Somatic Cell Nuclear Transfer Technology

ISBN-13: 9781071630631 / Angielski

Marcelo Tigre Moura

Opis

This volume details protocols on animal cloning by Somatic cell nuclear transfer for basic research and biotechnological applications. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Somatic Cell Nuclear Transfer Technology aims to be comprehensive guide for researchers.

Spis treści

1.    Cloning by SCNT: integrating technical and biology-driven advances

Marcelo T. Moura

 

2.    Epigenetic reprogramming and somatic cell nuclear transfer

            Luna N. Vargas, Márcia M. Silveira and Maurício M. Franco

 

3.    Early cell specification in mammalian fertilized and somatic cell nuclear transfer embryos

            Marcelo D. Goitis and Jose B. Cibelli

 

4.    Mitochondrial inheritance following nuclear transfer: from cloned animals to patients with mitochondrial disease

            Jörg P. Burgstaller and Marcos R. Chiaratti

 

5.    Stem cells as nuclear donors for mammalian cloning

            Carolina G. Silva and Carlos F. Martins

 

6.    Animal Transgenesis and cloning: combined development and future perspectives

            Melissa S. Yamashita and Eduardo O. Melo

           

7.    Mouse cloning using outbred oocyte donors and non-toxic reagents

            Sayaka Wakayama, Yukari Terashita, Yoshiaki Tanabe, Naoki Hirose and Teruhiko Wakayama

 

8.    Somatic cell nuclear transfer in rabbits

            Pengxiang Qu, Wenbin Cao and Enqi Liu

 

9.    Production of cloned pigs by handmade cloning

            Gábor Vajta, Wen B. Chen and Zoltan Machaty

 

10.  Somatic Cell Nuclear Transfer in Pigs

            Werner G. Glanzner, Vitor B. Rissi and Vilceu Bordignon

 

11.  Somatic cell nuclear transfer using freeze-dried protaminized donor nuclei

            Luca Palazzese, Marta Czernik, Kazutsugu Matsukawa and Pasqualino Loi

 

12.  Cattle cloning by somatic cell nuclear transfer

            Juliano R. Sangalli, Rafael V. Sampaio, Tiago H. C. De Bem, Lawrence C. Smith and Flávio V. Meirelles

 

13.  Production of water buffalo SCNT embryos by handmade cloning

            Prabhat Palta, Naresh L. Selokar and Manmohan S. Chauhan

 

14.  Bovid interspecies somatic cell nuclear transfer with ooplasm transfer

            Antonio L. González-Grajéales and Gabriela F. Mastromonaco

 

15.  Horse somatic cell nuclear transfer using zona pellucida intact and zona-free oocytes

            Daniel Salamone and Marc Maserati

 

16.  A modified handmade cloning method for dromedary camels

            Fariba Moulavi and Sayyed M. Hosseini

 

17.  Derivation of bovine primed embryonic stem cells from somatic cell nuclear transfer embryos

            Delia A. Soto, Micaela Navarro and Pablo J. Ross