 Asymmetric Somatic Hybridization Between Triticum aestivum L. (Wheat) and Leymus chinensis (Trin.) Tzvel.- 1 Introduction.- 2 Preparation and Inactivation of Wheat Protoplasts.- 3 Preparation of L. chinensis Protoplasts from Irradiated Calli.- 4 Fusion of Parental Protoplasts and Culture of Fusion Products.- 5 Identification of Hybrid Calh and Plants.- 6 Summary and Conclusion.- 7 Protocol.- References.- Section II Vegetables and Fruits.- II.1 Somatic Hybridization Between Arabidopsis and Brassica.- 1 Introduction.- 2 Somatic Hybridization.- 3 Summary and Conclusion.- 4 Protocol.- References.- II.2 Somatic Hybridization in Asparagus.- 1 Introduction.- 2 Somatic Hybridization.- 3 Summary and Conclusion.- 4 Protocol.- References.- II.3 Somatic Hybridization in Cichorium intybus L. (Chicory).- 1 Introduction.- 2 Somatic Hybridization.- 3 Analysis of the Regenerated Plants.- 4 Summary and Conclusion.- 5 Protocol for the Regenaration of Somatic Hybrids.- References.- II.4 Cybridization in Citrus unshiu Marc. (Satsuma Mandarin) and C sinensis (L.) Osbeck (Sweet Orange).- 1 Introduction.- 2 Somatic Hybridization.- 3 Summary and Conclusion.- 4 Protocol.- References.- II.5 Somatic Hybridization in Cucumis.- 1 Introduction.- 2 Somatic Hybridization.- 3 Summary and Conclusion.- 4 Protocol.- References.- II.6 Somatic Hybridization in Diospyros (Persimmon).- 1 Introduction.- 2 Somatic Hybridization.- 3 Summary and Conclusion.- 4 Protocol.- References.- II.7 Somatic Hybridization in Ipomoea (Sweet Potato) Species.- 1 Introduction.- 2 Protoplast Isolation.- 3 Protoplast Culture and Plant Regeneration.- 4 Interspecific Hybridization Between Sweet Potato and Wild Relatives.- 5 Asymmetric Protoplast Fusion Between Sweet Potato (Ipomoea batatas Lam.) and African Marigold (Tagetes erecta L.).- 6 Summary and Conclusion.- 7 Protocol for Asymmetric Protoplast Fusion Between Sweet Potato (Ipomoea batatas Lam.) and Wild Relative, J. trifida Don.- References.- II.8 Somatic Hybridization Between Lycopersicon esculentum Mill. (Tomato) and Wild Nontuberous Solanum Species.- 1 Introduction.- 2 Methods of Protoplast Isolation, Protoplast Culture, and Plant Regeneration.- 3 Production and Analysis of Intergeneric Somatic Hybrids.- 4 Summary and Conclusion.- 5 Protocol.- References.- II.9 Somatic Hybridization Between Lycopersicon esculentum Mill. (Tomato) and Solanum melongena L. (Eggplant).- 1 Introduction.- 2 Somatic Hybridization.- 3 Analysis of Somatic Hybrids.- 4 Elimination of the Donor Genome.- 5 Polyploidization and Elimination of the Donor Genome.- 6 Protocol.- References.- II.10 Somatic Hybridization Between Lycopersicon esculentum Mill. (Tomato) and Solanum ochranthum Dun..- 1 Introduction.- 2 Isolation of Protoplasts.- 3 Culture of Protoplasts.- 4 Fusion of Protoplasts and Regeneration of Somatic Hybrids.- 5 Regeneration and Characterization of Somatic Hybrids.- 6 Summary and Conclusion.- 7 Protocol.- References.- II.11 Somatic Hybridization Between Solanum melongena L. (Eggplant) and Solanum sanitwongsei Craib..- 1 Introduction.- 2 Somatic Hybridization.- 3 Summary and Conclusions.- 4 Protocol.- References.- II.12 Somatic Hybridization Between Solanum commersonii Dun. and S. tuberosum L. (Potato).- 1 Introduction.- 2 Somatic Hybridization.- 3 Summary and Conclusion.- 4 Protocol.- References.- II.13 Somatic Hybridization Between Solanum tuberosum L. (Potato) and Solanum phureja.- 1 Introduction.- 2 Somatic Hybridization.- 3 Summary and Conclusion.- References.- Section III Medicinal and Aromatic Plants.- III.1 Somatic Hybridization in Dianthus Species.- 1 Introduction.- 2 Protoplast Isolation.- 3 Protoplast Culture.- 4 Protoplast Fusion.- 5 Regeneration of Somatic Hybrids.- 6 Summary and Conclusion.- 7 Protocol.- References.- III.2 Somatic Hybridization Between Nicotiana sylvestris Speg. & Comes and N. plumbaginifolia Viv,.- 1 Introduction.- 2 Somatic Hybridization.- 3 Applications of Somatic Hybrids.- 4 Summary and Conclusion.- 5 Protocol.- References.- III.3 Somatic Hybridization Between Nicotiana tabacum L. (Tobacco) and Atropa belladonna L. (Deadly Nightshade).- 1 Introduction.- 2 Somatic Hybridization.- 3 Conclusions and Prospects.- 4 Protocol.- References.- III.4 Somatic Hybridization and Cell Grafting in Senecio.- 1 Introduction.- 2 Results and Discussion.- 3 Summary and Conclusion.- 4 Protocol.- References.- Section IV Legumes/Pasture Crops.- IV.1 Somatic Hybridization Between Medicago sativa L. (Alfalfa) and Lotus corniculatus L. (Birdsfoot Trefoil).- 1 Introduction.- 2 Symmetric Somatic Cell Hybridization.- 3 Asymmetrie Somatic Cell Hybridization.- 4 Summary and Conclusion.- 5 Protocol.- References.- IV.2 Somatic Hybridization Between Medicago sativa L. (Alfalfa) and Medicago falcata L..- 1 Introduction.- 2 Isolation and Fusion of Protoplasts.- 3 Culture and Selection of Fused Protoplasts.- 4 Regeneration of Somatic Hybrids.- 5 Indentification and Characterization of Somatic Hybrid Plants.- 6 Summary and Conclusion.- 7 Protocol.- References.