. Generating Genetically Modified Mice: A Decision Guide
Ivo J. Huijbers
2. Direct Generation of Conditional Alleles using CRISPR/Cas9 in Mouse Zygotes
Colin Pritchard, Lona J. Kroese, and Ivo J. Huijbers
3. Building Cre Knockin Rat Lines using CRISPR/Cas9
Yuanwu Ma, Lianfeng Zhang, and Xingxu Huang
4. Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases
Eugenia Voziyanova, Rachelle P. Anderson, and Yuri Voziyanov
5. Use of the DICE Dual Integrase Cassette Exchange System
Alfonso P. Farruggio, Mital S. Bhakta, and Michele P. Calos
6. Ligand-controlled Site-specific Recombination in Zebrafish
Avinash Chekuru, Veronika Kuscha, Stefan Hans, and Michael Brand
7. Injection-based Delivery of Cell Permeable Peptide-tagged Cre
Wei-Ming Chien, Yonggang Liu, Ana A. Dinca, and Michael T. Ch
in
8. Viral Delivery of GFP-dependent Recombinases to the Mouse Brain
Jonathan C.Y. Tang, Stephanie Rudolph, and Constance L. Cepko
9. Recombinase-mediated Cassette Exchange using Adenoviral Vectors
Andreas F. Kolb, Christopher Knowles, Patrikas Pultinevicius, Jennifer A. Harbottle, Linda Petrie, Claire Robinson, and David A. Sorrell
10. Marker Removal in Transgenic Plants using Cre recombinase Delivered with
Potato Virus X
Lilya Kopertekh and Joachim Schiemann
11. Nanoparticle-mediated Recombinase Delivery into Maize
Susana Martin-Ortigosa, Brian G. Trewyn, and Kan Wang
12. Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines
Qi Luand Zhuo-Hua Pan
13. FLPing Genes On and Off in Drosophila
Bonnie M. Weasn
er, Jinjin Zhu, and Justin P. Kumar
14. Imaging Neural Architecture in Brainbow Samples
Douglas H. Roossien and Dawen Cai
15. Purification and invitro characterization of Zinc Finger Recombinases
Femi J. Olorunniji, Susan J. Rosser, and W. Marshall Stark
16. Preparing Mate-paired Illumina Libraries using Cre Recombinase
Ze Peng
17. Preparing Fosmid Mate-Paired Libraries Using Cre-LoxP Recombination
Ze Peng, Jeff L. Froula, and Jan-Fang Cheng
18. Using Purified Tyrosine Site-specific Recombinases In Vitro to Rapidly Construct and Diversify Metabolic Pathways
Wei Liu, Laura R. Tuck, Jon Marles Wright,and Yizhi Cai
19. Multipart DNA Assembly Using Site-specific Recombinases from the Large Serine Integrase Family
Femi J. Olorunniji, Christine Merrick, Susa
n J. Rosser, Margaret C. M. Smith, W. Marshall Stark, and Sean D. Colloms
20. Production of Minicircle DNA Vectors using site Specific Recombinases
Nafiseh Sanei Ata-abadi, Naeimeh Rezaie, Kianoush Dormiani, Mohammad Hossein, and Nasr-Esfahani
This books aims to provide an up-to-date guide to using recombinases in the laboratory. Chapters guide the reader through construction of transgenic animals, recombinase-mediated cassette exchange (RMCE), spatial control of recombinase expression and in vivo delivery approaches, immunohistochemistry and fluorescence-based phenotyping methods, biochemical methods, and recombinase-based production of minimal DNA vectors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Site-Specific Recombinases: Methods and Protocols aims to serve as a valuable resource for working with recombinases, and inspires further creativity in the field.