Skeletal Muscle Regeneration in the Mouse: Methods and Protocols

ISBN-13: 9781493938087 / Angielski / Twarda / 2016 / 346 str.

Michael Kyba

Opis

Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing theprogenitor cells of skeletal muscle, and quantifying overall muscle function.

Spis treści

Part I Injury Models

 

1.  Eccentric Contraction-Induced Muscle Injury: Reproducible, Quantitative, Physiological Models to Impair Skeletal Muscle’s Capacity to Generate Force
Jarrod A. Call and Dawn A. Lowe

 

2.  Volumetric Muscle Loss
Beth E. Pollot and Benjamin T. Corona

3. Freeze Injury of the Tibialis Anterior Muscle
Gengyun Le, Dawn A. Lowe, and Michael Kyba

4.  Synergist Ablation as a Rodent Model to Study Satellite Cell Dynamics in Adult Skeletal Muscle
Tyler J. Kirby, John J. McCarthy, Charlotte A. Peterson, and Christopher S. Fry

 

5.  Inducing and Evaluating Skeletal Muscle Injury by Notexin and Barium Chloride
Matthew T. Tierney and Alessandra Sacco

6.  Cardiotoxin Induced Injury and Skeletal Muscle Regeneration
Glynnis A. Garry, Marie Lue Antony, and Daniel J. Garry

7.  Fibrosis-Inducing Strategies in Regenerating Dystrophic and Normal Skeletal Muscle
Patrizia Pessina and Pura Muñoz-Cánoves  

Part II Evaluating Progenitor Cells

8.  Isolation, Cryosection, and Immunostaining of Skeletal Muscle
Huascar P. Ortuste Quiroga, Katsumasa Goto, and Peter S. Zammit

9.  Isolation of Mouse Periocular Tissue for Histological and Immunostaining Analyses of the Extraocular Muscles and their Satellite Cells
Pascal Stuelsatz and Zipora Yablonka-Reuveni

10. Skeletal Muscle Tissue Clearing for LacZ and Fluorescent Reporters, and Immunofluorescence Staining
Mayank Verma, Bhavani Sai Rohit Murkonda, Yoko Asakura, and Atsushi Asakura

11. Isolation, Culture, Functional Assays and Immunofluorescence of Myofiber-Associated Satellite Cells
Thomas O. Vogler, Katherine E. Gadek, Adam B. Cadwallader, Tiffany L. Elston, and Bradley B. Olwin

12. Transplantation-Based Quantitative Assays for Satellite Cell Self-Renewal and Differentiation
Robert W. Arpke and Michael Kyba

13. Non-Invasive Tracking of Quiescent and Activated MuSC Engraftment Dynamics In Vivo
Andrew T.V. Ho and Helen M. Blau

14. Myogenic Progenitors from Mouse Pluripotent Stem Cells for Muscle Regeneration
Alessandro Magli, Tania Incitti, and Rita C.R. Perlingeiro

15. Assaying Human Myogenic Progenitor Cell Activity by Reconstitution of Muscle Fibers and Satellite Cells in Immunodeficient Mice
Maura H. Parker

16. Methods for Mitochondria and Mitophagy Flux Analyses in Stem Cells of Resting and Regenerating Skeletal Muscle
Laura García-Prat, Marta Martínez-Vicente, and Pura Muñoz-Cánoves

17. Identification, Isolation, and Characterization of Mesenchymal Progenitors in Mouse and Human Skeletal Muscle
Akiyoshi Uezumi, Takehiro Kasai, and Kunihiro Tsuchida

18. FACS Fractionation and Differentiation of Skeletal-Muscle Resident Multipotent Tie2+ Progenitors
Arpita A. Biswas and David J. Goldhamer

Part III Muscle Functional Assays

19. In Vitro Assays to Determine Skeletal Muscle Physiologic Function
Justin E. Sperringer and Robert W. Grange

20. In Vivo Assessment of Muscle Contractility in Animal Studies
Shama R. Iyer, Ana P. Valencia, Erick O. Hernández-Ochoa, and Richard M. Lovering

21. Functional Measurement of Respiratory Muscle Motor Behaviors Using Transdiaphragmatic Pressure
Sarah M. Greising, Carlos B. Mantilla, and Gary C. Sieck

22. Assessment of the Contractile Properties of Permeabilized Skeletal Muscle Fibers
Dennis R. Claflin, Stuart M. Roche, Jonathan P. Gumucio, Christopher L. Mendias, and Susan V. Brooks

23. Analysis of Aerobic Respiration in Intact Skeletal Muscle Tissue by Microplate-Based Respirometry
Jonathan Shintaku and Denis C. Guttridge

O autorze

Michael Kyba, Ph.D. Associate Professor and Lillehei Endowed Scholar, Lillehei Heart Institute NHH4.126, University of Minnesota, SE Minneapolis, MN 55455, USA

Blurb

This volume focuses on the cell biology and physiology of skeletal muscle regeneration. This Book is a collection of classic and cutting edge protocols optimized for mice, but in most cases adaptable to rat or other mammalian models, that will allow an investigator to develop and implement a research study on skeletal muscle regeneration. Chapters address the three major areas of study: provoking regeneration by inducing damage to muscle, analyzing the progenitor cells of skeletal muscle, and quantifying overall muscle function. Subjects discussed include: inducing skeletal muscle injury by eccentric contraction; volumetric muscle loss; single myofiber isolation and culture; satellite cell transplantation; muscle clearing for whole mount immunostaining; luciferase tracking of muscle stem cells; mitochondrial and mitophagy flux analysis; in vivo assessment of muscle contractility; force measurements on single isolated myofibers; and analysis of aerobic respiration in intact skeletal muscle tissue by microplate respirometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to each respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and practical, Skeletal Muscle Regeneration in the Mouse: Methods and Protocols is an essential laboratory reference for research in skeletal muscle growth, damage, repair, degeneration, and regenerative therapy in the mouse model system.