Skin Tissue Engineering: Methods and Protocols

ISBN-13: 9781493994724 / Angielski / Twarda / 2019 / 263 str.

Sophie Bottcher-Haberzeth; Thomas Biedermann

Spis treści

PART I Isolation and culture of skin cells

 

1 Isolation and Culture of Human Keratinocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Sergio Cortez Ghio, Gaëtan Le-Bel, Amélie Lavoie, Danielle Larouche, and Lucie Germain

 

2 Promotion of Human Epidermal Keratinocyte Expansion in Feeder Cell Co-culture. . . . . . . . . . . . . . . . . .

Daisuke Suzuki, Filipa Pinto, and Makoto Senoo

 

3 Isolation and Culture of Epidermal Melanocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Muriel Cario, and Alain Taieb

 

4 Long-term Expansion of Mouse Primary Epidermal Keratinocytes Using a TGF-beta Signaling Inhibitor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Filipa Pinto, Daisuke Suzuki, and Makoto Senoo

 

5 Isolation and Culture of Hair Follicle Dermal Sheath Mesenchymal Stromal Cells . . . . . . . . . . . . . . . . . . . .

Dongrui Ma, Seng-Teik Lee, and Alvin Wen Choong Chua

 

6 Isolation and Culture of Human Dermal Fibroblasts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Agnes S. Klar

 

7 Isolation and Culture of Human Dermal Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . Jennifer Bourland, Dominique Mayrand, Nathalie Tremblay, Véronique J. Moulin, Julie Fradette, and François A. Auger

 

8 Isolation of Stromal Vascular Fraction by Fractionation of Adipose Tissue . . . . . . . . . . . . . . . . . . . . . . . . . . Joris A. van Dongen, Martin C. Harmsen, and Hieronymus P. Stevens

 

PART II Tissue engineering skin and engineered skin models

 

9  Engineering a Multi-Layered Skin Equivalent: the Importance of Endogenous Extracellular Matrix Maturation to Provide Robustness and Reproducibility. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Lydia Costello, Nicola Fullard, Mathilde Roger, Steven Bradbury, Teresa DiColandrea, Robert Isfort, Charles Bascom, and Stefan Przyborski

 

10 Three-dimensional Epidermal Model from Human Hair follicle-derived Keratinocytes . . . . . . . . . . . . . 

Takamitsu Matsuzawa, Michiyo Nakano, Ayako Oikawa, Yuumi Nakamura, and Hiroyuki Matsue

 

11 Fabrication of a Co-Culture System with Human Sweat Gland Derived Cells and Peripheral Nerve Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Matthias Brandenburger, and Charli Kruse

 

12 Engineering a Multilayered Skin Substitute with Keratinocytes, Fibroblasts, Adipose Derived Stem Cells and Adipocytes . . . . . . .  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Maike Keck, Alfred Gugerell, and Johanna Kober

 

13 Skin Tissue Engineering Fabrication of Chimeric Hair Follicles for Skin Tissue Engineering . . . . . . . .

Andrea L. Lalley, and Steven T. Boyce

 

14 Isolation and Culture of Epidermolysis Bullosa Cells and Organotypic Skin Models. . . . . . . . . . . . . . . . .

Yinghong He, Cristina Has

 

 

PART III In vitro and vivo models testing skin, skin cells, and tissue engineered skin

 

15 Effects of the Extracellular Matrix on the Proteome of Primary Skin Fibroblasts . . . . . . . . . . . . . . . . . . .

Regine C. Tölle, and Jörn Dengjel

 

16 Standard Preparation Protocol of Human Skin Samples for Transmission Electron Microscopy . . . .

Gery Barmettler, and Urs Ziegler

 

17 Methods for Assessing Scaffold Vascualarization In Vivo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Jiang-Hui Wang, Jinying Chen, Shyh-Ming Kuo, Geraldine M. Mitchell, Shiang Y. Lim, and Guei-Sheung Liu

 

18 Human Reconstructed Skin in a Mouse Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Jun Mi, Shuai Chen, Lin Xu, Jie Wen, Xin Xu, and Xunwei Wu

 

19 Pig Model to Test Tissue Engineered Skin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Christian Tapking, Daniel Popp, and Ludwik K. Branski

 

20 Transplantation of Autologous Dermo-Epidermal Skin Substitutes in a Pig Model . . . . . . . . . . . . . . . . .

Thea Fleischmann, Flora Nicholls, Miriam Lipiski, Margarete Arras, and Nikola Cesarovic

Blurb

This volume provides protocols describing the isolation and culture of diverse cell types stemming from the skin and the use of these cells and cell constructs for wound healing, bioengineering applications, and translational medicine purposes. The book is divided into three sections describing the isolation and culture of diverse skin cells, managing these cells within co-culture systems and skin models, as well as using these skin models in a test setting. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Skin Tissue Engineering: Methods and Protocols serves as a vital aid to basic and clinical researchers such as biologists, physicians, and biomedical engineers working with and being interested in basic science, and clinically and laboratory-applicable translational regenerative medicine.