Skin Stem Cells: Methods and Protocols

ISBN-13: 9781493988693 / Angielski / Twarda / 2019 / 463 str.

Kursad Turksen

Opis

This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal lineages, which have led to the isolation of numerous stem cell-like sub-populations from the epidermis and dermis.

Spis treści

1. In Vivo Genetic Alteration and Lineage Tracing of Single Stem Cells by Live Imaging

            Olivia Farrelly, Paola Kuri, and Panteleimon Rompolas

 

2. Ex Vivo Imaging and Genetic Manipulation of Mouse Hair Follicle Bulge Stem Cells

            Daniel Haensel, Melissa A. McNeil, and Xing Dai

 

3. Quantitative Detection of Low-Abundance Transcripts at Single-Cell Level in Human Epidermal Keratinocytes by Digital Droplet Reverse Transcription-Polymerase Chain Reaction

            Frédéric Auvré, Julien Coutier, Michèle T. Martin, and Nicolas O. Fortunel

 

4. Qualitatively Monitoring Binding and Expression of the Transcription Factors Sp1 and NFI as a Useful Tool to Evaluate the Quality of Primary Cultured Epithelial Stem Cells in Tissue Reconstruction

            Gaëtan Le-Bel, Sergio Cortez Ghio, Danielle Larouche, Lucie Germain, and Sylvain L. Guérin

 

5. Ribonucleoproteins Mediated Efficient In Vivo Gene Editing in Skin Stem Cells

            Wenbo Wu and Ting Chen

 

6. Isolation and Characterization of Cutaneous Epithelial Stem Cells

            Stephanie R. Gillespie and David M. Owens

 

7. Interfollicular Epidermal Stem Cells: Boosting and Rescuing from Adult Skin

            Mariana T. Cerqueira, Ana M. Frias, Rui L. Reis, and Alexandra P. Marques

 

8. Whole-Mount Immunofluorescent Staining Coupled to Multicolour Lineage Tracing Model for Analyzing the Spatio-Temporal Organization of Epidermal Stem Cells

            Edwige Roy and Kiarash Khosrotehrani

 

9. Isolation and Enrichment of Newborn and Adult Skin Stem Cells of the Interfollicular Epidermis

            Stefano Sol, Dario Antonini, and Caterina Missero

 

10. Isolation and Cultivation of Epidermal (Stem) Cells

            Xusheng Wang, Shiyang Dong, and Yaojiong Wu

 

11. One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen

            Hua Qian, Xue Leng, Jie Wen, Qian Zhou, Xin Xu, and Xunwei Wu

 

12. Isolation and Cultivation of Skin-Derived Precursors

            Xiaoxiao Wang, Shiyang Dong, and Yaojiong Wu

 

13. Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells

            Petek Korkusuz, Sevil Köse, Nilgün Yersal, and Selin Önen

 

14. Isolation of Human Skin Epidermal Stem Cells Based on the Expression of Endothelial Protein C Receptor

            Meilang Xue, Suat Dervish, and Christopher J. Jackson

 

15. Decellularized bSIS-ECM as a Regenerative Biomaterial for Skin Wound Repair

            Mahmut Parmaksiz, Ayşe Eser Elçin, and Yaşar Murat Elçin

 

16. Protocols for Full Thickness Skin Wound Repair Using Prevascularized Human Mesenchymal Stem Cell Sheet

            Lei Chen, Daniel Radke, Shaohai Qi, and Feng Zhao

 

17. Cultivation of Adipose-Derived Stromal Cells on Intact Amniotic Membrane-Based Scaffold for Skin Tissue Engineering

            Ehsan Taghiabadi, Bahareh Beiki, Nasser Aghdami, and Amir Bajouri

 

18. Amniotic Membrane Seeded Fetal Fibroblasts as Skin Substitute for Wound Regeneration

            Ehsan Taghiabadi, Bahareh Beiki, Nasser Aghdami, and Amir Bajouri

 

19. Skin Wound Healing: Refractory Wounds and Novel Solutions

            Gabriel M. Virador, Lola de Marcos, and Victoria M. Virador

 

20. Isolation, Culture, and Motility Measurements of Epidermal Melanocytes from GFP-Expressing Reporter Mice

            Lina Dagnino and Melissa Crawford

 

21. Melanoblasts as Multipotent Cells in Murine Skin

            Tsutomu Motohashi and Takahiro Kunisada

 

22. Regeneration of Mouse Skin Melanocyte Stem Cells In Vivo and In Vitro

            Ke Yang, Weiming Qiu, Pei-Rong Gu, and Mingxing Lei

 

23. Interactions between Epidermal Keratinocytes, Dendritic Epidermal T-Cells, and Hair Follicle Stem Cells

            Krithika Badarinath, Abhik Dutta, Akshay Hegde, Neha Pincha, Rupali Gund, and Colin Jamora

 

24. Isolating Immune Cells from Mouse Embryonic Skin

            Ambika S. Kurbet and Srikala Raghavan

 

25. Direct Conversion of Mouse Embryonic Fibroblasts into Neural Crest Cells

            Tsutomu Motohashi and Takahiro Kunisada

 

26. High-Titer Production of HIV-Based Lentiviral Vectors in Roller Bottles for Gene and Cell Therapy

            Hazal Banu Olgun, Hale Tasyurek, Ahter Dilsad Sanlioglu, and Salih Sanlioglu

 

27. High Grade Purification of Third-Generation HIV-Based Lentiviral Vectors by Anion Exchange Chromatography for Experimental Gene and Stem Cell Therapy Applications

            Hazal Banu Olgun, Hale Tasyurek, Ahter D. Sanlioglu, and Salih Sanlioglu

 

28. Full-Thickness Human Skin Equivalent Models of Atopic Dermatitis

            Gopu Sriram, Paul Lorenz Bigliardi, and Mei Bigliardi-Qi

 

29. Human Hair Follicle Associated-Pluripotent (hHAP) Stem Cells Differentiate to Cardiac Muscle Cells

            Robert M. Hoffman

 

30. Isolation of Normal Fibroblasts and Their Cancer-Associated Counterparts (CAFs) for Biomedical Research

            Barbora Dvořánková, Lukáš Lacina, and Karel Smetana Jr.

 

31. Isolation of Cancer Stem Cells from Squamous Cell Carcinoma

            Silvia Fontenete and Mirna Perez-Moreno

 

32. Identification of Human Cutaneous Squamous Cell Carcinoma Cancer Stem Cells

            Carlotta Olivero, Huw Morgan, and Girish K. Patel

 

33. Identification of Human Cutaneous Basal Cell Carcinoma Cancer Stem Cells

            Huw Morgan, Carlotta Olivero, and Girish K. Patel

Blurb

This detailed book collects new and updated techniques that will help to improve our understanding of the mechanisms underlying stem cell-derived repair through stem cells of the epidermal and dermal lineages, which have led to the isolation of numerous stem cell-like sub-populations from the epidermis and dermis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and practical, Skin Stem Cells: Methods and Protocols, Second Edition serves as a valuable assemblage of protocols for researchers both already working in the field and those who now wish to newly embark on studies of skin stem cells.